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Citations for Thermo Fisher :
1301 - 1350 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNA samples were prepared for gel electrophoresis by adding 1 μl loading dye (gel loading buffer II, Invitrogen) followed by incubation at 95°C for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: Approximately 10μg of each protein extract was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermofisher) and transferred to PVDF membrane (GE10600022 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA integrity was determined after visualised on an agarose gel stained with SYBR Green nucleic acid gel stain (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The digested PCR products were subjected to gel purification using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific). The gel-purified PCR products were assembled together with the ampicillin marker fragment using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 12% polyacrylamide-gel electrophoresis (PAGE) gels in 1x TBE running buffer and were visualized by staining with SYBR Gold II (Invitrogen) stain or fluorescence imager.
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of detergent-soluble lysates were resolved using the NuPAGE Novex Midi Gel system on 4–12% Bis–Tris gels (Invitrogen). Proteins were transferred to PVDF membranes (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were purified by preparative agarose gel electrophoresis and extracted with GeneJET Gel Extraction Kit purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved by gel electrophoresis using the NuPage Bis-Tris 4-12% precast gel system according to the manufacturer’s instructions (Life Technologies). Western blot transfer was carried out according to the manufacturer’s instructions with the SureLock X-cell system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on acrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were then incubated with 10,000-fold diluted SYBR Gold nucleic acid gel stain (Life technologies, Eugene, OR, Catalog# S11494) in 0.5× TBE buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... A subset of PCR products were visualized on a 1% agarose gel stained with SYBR Safe DNA gel stain (Invitrogen) using 5 μl product with 1 μl 5X loading dye (BioLine ...
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bioRxiv - Biochemistry 2020Quote: ... 18 μg of total protein were resolved under reducing conditions by using SDS polyacrylamide gels and NuPage Novex 4–12% Bis/Tris Gels (Invitrogen) for SUMO conjugates analysis or 10% for SAE2 immunodetection ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of DNA extracts were assessed for quantity and quality by gel electrophoresis using 0.8% (w/v) agarose gels and a Qubit® 2.0 Fluorometer (Invitrogen, USA), using high-sensitivity reagents.
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bioRxiv - Microbiology 2021Quote: ... using AccuStart™ II PCR ToughMix (QuantaBio) and subsequent gel electrophoresis on a 1.5% agarose gel with Invitrogen 100 bp DNA Ladder (ThermoFisher Scientific).
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bioRxiv - Biophysics 2021Quote: The purified proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% Bis-Tris NuPAGE gels (Invitrogen). Prior to SDS-PAGE analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... boiled for 5 min and size-fractionated by gel electrophoresis on NuPAGE 10% Bis-Tris 1.5-mm gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Pooled DNA was separated on a 1% TAE-agarose gel and desired band extracted using PureLink Quick Gel Extraction Kit (Invitrogen). The extracted DNA was cleaned and concentrated using a DNA Clean & Concentrator Kit (Zymo ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were supplemented with 4 μL of 6x loading buffer (30% glycerol, bromphenol blue, xylene cyanol) and were resolved by polyacrylamide gel electrophoresis in 4-12% TBE Gels (Invitrogen) in 1%TBE buffer at 100 V for 45 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Samples were loaded onto 8% SDS-polyacrylamide gels or 4–12% Tris/HEPES-SDS Bolt™ precast gels (Thermo-Invitrogen) for electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
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bioRxiv - Cell Biology 2019Quote: ... SDS PAGE and electrotransfer were performed on 4-12% Bis-Tris gel (Novex) using mini gel tank and iBlot transfer systems (Invitrogen). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... We analyzed 40 μg of protein by SDS-polyacrylamide gel electrophoresis on an 8% Tris-Glycine gel (Thermo Fisher Scientific) and transferred overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... was separated by polyacrylamide gel electrophoresis with lithium dodecyl sulfate (SDS-PAGE) on NuPAGE 4%–12% Bis-Tris Gel (Invitrogen). For the analysis of the GFP-tagged proteins ...
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bioRxiv - Biochemistry 2020Quote: ... (S) were resolved in 10% denaturing gels and the gels were stained with GelCode Blue Stain Reagent (Thermo Fisher Scientific) and imaged.
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bioRxiv - Physiology 2020Quote: Protein electrophoresis was performed with 10% SDS-PAGE gel in Tris-glycine-SDS running buffer or 4-12 % Bis-Tris gradient gels in MOPS-SDS running buffer (Invitrogen). Proteins were transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were loaded in SDS-PAGE gel NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo fisher) along with molecular weight marker ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were run on a 1.5% agarose gel and visualized with SYBR Safe DNA gel stain (Invitrogen, 1x final concentration). RNR bands were cut out ...
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bioRxiv - Microbiology 2020Quote: ... Samples were run on a 1.5% agarose gel and visualized with SYBR Safe DNA gel stain (Invitrogen, 1x final concentration). Fusion products were observed in the unfused mix control when no blocking primers were used ...
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bioRxiv - Synthetic Biology 2020Quote: ... All pieces were run on 1 or 2% (w/v) Agarose gel stained with SYBR Safe DNA gel stain (S33102, Invitrogen). Bands at expected sizes were isolated from the gel (740609.50 ...
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bioRxiv - Cell Biology 2019Quote: ... For resolving native septin complexes 4–16% Novex NativePAGE Bis-Tris Gel System precast poly¬acrylamide gels (Invitrogen, Carlsbad, CA) were used ...
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bioRxiv - Developmental Biology 2019Quote: ... 50-100 μg of total protein extracts were solubilized in SDS-sample buffer by boiling at 95°C for 5 minutes and analysed by SDS polyacrylamide gel electrophoresis (4-12% NuPAGE gel; Invitrogen). Western blotting was performed using antibodies against Vasa (rat ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were subjected to SDS-PAGE on NuPage 4-12% Bis-tris gels Bis-tris gels (Thermo Fisher Scientific # NP0336BOX) and transferred to nitrocellulose membrane (Bio-Rad #1704271) ...
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bioRxiv - Cell Biology 2020Quote: A total of 20 μg of protein was processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a 4-12% Bis-Tris Mini-gel (Invitrogen) and the 3-(N-morpholino ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplicons were visualized and imaged using a FluorChemQ imager (ProteinSimple) on a 2% agarose gel with SYBR Safe DNA gel stain (Invitrogen) and excised using a blue light transilluminator (Clare Chemical Research) ...
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bioRxiv - Biochemistry 2021Quote: ... 1-4 μg of total RNA was loaded on 1% agarose-glyoxal gel prepared with NorthernMax-Gly gel prep running buffer (Ambion) and transferred to nylon membranes (BrightStar-Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10% β-mercaptoethanol at 70°C for 10min and separated by gel electrophoresis (NuPAGE 4-12% Bis-Tris gel with 1X MES running buffer, ThermoFisher). Gels were transferred to nitrocellulose membranes (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 1cm x 1cm gel pieces were cut from the expanded gel and attached on 24 mm round Poly-D-Lysine (A3890401, Gibco) coated coverslips to prevent gel from sliding and to avoid drifting while imaging ...
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bioRxiv - Genetics 2021Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and by quantitative PCR (SYBR green, Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were loaded on either a 4-12% Bis-Tris gel or a 4-20% Tris-Glycine gel (Invitrogen), and SDS gel electrophoresis was performed as previously described (96 ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-resistant and irreversibly assembled protein conjugates were visualized using Novex NuPAGE 12% bis-tris SDS–PAGE (polyacrylamide gel electrophoresis) gels (Invitrogen) run at 4°C in a NuPAGE MES SDS running buffer (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting samples were separated by electrophoresis on 2.5% agarose gel and signals were detected using SYBR™ Gold Nucleic Gel Stain (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on polyacrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... the same amounts of SOSIP trimer and SOSIP-I53-50NP were loaded on a 4-12% Bis-Tris NuPAGE gel or 3-12% Bis-Tris NuPAGE gel (both from Invitrogen), respectively.
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bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were cleaned from gel using NucleoSpin Gel and PCR cleanup (MN) and cloned in TOPO-TA plasmids (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... Fourteen μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF membranes (Millipore ...