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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human KLRD1 protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 50ng/mL recombinant murine EGF (Thermo Fisher; PMG8041), 100ng/mL recombinant human Noggin (VWR International ...
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bioRxiv - Cell Biology 2023Quote: ... before RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) was added to all samples at a final concentration of 8 U·μl-1 with an incubation at room temperature for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662), guinea pig anti-bovine insulin (Linco ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Molecular Biology 2021Quote: ... and Western blotting was performed with anti-His (ThermoFisher MA1-135), anti-HA (Roche 11583816001) ...
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bioRxiv - Developmental Biology 2020Quote: ... The Ion Hi-Q Sequencing 200 Kit (Thermo Fisher Scientific, UK) and the Ion Proton platform were used for analysis ...
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bioRxiv - Cell Biology 2021Quote: ... pRSET-His-GFP-3C-Survivin (pRSET vector from Thermo Fisher Scientific), pMCNcs-INCENP1-58 and pETM-His-TEV-Borealin10-280 or pETM-His-TEV-Borealin10-221 (pETM vector ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was bound to His-Pur Cobalt resin (Thermo Scientific) for 2 hours with rocking at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The Ion PGMTM Hi-QTM View OT2 kit (Life Technologies, USA) and Ion OneTouch DL instrument (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
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bioRxiv - Zoology 2019Quote: ... Blots were probed with rabbit anti-His antibody (Thermo Fisher Scientific) at 1 ...
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bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... and His-BMAL1 FL were co-infected into monolayer HighFive (Invitrogen) insect cells for protein expression ...
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
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bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Cancer Biology 2021Quote: ... The HA-tagged cDNAs were subcloned into the pLenti6-D-TOPO vector (Invitrogen). The cDNA for human GSK3βwas subcloned into the pCMV- myc vector ...