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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Cow Peripherin PRPH ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were collected for ELISA assays and RNA was extracted from infected cells using Trizol (ThermoFisher; catalog no. 15596026) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with the DENV-2 NGC strain was coated onto Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed 3 times and then developed by adding 100 µl/well of TMB-ELISA substrate (ThermoFisher Scientific, catalog# 34029). Reactions were stopped after 10 minutes by adding 50 µl/well of 3M H2SO4 to all the wells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Venom was diluted in 100 mM carbonate-bicarbonate coating buffer pH 9.6 and coated at 100 ng per well in 96-well ELISA plates (Nunc MaxiSorp, ThermoScientific) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... content in isolated mitochondria from WT and APP cells was measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer instructions (ThermoFisher khb3544).
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bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was taken at 590 nm with a reference filter of 620 nm using an ELISA reader (Multiskan Spectrum Microplate Reader, Thermo Scientific). All data were normalized against DMSO blank controls.
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bioRxiv - Cancer Biology 2023Quote: ... Using the standard curve from the ELISA and the amount of gDNA/sample (quantified by Nanodrop Spectrophotometer from Thermo Fisher Scientific), percentage of methylated DNA (5-mC ...
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bioRxiv - Molecular Biology 2023Quote: ... except for antibodies used for detection in ELISA and flow cytometry. Genetic constructs were synthesized by Eurofins Genomics. Plasmid DNA midiprep (cat. K210004) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies; Grand Island ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Levels of Aβ40 in supernatants of HEK or HEKswe cells transfected with different MT5-MMP variants and/or C99 constructs or treated with C3 or DAPT were evaluated by ELISA (#KHB3481, Thermo Fisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
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bioRxiv - Bioengineering 2020Quote: ... The blood samples were then centrifuged at 2000 xg for 10 minutes and serum collected for TNF determination by ELISA (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Recombinant trimeric ectodomain of S protein with six proline mutations (rtS-ecto6P) for enzyme-linked immunosorbent assay (ELISA) was produced using Expi293F cells (Thermo Fisher Scientific). In addition to the mutation at the furin cleavage site ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol with some adjustments: Thermo Scientific Nunc MaxiSorp 96 well ELISA plates were coated with 50 μg/mL Calf thymus DNA (Thermo Fisher Scientific), Phosphorylcholine (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the plate was centrifuged to pellet down the bacterial cells and 75 µL of the supernatant from each well was transferred to a 96-well ELISA-plate (Thermo Fisher Scientific) containing 25 µL of binding buffer (0.0258 M sodium carbonate ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed 3 times with TBS-T and phage binding was detected by addition of 20 µl SuperSignal™ ELISA Pico Chemiluminescent Substrate (Thermo-Fisher Scientific) and read on an Envision multilabel plate reader (Perkin-Elmer) ...
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bioRxiv - Biochemistry 2023Quote: ... After centrifugation screening of soluble EDB-specific binders was performed by ELISA as follows: lysates were incubated with target-coated (67B89) and off-target-coated (6789) 96-well Medisorp-plates (Nunc, Roskilde, Denmark) followed by washing with PBST and PBS ...
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bioRxiv - Immunology 2023Quote: ELISA to detect specific anti-NP IgG1 antibodies was performed in 96-well flat bottom Nunc-ImmunoTM MaxiSorpTM plates (Thermo Fisher Scientific). Plates were incubated ON using 10 μg/mL of NP(2,5)-BSA or NP(25)-BSA as the coating reagent kindly provided by Dr ...
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bioRxiv - Bioengineering 2022Quote: ... Dam serum and placental tissue lysates (both prepared as described above) were assayed for IL-6 concentration using an ELISA per manufacturer instructions (Invitrogen, Waltham, MA). Sample absorbance values were compared to a standard curve to calculate IL-6 concentration.
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bioRxiv - Microbiology 2023Quote: ... The final step was the addition of 100 µL of 1-Step Ultra TMB-ELISA Substrate solution (Thermo Scientific, catalogue number 34029) into wells for 5 minutes to allow a colorimetric reaction ...
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bioRxiv - Cell Biology 2023Quote: ... ELISA signal was measured using a BioTek Synergy Neo microplate reader one minute after adding SuperSignal ELISA Pico Chemiluminescent substrate (ThermoFisher Scientific, 37069).
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bioRxiv - Immunology 2023Quote: ... Blood from all animals of an exposure group was pooled and centrifuged (600 x g) and serum isolated and assessed for immunoglobulin E (IgE) and IgG2a using sandwich ELISA (Invitrogen, Waltham, MA) according to manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... Bacteria from day two plates were resuspended in PBS and adjusted to an OD 600 nm of 0.5 to coat high binding ELISA plates (Nunc-Immuno™, ThermoScientific). After incubation overnight at 4ºC ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBS and developed by adding 100 µL of 1-Step Turbo TMB ELISA (Thermo Fisher Scientific) for 30 minutes at RT followed by stopping the reaction with 50 µL of 2M H2SO4 and reading optical density at 450 nm using BioTek ELx800 plate reader.
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The reaction was stopped with 3 N H2SO4 and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa).
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bioRxiv - Molecular Biology 2019Quote: The protein concentrations of the ELISA were corrected for the total protein concentrations of the sample as determined by Qubit fluorescence incorporation protocol (Qubit, Life Technologies, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Immunology 2021Quote: ... Cross-link activation of reporter cells was performed by direct coating of target antibody to ELISA plate (Nunc Maxisorp; 96 well, flat transparent), followed by a blocking step and incubation with 2 × 105 reporter cells per well ...
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bioRxiv - Immunology 2022Quote: ... IL-5 and IFN-γ were quantified using Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ (Thermo Fisher Scientific, Waltham, MA, USA). IP-10 (CXCL10) ...
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bioRxiv - Immunology 2020Quote: Culture supernatants at different time intervals post infection/ treatment were filtered through a 0.2μ filter and subjected to ELISA by using the eBioscience (Thermo Fisher Scientific Inc. USA) ELISA kit as per recommended protocols.
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bioRxiv - Cell Biology 2020Quote: The concentration of IFNλ1/IL-29 was measured in basolateral medium from RSV BT2a- or mock-infected cultures at 96 hpi by ELISA (Thermo Fisher Scientific; BMS2049). The manufacturer’s instructions were followed.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was developed using TMB (3,3⍰,5,5⍰-Tetramethylbenzidine) substrate (1-Step™ Ultra TMB-ELISA Substrate Solution; Thermo Scientific, Waltham, MA, USA). Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific ...