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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 6 METHOXY 1 2 3 4 TETRAHYDRO NAPHTHALENE 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg DNA and 6 µL PEI were incubated with 50 µL Opti-MEM (Gibco) seperately for 5 minutes before combining and incubating for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The culture medium was replaced with fura-2 AM (6 μM, F1221; Thermo Fisher Scientific) in Opti-MEM (11058-021 ...
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bioRxiv - Microbiology 2022Quote: ... 6-Diamidino-2-Phenylindole (DAPI, Ex 360 nm and Em 460 nm, Thermo Fisher, D1306) or (SYBR Green I ...
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bioRxiv - Cancer Biology 2019Quote: ... 5(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; CA-DCF-DA; (C400, ThermoFisher Scientific)) at a stock concentration of 20 mM in DMSO was diluted in DMEM without phenol red to a concentration of 40 μM ...
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bioRxiv - Developmental Biology 2020Quote: ... Discs were then incubated with 2.5 μg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in PBS + 0.1% Tween-20 for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Neuroscience 2022Quote: ... A nuclear counterstaining was performed using 4ʹ,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) in TBS for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2019Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 2 h with occasional agitation ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
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bioRxiv - Bioengineering 2023Quote: PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Immunology 2022Quote: ... The sorted cells were then incubated with Totalseq-C antibodies (1:50 dilution) on ice for 30 min, washed 3 times (by default, top up with 2% FBS (Gibco) in DPBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; Invitrogen, s911) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: Cultured DRG neurons were loaded with 4 μM Fura-2 AM (Life Technologies) in culture medium at 37°C for at least 60 minutes before use ...
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bioRxiv - Cell Biology 2021Quote: LX-2 and TWNT-4 human HSCs were lysed in TRIzol (Life Technologies) and total RNA was purified following the supplied protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 mL of 2 mM disuccinimidyl glutarate (DSG,Life Technologies, #20593, Carlsbad, CA) prepared in 1X PBS was added per 1 × 107 cells to the conical tube and the solution was mixed thoroughly by pipetting to remove clumps ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM MgCl2) and 4 μM of the phosphobinding protein (PBP) (Life Technologies) to detect the inorganic phosphate (Pi ...
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bioRxiv - Immunology 2020Quote: ... P2X7a 451L or P2X7a 451P were loaded with 2 μM Fluo-4 (Invitrogen) for 20 min at 4°C and 10 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... XEN cells were passaged every 2–4 days using TrypLE Express (Thermo Fisher) up to passage 20.
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bioRxiv - Cell Biology 2022Quote: ... 9 μl of carboxyl latex bead (4% w/v, 2 μm; Invitrogen, C37278), and 2 μl of Concanavalin A (1 mg/ml ...
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bioRxiv - Physiology 2023Quote: Freshly isolated cardiomyocytes were loaded with 2 μM Fluo-4-AM (Thermo Fisher) for 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... A pCR 4-TOPO or pCR- XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts) with an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain was used as a positive control ...