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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Low-cost drug discovery with engineered E. coli reveals an anti-mycobacterial activity of benazeprilbioRxiv - Synthetic Biology 2021Quote: ... cooled to 4 °C and lysed with 10 mL B-PER reagent (ThermoFisher 78248) and a standard EDTA-free protease inhibitor cocktail (Merck 11873580001).
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... and 6 μl of the 2X Luna Universal One-Step Reaction Mix was subjected to one-step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded at 3 μl/min for 6 min onto a 2 cm × 75 μm C18 trap column (Acclaim Pepmap 100, 3 μm, 300 Å, Thermo Scientific) in loading buffer (0.5% v/v formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Microbiology 2020Quote: ... colonies were stained using 500 µl of GC medium containing 3 µmol/l Syto 9 (Thermo Fisher Scientific) and 4.5 µmol/l propidium iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
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bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Cancer Biology 2019Quote: ... Blots were blocked for one hour with 3% (weight/volume) bovine serum albumin (Fisher Scientific) and incubated overnight at 4° C with a rabbit monoclonal antibody to RHAMM [EPR4055] antibody at 1:1,000 dilution (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... 2 M thiourea and 4% CHAPS (Thermo Fisher Scientific). Each combined replicate of samples was loaded onto a QIAshredder spin column (QIAGEN ...
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bioRxiv - Neuroscience 2021Quote: ... flash frozen in 2-methylbutane (Fisher Scientific, 03551-4), and kept at −80°C until sliced on a cryostat (Leica ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mL of cold 4% PFA (Thermo Scientific, 28908) in RNAse-free PBS (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 4 mM 2-Deoxy-D-glucose (2DG; ACROS Organics), 0.5 μM Thapsigargin (Tg ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotech) or the AttuneNxT acoustic focusing cytometer (4 lasers, 14 parameters, Thermo-Fisher Scientific). Data were processed using FlowJo software (ver.9.9 or 10.7 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed 3 times for 4 min with TBST and treated with Pierce ECL Western Blotting Substrate (Thermo Scientific) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Genomics 2020Quote: ... where 2–3 mm of the terminal ends of 3–4 filaments from the left side each fish were collected and placed in RNAlater (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... an Agilent LC-MS system consisting of a 1100 HPLC and a 1946D single quadrupole ESI mass spectrometer equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific) or 2 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... and then rinsed 3 times in sterile water and 4 times in Dulbecco’s Phosphate-Buffered Salines (DPBS) (ThermoFisher Scientific, Waltham, MA) [4] ...
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bioRxiv - Biophysics 2021Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subjected through high pH reverse phase fractionation into 3 or 4 fractions and all fractions were run independently on an Orbitrap Fusion (Thermo Scientific) mass spectrometer coupled to an Acquity M-Class nanoLC (Waters Corporation) ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: Cultured neurons at 19-21 DIV were incubated with 3 μM of the calcium dye Fluo-4 AM (Thermo Scientific, Sweden) in DMSO(Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: ... RAG was reconstituted in RAG1-deficient v-Abl cells via retroviral infection of cells with the pMSCV-RAG1-IRES-Bsr and pMSCV-FLAG-RAG2-GFP vectors followed by 3-4 days of Blasticidin (ThermoFisher, #R21001) selection to enrich for cells with virus integration.
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Bioengineering 2020Quote: ... The reaction was monitored using an Agilent G1946D LC/ESI-MS system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Bioengineering 2020Quote: ... All samples were analyzed using an Agilent 1100 HPLC system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... adult cardiomyocytes isolated from 3-4 month old mice were washed in PBS containing protease and phosphatase inhibitor cocktail (PIC; Thermo Scientific) and then fixed in 1% formaldehyde at 25°C for 8 min for cross-linking and followed by quenching by 0.125 M glycine at 25°C for 8 min as previously described ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were fixed with 4% paraformaldehyde (PFA) pH 7.4 at room temperature for 10 min and washed 3 times in PBS (Gibco Life Technologies,10010) for 5 min each ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were centrifuged at full speed for 3 min before loading on NuPage 4%–12% Bis-Tris Gels (Invitrogen, Carlsbad, CA). Gels were run at 140V for 55 min ...
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bioRxiv - Genomics 2021Quote: ... 3 and 4 and PABPC1 (ON-TARGETplus SMARTpool) or a scramble control (ON-TARGETplus SMARTpool) were performed in Optimem (Life Technologies), using Oligofectamine (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: Nucleotide sugars were separated using Inertsil ODS-4 250 × 4.6 mm column with a particle size 3 μm (GL Science) and Vanquish Flex HPLC system (Thermo Fisher Scientific), with 100 mM potassium phosphate buffer pH 6.4 containing 8 mM tetrabutylammonium hydrogen sulphate as buffer A and 70 % solution of buffer A in 30 % acetonitrile as buffer B ...
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bioRxiv - Cell Biology 2020Quote: ... A pool of 4 siRNAs targeting mouse SNAP-47 and control Luciferase siRNA (Target Sequence: 5’-CGTACGCGGAATACTTCGA-3’) (Dharmacon, Thermofisher Scientific) were electroporated along with GFP into E15.5 cortical neurons (2 µg GFP + 50 pmol siRNAs) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 - 4 ml aliquots of the resulting 10,000xg supernatants were added up with 3-2.5 ml 1X PBS (Gibco, Carlsbad, CA, USA) to reach a final volume of 6.5 ml and were transferred on top of a 60% - 10% iodixanol gradient (Progen Biotechnik GmbH ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... After 3 hours a fraction of the bacteria was fixed by the addition of 4% methanol-free paraformaldehyde (PFA, Thermo Scientific) for 10 min at room temperature and the reaction was quenched by the addition of 0.3 M glycine ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... 3 hours later cultures were washed and fixed with 4% paraformaldehyde (Electron Microscopy Solutions) in phosphate-buffered saline (PBS; Thermo Fisher) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: HSC cells atop ECM hydrogels subjected to the different treatments for 3 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...