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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... BODIPYTM-C12 500/510-C1 (4,4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid; Invitrogen) or BODIPYTM-C12 558/568 (4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid ...
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bioRxiv - Biochemistry 2024Quote: ... at a cell density of 3 to 5 × 106 cells/mL in 30 mL of Expi293 Expression Medium (ThermoFisher) in a humidified atmosphere containing 8% CO2 and shaking at 125 rpm ...
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bioRxiv - Cancer Biology 2024Quote: LDHC expression was quantified using specific 5′FAM-3′MGB Taqman gene expression primer/probe sets (Hs00255650_m1, Applied Biosystems). PD-L1 ...
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bioRxiv - Bioengineering 2024Quote: ... Lipid films were rehydrated in 5 mL C4F10 saturated PBS and DiD (1,1’-dioctadecyl 3,3,3’,3’-tetramethylindodicarbocyanine perchlorate; D307, Thermo Fisher Scientific) lipid dye was added when fluorescent MBs were desired ...
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bioRxiv - Bioengineering 2024Quote: ... organoids were washed 3 times for 5-min each using PBS and mounted on glass microscope slides (Fisher Scientific). 90 μm Polybead Microspheres (Polyscience ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... ethyl ester (TMRE) (1 nM; ThermoFisher Scientific) in order to obtain simultaneous metabolic measurements ...
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bioRxiv - Bioengineering 2020Quote: ... TMRE (Tetramethylrhodamine Ethyl Ester, Life Technologies/ThermoFisher) was diluted to a final concentration of 75 μM in sterile PBS according to our previously established protocols 45 ...
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bioRxiv - Bioengineering 2020Quote: ... TMRE (Tetramethylrhodamine Ethyl Ester, Life Technologies/ThermoFisher) was diluted to a final concentration of 75 μM in sterile PBS according to our previously established protocols 45 ...
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bioRxiv - Zoology 2021Quote: Tetramethylrhodamine ethyl ester (TMRE, ThermoFisher Scientific, T669) and CellEvent Caspase-3/7 Green ReadyProbes Reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M N-ethyl maleimide (ThermoFisher, #23030) and protease inhibitor (Cell Signalling Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed with cold PBS and biotinylated with 0.5 mg/ml sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) while gently shaking for 45 mins on ice ...
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bioRxiv - Immunology 2021Quote: ... iBMDMs were incubated for 3 h in 50 μM of the mitochondrial uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP; ThermoFisher, M20036). Control samples included ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... supplemented with 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine (DiI)-labelled acetylated-LDL (15µg/ml, Thermo Fischer) or DiI-labelled LDL (15 µg/ml, Thermo Fisher) and incubated at 37°C for 2 hours ...
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bioRxiv - Systems Biology 2021Quote: ... The Caspase-3 assay was performed on retina sections following the protocol described above (anti-caspase 3 (Fisher Scientific, 15889738) 1:500) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA biotinylation at the 3′ end was performed using the Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific, US) in accordance with the manufacturer’s instructions with some modifications ...
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bioRxiv - Biophysics 2022Quote: ... Texas Red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanloamine (TR-DHPE) and Oregon Green-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (OG-DHPE) were purchased from Thermo Fisher. PLL-PEG and PLL-PEG-biotin were purchased from SuSoS AG ...
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bioRxiv - Biophysics 2022Quote: ... Cryo-EM grids were imaged using SerialEM44 with a 3×3 image shift collection (with calibrated correction for image shift induced beam tilt) on a Titan Krios (ThermoFisher) equipped with a K3 camera and a Bioquantum energy filter (Gatan ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was end labelled at the 3’ end with biotin using the Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher). RNA quantity was assayed by running an RNA 6000 Nano chip on a 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2020Quote: mRNA associated with 1 to 3 ribosomes “Light polysomes” and mRNA associated with more than 3 ribosomes “Heavy polysomes” were extracted using TRIzol LS (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: To generate construct drg1 [prab-3∷GCaMP6m∷NLS∷unc-54 3’UTR] we performed a 4-way Gateway recombination reaction using LR Clonase II (Invitrogen). We recombined pDEST II with the following entry clones ...
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bioRxiv - Cancer Biology 2021Quote: ... Lipophilic dye DiL (DilC18(3) (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate) at 1mM stock in ethanol (Invitrogen; Carlsbad, CA, USA) was used to label nanoparticles to observe in vitro delivery ...
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bioRxiv - Microbiology 2022Quote: ... all used viruses were propagated to passage 3 on Calu-3 (ATCC HTB-55) cells in Advanced DMEM/F12 (Gibco), supplemented with HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded with the Fluorescent Dye-Based Rhod-3 AM following the manufactures’ instructions (Rhod-3 Calcium Imaging Kit, Cat.No. R10145; ThermoFisher scientific). Loading ...
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bioRxiv - Bioengineering 2024Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Cat. A638729) were purchased from Aladdin (Shanghai). KLH (Cat. 77600) was purchased from ThermoFisher. Peptide synthesis was conducted by Genscript (Nanjing ...
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bioRxiv - Neuroscience 2023Quote: ... a 5040 amperometric cell and a Hypersil Gold C18 analytical column (3 μm, 100 × 3 mm; Thermo Fisher Scientific, USA). The mobile phase consisted of 0.1 M KH2PO4 buffer at pH 3.8 ...
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bioRxiv - Genomics 2023Quote: ... A single-cell suspension from PBMC from each patient was quantified and analyzed for viability using the Cell counter 3 (Countess 3, Invitrogen) and then loaded onto the 10X Genomics Chromium Single Cell Controller for isolation of single cells (10X Genomics) ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal antibodies were diluted to 30 μg/mL and then serially diluted 1:3 to a final concentration of 1.52x10-3 μg/mL in PBST supplemented with 1% non-fat milk (Life Technologies). Following blocking ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed with 0.3% TX-100/PBS 3 times and then incubated with secondary antibody goat anti-rabbit IgG AlexaFluor 555 (A21528, Invitrogen) at room temperature for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (triethylammonium Salt) (Bodipy-PE: #D3800) was purchased from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2024Quote: ... OVCAR-3 and res OVCAR-3 cells were maintained in RPMI-1640 medium (AL162A; HiMedia) supplemented with 20% FBS (10270; Gibco), while the COV362 and res COV362 cells were cultured in DMEM (AL007A ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Bioengineering 2021Quote: ... Medium was changed every 2-3 days and cell passages were carried out using a TrypLE Express solution (Gibco). Human TERT-immortalized gingival fibroblasts (hTERT-HGF ...
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bioRxiv - Neuroscience 2020Quote: ... pooled and purified cDNA libraries with mean sizes of 550-600 bp and concentrations of 2-3 ng/µl (measured with Qubit 2.0, Invitrogen) were sequenced on a HiSeq 2500 Illumina system with single-end 50 bp read lengths ...
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bioRxiv - Cell Biology 2021Quote: ... 2 and 3 (100 pmol for each) or control siRNA were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen). 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco) supplemented with 5% New Born Calf Serum (NBCS ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 μg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 µg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Cell Biology 2021Quote: ... under 5% CO2 at 37°C in humidifying conditions and passaged every 2-3 days using 0.25% Trypsin-EDTA (25200056, Gibco). Cells were routinely tested for mycoplasma ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... A total of 3 µg of RNA was DNAse treated using 2 units of Turbo DNase I enzyme (Invitrogen). A total of 1 µg of DNAse-treated RNA was reverse transcribed using Superscript III reverse transcriptase (Invitrogen/Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability was determined by the MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Invitrogen, Waltham, MA) reduction assay ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... conditioned media was replaced by a 1.2 mM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per condition were plated in 96 U-shape well plates (CELLSTAR, Kremsmünster) and rested for 2-3 h in RPMI (Gibco), supplemented with 10% FBS ...