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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... MitoProbe TMRM (tetramethylrhodamine methyl ester) kit for flow cytometry (Invitrogen, # M20036) was used as per manufacturer’s instructions to measure the membrane potential ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... slides were then incubated simultaneously with two different secondary antibodies diluted in PBS/0.1% Tween 20/3% BSA for 1 h at RT to amplify the signal (goat anti-mouse Alexa Fluor 488 (Thermo Fisher, A11001; 1:250) and donkey anti-goat Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... FAZ was visualized via anti-FAZ1 antibody L3B2 (antigenic to Tb927.4.3740, 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Cancer Biology 2024Quote: ... diluted to a final concentration of 1,500 µg ml−1 and 3 µl of 100 mg ml−1 collagenase IV (Thermo Fisher Scientific, cat. no. NC9836075) diluted to a final concentration of 100 µg ml−1.
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Then the cells were stained with a solution of 4 mM calcein AM (TRC, #C125400) and 4 mM ethidium homodimer-1 (Thermo Fisher, #L3224) dissolved in PBS to identify live and dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle overnight at 4 °C (Alexa flor 488 goat anti mouse, Invitrogen A11001, 1:1000; Alexa flor 546 goat anti rabbit, Invitrogen A11035, 1:500). After 3 washes for 30 minutes in PBS ...
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bioRxiv - Immunology 2021Quote: ... The phage/serum mixtures were rotated overnight at 4°C after which 40 μL of a 1:1 Protein A/G coated magnetic bead mixture (Invitrogen, #10002D and #10004D) was added to each well and rotated for an additional 4 hours at 4°C to capture all IgG ...
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bioRxiv - Cell Biology 2020Quote: ... then placed on an agarose pad (4% agarose in sterile water mixed 1:1 with 10%FBS in DMEM (Gibco, Cat. No.11965), and with doxycyline (2 μg/ml)) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with anti-KLK10 (BiossUSA bs-2531R, 1:100) primary antibody overnight at 4°C followed by Alexa Fluor-647 (ThermoFisher Scientific, 1:500) secondary antibody for 2hrs at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were preincubated in 3% Bovine serum albumin-PBS at room temperature for 1 h and incubated overnight at 4°C with primary antibodies against CC-ORF (rabbit anti-GFP, polyclonal, Invitrogen A11122, 1:1000). The sections were then incubated with the corresponding fluorescein-conjugated secondary antibodies (Alexa Fluor 488 anti-rabbit ...
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bioRxiv - Developmental Biology 2023Quote: ... -GFAP (1:500, SynapticSystems) -Aquaporin-4 (1:200, Alomone Labs) as well as with Click-iT™ EdU Cell Proliferation Kit (Thermo Fisher Scientific) according to manufactures protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Cell Biology 2024Quote: ... then stained for 1 h at 4°C in PBS with 1:1000 Alexa-647 conjugated M1 mouse anti-FLAG antibody (Invitrogen, Cat #MA1-91878). Cells were moved to flow cytometry (BD FACS Canto2) ...
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bioRxiv - Cell Biology 2024Quote: For nuclei and F-actin staining were visualized with 4’,6-diamidino-2-phenylindole (DAPI) (#D523, Dojindo; 1:1000 dilution) and phalloidin Alexa Fluor plus 405-conjugated (#A30104, Invitrogen; 1:400 dilution), phalloidin ATTO 565-conjugated (#94072 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated overnight at 4°C with the following primary antibodies: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), anti-TIAR (BD Transduction ...
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bioRxiv - Cell Biology 2024Quote: ... 3D or in suspension as described above were incubated with cell-impermeable 4 µM ethidium homodimer 1 (EthD-1; Invitrogen catalogue no. E1169) and 10 µg/ml cell-permeable Hoechst (Thermo Fisher catalogue no ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were grown exponentially in an incubator at 37°C under 5% CO2 and subcultured at ratios of 1:2-1:10 every 2 to 4 days using 0.05% trypsin-EDTA (Thermo Fisher Scientific, Cat# 25300120).
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bioRxiv - Neuroscience 2024Quote: ... This was followed by primary antibody staining overnight at 4°C (Tuj1 (1:1000, made on site) and Sox10 (1:150, ThermoFisher, catalog PA5-37890). The next day ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C (for P. miniata) or 20C (for P. regularis) in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Genetics 2022Quote: ... and washed 3-4 times with fresh 10% RNA later solution (Thermo Fisher Scientific). After the last wash ...
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bioRxiv - Zoology 2022Quote: ... The excised tissues were then placed (3-4 pieces) on gelatin (Attachment Factor, ThermoFisher)-coated dish (35 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... 3-4 μl of samples were applied onto grids using a Vitrobot (Thermo Fisher). Grids were blotted with “595” filter papers (Ted Pella ...
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bioRxiv - Genetics 2021Quote: Calu-3 cells were mock transfected with 4 μL of lipofectamine 3000 (ThermoFisher Scientific) in Opti-MEM (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was replaced after 3-4 hr with neurobasal medium (Gibco, Cat# 21103049) supplemented with 2% v/v B27 (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with DMEM and fixed with 4% paraformaldehyde (Thermo Fisher) at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... with 10% FBS and split every 3-4 days using TrypLE Express (Thermo Scientific). Low-passage ...
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bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days using 0.25% Trypsin-EDTA (Fisher Scientific) incubated at 37°C for 3-5 minutes to detach cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 h at 4°C and then with protein G magnetic beads (ThermoFisher) at 4°C for an additional 2 h to obtain immunoprecipitated RNA fragments ...
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bioRxiv - Genomics 2024Quote: ... were treated 3-4 times with 50 U of T4 PNK (Thermo Fisher Scientific) and 100 U of λ-exonuclease (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Fluorescent labeling of antibodies was done with DyLight 488 or DyLight 647 Amine-Reactive Dye (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: NCR044.1 was labeled with DyLight550 amine reactive fluorescent dye following the manufacturer’s instructions (Thermo Scientific, Waltham, MA). Confocal microscopy on a Leica SP8-X was performed to monitor uptake of the fluorescently labeled NCR044.1 peptide as described previously54 with minor modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were then labeled with either the amine-based TMT 16-plex reagents (Thermo Fisher Scientific) for 1 hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... which were then labeled with an amine-reactive TMTsixplex Isobaric Mass Tagging Kit (90064B, Thermo Fisher Scientific) and fractionated with reversed phase chromatography at pH 12 (Xbridge column ...
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bioRxiv - Immunology 2021Quote: ... Control and target cell populations were labeled with fluorescent amine binding dyes CFSE (Molecular Probes, Eugene, OR) and e450 (eBiosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μg of peptides were reacted with the corresponding amine-based TMT six-plex reagents (Thermo Fisher) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Then 0.1 μl of 10% biotinylated dextran amine (BDA, 10,000 MW, ThermoFisher Scientific, Waltham, MA, United States) or 4% FluroGold (FG ...
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bioRxiv - Biochemistry 2023Quote: ... Amine-specific crosslinking of histones within the complex was done with bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific). 20-μl reactions containing complex in (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 [28] and were labeled using amine-reactive 16-plex TMT kits (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: NCR13_PFV1 and PFV2 were labeled with DyLight550 amine reactive fluorescent dye following the manufacturer’s instructions (Thermo Scientific). B ...
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bioRxiv - Immunology 2021Quote: ... After 3 consecutive washes anti-rabbit Alexa Fluor-568 conjugated antibodies (1:500; Thermo Fisher Scientific) were applied for 2 h before counterstaining with DAPI.
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...