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Citations for Thermo Fisher :
1251 - 1300 of 7068 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
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bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... the His-tagged protein was purified using the ProBond Purification System (Thermo Fisher Scientific) and the eluted protein was dialyzed against PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the media was removed from the HEK293 CLK (NL-CLK1, CLK2-NL or CLK4-NL) transfected cells and replaced with 85 μL OPTI-MEM media (Gibco). NanoBRET Tracer 5 (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were purchased from ATCC and cultivated in DMEM medium supplemented with 10% fetal calf serum (Thermo Fisher Scientific). Cells were maintained at 37°C ...
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bioRxiv - Neuroscience 2020Quote: The HEK293 cells (CRL-1573, ATCC, USA) were kept in the Dulbecco’s Modified Eagle Medium (High Glucose)-DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum-FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Phoenix cells (National Gene Vector Biorepository, Indianapolis, IN) were transfected with each construct using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfection of 2N3T-Tip60 or the corresponding control empty vector was carried out for 48 hours into HEK293 cells using Lipofectamine 2000 transfection reagent (Invitrogen); UV irradiation was carried out at a dose of 15mJ/cm2 using Stratagene Stratalinker 1800 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-C2-IQGAP1 and pcDNA3/RH-p122RhoGAP/DLC-1 plasmids were transfected into HEK293 cells using Lipofectamine 3000 (Invitrogen, MA).
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bioRxiv - Microbiology 2021Quote: ... Plasmids encoding the SARS-COV-2 spike glycoprotein or its mutated variants were cloned into a G418 resistance gene containing backbone and transfected into HEK293 cells using Lipofectamine 2000 (Thermofisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... The inter-molecular (oligomer) and intra-molecular (monomer-conformation) aSyn FRET biosensors were generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with GFP-aSyn and aSyn-RFP (1:8 DNA plasmid concentration ratio ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 and Ptk2 cell lines were cultured in DMEM (high glucose, pyruvate) supplemented with 10 % FBS and antibiotic-antimycotic (Invitrogen) and passaged routinely ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... pX330 plasmid harboring a p53-specific gRNA (Supplementary Table 1) was transfected into HEK293 and HCT116 cells using Lipofectamine 2000 (Invitrogen). Two days later ...
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bioRxiv - Molecular Biology 2022Quote: Inducible HEK293 cell lines expressing mt-ELP3 were generated by using the Flp-In T-REx Core Kit from Invitrogen. Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the IFN-β–Luc was co-transfected with pRL-TK in wild type and 4EHP-KO HEK293 cells using Lipofectamine 2000 according to the manufacturer′s protocol (Invitrogen). 24 h after transfection ...
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bioRxiv - Immunology 2022Quote: Binding to P-selectin of the A48 and D48 variants of PTX3 from HEK293 cells was then assessed using 96 well Maxisorp plates (Nunc) coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein ...
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bioRxiv - Cell Biology 2019Quote: ... Stable tetracycline inducible Flp-In T-REx HEK293 cell lines were generated using the T-Rex system (Thermo Fisher Scientific) as previously described (Kang et al. ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5 μg psPAX2 packaging plasmid and 500 ng pMD2.G envelope plasmid were co-transfected in HEK293 FT cells in 10-cm dishes using Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293-B3GALT4 KO and HEK293-B3GALT4-PIGZ DKO cells stably expressing HFGF-CD59 were treated with 0.5 unit/mL PI-PLC (Thermo Scientific) in 5 mL of PI-PLC buffer (Opti-MEM containing 10 mM HEPES-NaOH (pH 7.4) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: African green monkey kidney epithelial cells (Vero, ATCC) and HEK293 T cells (ATCC) were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco Invitrogen) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 Flp-In T-Rex cells were maintained in DMEM supplemented with 10% tetracyclin-free fetal bovine serum (Gibco, 10082139) and 1% penicillin/streptomycin (Gibco ...