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Citations for Thermo Fisher :
1251 - 1300 of 1533 citations for Measles Virus Nucleoprotein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
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The direction of local adaptation on pathogen exploitation rates reverses with environmental contextbioRxiv - Evolutionary Biology 2023Quote: ... we extract virus from the pooled samples from each assay grid using a sterile pellet pestle (Fisher Scientific, U.S.A.) to manually burst larvae and release viral occlusion bodies ...
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bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Cell Biology 2021Quote: HEK293T (ATCC® CRL-3216™) and S1/S2 DKO HEK293 cells were maintained in cell culture in 1X DMEM (41965-039, Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... five-fold serial dilutions of each virus stocks were prepared in a separate 96-well plate in Opti-MEM (Gibco). Medium in the 96-well plate was removed and the cells were washed with 100 µL of warm PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... the plate was checked for confluence and the cells were infected with serial dilution of virus in OptiPro serum free media (Thermofisher) with 1% L-Glutamin (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μl of RNAse-Out and 1 μl of the Moloney Murine Leukemia Virus reverse transcriptase enzyme (MML-V, Invitrogen). Quantitative PCR was carried out in a total of 15μl consisting of 3μl cDNA ...
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bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
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bioRxiv - Developmental Biology 2019Quote: Pure cultures of 10 × 104 prostate stromal cells seeded on twelve well plates were transduced using Cytotune 2.0 Sendai Virus reprogramming vectors (KOS, c-Myc and Klf4, Life Technologies) at a multiplicity of infection of 5-5-3 (KOS MOI=5 ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Cell Biology 2019Quote: ... and HY03 were reprogrammed using the Sendai virus and the CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), and display all the PSC features ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM with glutamine (Life Technologies) with 0.7% IgG-free BSA (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... Untransduced cells and cells treated with the serially diluted virus were both cultured in the presence of 2 µg/ml puromycin (Invitrogen). The lentiviral titer was quantified by counting the number of living cells at the time when all of the untransduced cells died as previously described26.
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bioRxiv - Biochemistry 2020Quote: ... The plasmid was linearized with XbaI and in vitro transcribed into the genomic RNA of DENV-Luc virus using the T7 Megascript Kit (Ambion) in the presence of m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The virus particles were then harvested 24 to 48 hours later by passing through a 0.45 µm syringe filter (Thermo Scientific). Viruses were mixed with an equal volume of fresh media supplemented with 10% FBS and polybrene was added at a final concentration of 5 µg/mL to increase infection efficiency ...
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bioRxiv - Neuroscience 2021Quote: ... Cell reprogramming was performed using the Sendai virus system with SOX2/OCT4/KLF4/MYC (CytoTune-iPS Reprogramming Kit; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... by the acid guanidinium/phenol/chloroform method using ISOGEN (Nippon Gene) was converted to cDNA using Moloney murine leukemia virus reverse transcription (Invitrogen) and oligo-dT primers ...
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bioRxiv - Bioengineering 2021Quote: ... a pure DI244 seed virus at MODIP 1E–3 and 20 U/mL trypsin (5000 U/mL in PBS; 27250018, Gibco) were added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... ROMT2 and ROMT3 were transferred into the binary destination vector pB2GW7 including Cauliflower mosaic virus (CaMV) 35S promoter through Gateway LR clonase recombination (Invitrogen). Together with ROMT1 and STS48 constructs (Schmidlin et al. ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... VeroE6 cells were used for live virus titration of biological samples and were maintained in DMEM (#11965092, Thermo Scientific, USA) with 10% FBS.
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bioRxiv - Microbiology 2021Quote: ... Five μl RNA (150 µl elution volume) was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...