Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 100ng of FLAG-Ubiquitin-expressing plasmid and 200ng of HA-KDM5-epxressing plasmid using the Effectene II (Invitrogen) kit according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were co-transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP and different pKH011-puro guide plasmids together with the piggyBAC transposase plasmid via electroporation with the Neon Transfection System (ThermoFisher). Transfected cells were selected and maintained with 6 μg/mL Blasticidin and 6 μg/mL Puromycin ...
-
bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
-
bioRxiv - Biophysics 2023Quote: ... and 200 ng NICD-expressing plasmid (or empty pcDNA3.1(+) negative control plasmid) using Lipofectamine LTX with PLUS reagent (Life Technologies). Cells were lysed 44-48 hours post-transfection by adding 250 μL Passive Lysis Buffer (Promega ...
-
bioRxiv - Biochemistry 2023Quote: ... the cells were co-transfected with 200 ng of prime editor plasmid and 100 ng of pegRNA plasmid using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.8 μg each PBK/TECTAWT and PBK/TECTARS expression plasmids and 0.1 μg PCAGGS/TMPRSS2 plasmid were transfected to each well using 400 μl OptiMEM (Invitrogen, 31985070) and 2 μl Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: SUM159 cells were transfected with the donor plasmid and the PX459 plasmid containing the sgRNA targeting sequence using Lipofectamine 3000 (Invitrogen). Five to seven days after transfection ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 50 ng of sgRNA plasmid and 50 ng of nuclease or base editor-expressing plasmid were added to 5 µL of Opti-MEM (Gibco). 0.2 µL of TransIT®-2020 transfection reagent (Mirus Bio ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μg of specific plasmid, 0.5 μg of hybrid transposase plasmid (Yusa et al., 2011) and Lipofectamine 2000 transfection reagent (Invitrogen™) with a 1:2.5 DNA:transfection reagent ratio.
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected with 2 μg of dual-nuclease plasmid and 0.66 μg of transposase plasmid using Lipofectamine 3000 reagent (Thermo Fisher) and positive clones were selected based on their resistance to puromycin ...
-
bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
-
bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
-
bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
-
bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
-
bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
-
bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
-
bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
-
bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
-
bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
-
bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
-
bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
-
bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
-
bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were introduced using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were diluted in 1 ml OptiMem (Gibco) and 20 μl of PEI (1 mg/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed with Lipofectamine 2000 (ThermoFisher) as recommended by the manufacturer ...
-
bioRxiv - Genetics 2021Quote: ... plasmids were purified using the Purelink kit (Thermofisher). Unless noted otherwise ...
-
bioRxiv - Genomics 2020Quote: ... plasmids using lipofectamine plus 2000 (Thermo Fisher Scientific). After 5h ...
-
bioRxiv - Molecular Biology 2020Quote: ... This episomal plasmid contains a GateWay™ (Invitrogen) recombineering cassette that allows the cloning and expression of fungal effectors without a signal peptide (SP) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transfected with Lipofectamine 3000 (Invitrogen, L3000015) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... plasmids using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Cells were incubated for 24 to 48 hours in the transfection solution ...
-
bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine3000 (ThermoFisher Cat #L3000001), while in Figure 1d and Supplementary Figure 2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine2000 (ThermoFisher Cat #11668030), following the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2020Quote: Minigene plasmids were transfected using Lipofectamin 3000 (Invitrogen) with reverse transfection protocol for 24 hours followed by cells harvesting by trypsin-EDTA (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: Plasmid transfections were performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen). Selection was performed with G-418 (Sigma) ...
-
bioRxiv - Developmental Biology 2019Quote: ... plasmids using the Neon Transfection System (Life Technologies). Forty-eight hours post-transfection ...
-
bioRxiv - Biochemistry 2020Quote: ... Plasmids were generated using Gateway cloning (Life technologies) using the Drosophila Gateway Vector Collection (DGVC ...
-
bioRxiv - Synthetic Biology 2020Quote: Plasmid DNAs were purchased from GeneArt (ThermoFisher scientific), General biosystems Inc ...
-
bioRxiv - Physiology 2019Quote: ... Indicated plasmids were transfected with Lipofectamine-2000 (Invitrogen) (for 36-42 hours) ...
-
bioRxiv - Cell Biology 2019Quote: Plasmid transfection was performed with Lipofectamine2000 (Thermo Fisher) according to manufacturer’s protocol ...