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Citations for Thermo Fisher :
1251 - 1300 of 3328 citations for HVEM Rhesus Macaque HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... 293 and 2934×4 knock-out clones were generated by transfection (lipofectamine 2000, Invitrogen) of CRISPR–Cas9-expressing knockout plasmids (MAVS ...
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bioRxiv - Immunology 2019Quote: ... 293F suspension cells were grown in Freestyle 293 expression medium (Life Technologies 12338-018) on a shaker incubator ...
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bioRxiv - Biochemistry 2019Quote: The Ca2+-flux assay was performed using Flip-In 293 cell line (Life Technologies) stably expressing full-length T1r2a ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37 °C at 135 rpm with 8% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using ExpiFectamine™ 293 Transfection Kit according to manufacturer’s directions (Thermo Fisher Scientific, USA). The cells were then cultured for 4-5 days and removed by centrifugation at 800×g for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... using polyethylenimine in Freestyle 293F cells cultured in 293 FreeStyle media (Thermo Fisher Scientific). Where indicated ...
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bioRxiv - Microbiology 2022Quote: ... then re-suspended in 30 mls FreeStyle 293 Expression Medium (no. 12338018; Thermo Fisher). An aliquot of cells was taken for counting and the cells were spun again at 300 xg for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: Expi293F cells were transfected with the purified plasmids using ExpiFectamine 293 transfection kit (Invitrogen), where the fusion proteins were expressed and harvested at 96 hrs post-transfection under 8% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the medium was replaced with 30 ml FreeStyle 293 Expression Medium (Thermo Fisher Scientific). After 3 days ...
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bioRxiv - Molecular Biology 2019Quote: ... pMDG2 in 293-LX packaging cell line using lipofectamine LTX (15338500, Thermo Fisher Scientific). Viral supernatants were harvested 48 h post transfection and concentrated using Amicon Ultra-15 centrifugal filter units (UFC910024 ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Biochemistry 2019Quote: ... were maintained in suspension in protein-free chemically-defined FreeStyle 293 Expression Medium (Invitrogen / Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Neuroscience 2021Quote: A stable cell line was generated from Flp-In T-Rex 293 cells (Invitrogen), according to the manufacturer recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with the vectors using the ExpiFectamine 293 Transfection kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: Stable cell lines were produced using Flp-In™-293 cells (Thermo Fisher Scientific), authenticated by STR profiling (FTA barcode ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-Rex 293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific, RRID:CVCL_U427) cells were cultured in high-glucose ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells were cultured using 66% FreeStyle 293 Expression / 33% Expi293 Expression medium (ThermoFisher) and grown in polycarbonate baffled shaking flasks at 37 °C and 8% CO2 while shaking at 120 rpm ...
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bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using TransIT®-293 Transfection Reagent (Mirusbio) and Opti-MEM (Thermofisher). After 6 to 12 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... CE0-3 SOSIPs were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). The modified CH505 transmitted founder Env SOSIPs were chimeras containing the CH505 gp120 and BG505 gp41 domains ...
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bioRxiv - Cell Biology 2022Quote: ... were grown in suspension culture in FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific) to density of 4 x 106 cells/ml and harvested by centrifugation (500 x g ...
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bioRxiv - Microbiology 2024Quote: ... HEK293F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37°C at 135 rpm with 8% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific, Waltham, MA, USA). Recombinant huBPI ...
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bioRxiv - Synthetic Biology 2023Quote: ... culture media was replaced by Freestyle 293 Expression Medium (Fisher Scientific 12-338-026), supplemented with 50UI/mL penicillin/streptomycin (Corning 30001CI) ...
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bioRxiv - Systems Biology 2023Quote: ... were used to generate tetracycline-inducible stable Flp-InTM-293 T-REx cells (Invitrogen). A 6-well plate was co-transfected with 0.5 µg of the pEXPR plasmid and 1 µg of the pOG44 Flp-recombinase expression vector (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Flp-In™ T-REx™ 293 Cell Lines (Thermo Fisher, catalog number: R78007) expressing Snrpb endogenously from a single locus were generated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with expression constructs using the Expifectamine™ 293 Transfection Kit (Thermo Fisher) following the manufacturer’s guideline ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a slow filter paper (#293; 1–2 µm Sartorius, Thermo Fisher Scientific), and then used to determine electrical conductivity (EC) ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced using standard methods in FreeStyle™ 293-F Cells (Invitrogen) (91 ...
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bioRxiv - Biophysics 2023Quote: ... HEK293F cells were cultured in 30 ml of Freestyle 293 serum-free medium (Gibco). For transfection of HEK293F cells ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells were cultured in Freestyle™ 293 expression medium (Gibco).
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bioRxiv - Molecular Biology 2024Quote: ... RRID:CVCL_0291) and Flp-In-T-REx-293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... were maintained in Life Technologies FreeStyle 293 Expression Medium with GlutaMAX (Thermo Fisher Scientific). Murine T cell hybridomas (TCHs ...
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bioRxiv - Cancer Biology 2021Quote: ... FC-1242, and FC-1245)(Engle et al., 2019) were cultured in 10% FBS (Biowest) and 1% Anti-Anti (Gibco) in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and enzyme activity was quenched by addition of FCS followed complete DMEM medium (supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 10000 U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of Fc control and CLEC18A-Fc fusion were incubated with AlexaFluor647 rabbit anti-mouse and goat anti-rabbit IgG (Invitrogen) at a molar ratio of 4:2:1 in array PBS and preincubated on ice for 10 mins at a total volume of 65 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Bioengineering 2021Quote: ... and GibcoTM fetal calf serum (FCS) (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... supplemented with 10% fetal calf serum FCS (Gibco), 10 mM Hepes pH 7.2 (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5% fetal calf serum (FCS; Gibco), 5% human serum AB (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... HBSS with 2 % FCS (Gibco, cat no. 10500064) was used for the whole procedure ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% foetal calf serum (FCS) (Gibco) and 1% GlutaMAX (200mM ...
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bioRxiv - Immunology 2021Quote: ... containing 10% fetal calf serum (FCS; Gibco, Germany), penicillin (100 U/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with fetal calf serum (10%, FCS, Invitrogen) and Antibiotic-Antimycotic (1% ...