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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... M-100126-01-0005), rat Esrrg (Dharmacon, M-100212-01-0005), rat E2f1 (Dharmacon, M-102754-00-0005) or scramble control siRNA (ThermoFisher, 4390843), using the Viromer BLUE Transfection Reagent (Lipocalyx ...
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies were washed and SlowFade antifade kit (Thermofisher, S2828) was used to mount the coverslips for Confocal imaging by Zeiss LSM510 (Germany ...
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bioRxiv - Microbiology 2020Quote: ... the AbC Total Antibody Compensation Bead kit (Molecular Probes, A10497) was used upon incubation with both primary and secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... was conjugated to AlexaFluor647 using the Antibody Labeling Kit (ThermoFisher). The concentration decoy reagent was quantified using a NanoDrop ...
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bioRxiv - Bioengineering 2023Quote: ... A Site-click Qdot 655 antibody labeling kit (Invitrogen, S10453) was used to conjugate 125 μg of Tau-5 to dibenzocyclooctyne (DIBO ...
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bioRxiv - Biochemistry 2024Quote: Protein cages were labeled with AF647 Antibody Labeling Kits (Invitrogen). Over 500 µg of protein cage was incubated per reaction for 1 hour at 25°C with periodic inversion ...
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bioRxiv - Cell Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor Goat anti-Rat 488 Invitrogen #A-11006, 1:1000 and Alexa Fluor Goat anti-Rabbit 594 Invitrogen #A-11012, 1:1000). Coverslips were mounted on slides with Fluoromount-G mounting media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were washed in PBS-T and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed three times in PBS-T and were incubated for 2 hr with the secondary antibodies Alexa Fluor® 488 or 546 Goat Anti-Mouse/Rat/Rabbit IgG (Life Technologies; 1:200) and then with DAPI (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 1 hour at room temperature with goat anti-rat Alexa-Fluor 488 conjugate antibody (Molecular Probe, ThermoFisher Scientific Waltham, MA, USA) diluted 1:500 in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed and incubated with 1 µg/ml Donkey anti-Rat polyclonal Dylight 488-conjugated antibody (Thermo Fisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and the secondary antibodies solution (1:75 dilutions of Alexa Fluor 488 goat anti-rat and Alexa Fluor 546 goat anti-mouse [Molecular probes, A11006 & A11030, respectively]), always washing with PBS-T in between ...
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bioRxiv - Neuroscience 2019Quote: ... at 4°C overnight, rinsed in PBS (3 times, 5 min each) and amplified by Alexa Fluor-488 donkey anti-rat antibody (Life Technologies, A21208, 1:600). Sections were mounted with Fluoromount containing DRAQ-5 (1:1000).
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bioRxiv - Plant Biology 2021Quote: ... the slides were incubated with secondary goat anti-rabbit or goat anti-rat antibodies conjugated with Alexa Fluor 488 or Alexa 555 (Thermo Fisher, diluted 1:100) in PBS containing 0.01 % acetylated BSA at 37 °C for 2h ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated for two hours at room temperature with goat anti-Rat IgG (H+L) Alexa Fluor 488 secondary antibody (ThermoFisher Scientific, diluted 1:200). Antibodies were diluted in 1×PBS with 5% BSA and incubated in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific, A11006, 1:1000) for CD44 and Alexa Fluor-594 anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were washed 3 times with PBS for 5 min each and incubated with secondary antibodies anti-mouse Alexa fluor 594 and anti-rat Alexa fluor 488 (1:250, Invitrogen #A11005 and #A11006, respectively) in blocking buffer for 1h at RT in a dark humid chamber ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed twice with PBS and incubated with a goat anti-rat Alexa Fluor 488 antibody (ThermoFisher, A-11006, 1:1000 dilution), DAPI (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were washed 3 times with PBS for 5 min each and incubated with secondary antibodies anti-mouse Alexa fluor 594 and anti-rat Alexa fluor 488 (1:250, Invitrogen #A11032 and #A11006, respectively) in blocking buffer for 1h at RT in a dark humid chamber ...
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bioRxiv - Genomics 2023Quote: ... sections were incubated for 1 hour with a cocktail of Alexa Fluor-conjugated secondary antibodies at 1:200 (Goat anti-Rabbit IgG AF488 – Thermo Fisher Scientific A32731; Goat anti-Rat IgG AF647 – Thermo Fisher Scientific A21247), followed by counterstaining with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2 h at room temperature in the appropriate combinations of Alexa 488-594-647-conjugated anti-mouse/rabbit/guinea pig or rat IgG secondary antibodies (1:500; Molecular Probes, Eugene, OR, USA). Sections were also stained with a nuclear stain DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were then incubated at 4°C overnight in 1.5% Normal Donkey Serum in PBS-T with rat anti-PDGFR-β antibody (Affymetrix, 14-1402, 1:500), goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1 ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted IL-10 using the IL-10 Rat ELISA Kit (Invitrogen, catalog # BMS629). The resulting values were normalized based on the cell viability ...
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bioRxiv - Immunology 2021Quote: Antibody-conjugated beads were prepared according to the company’s protocol (Invitrogen™ Dynabeads™ Antibody Coupling Kit) at 10 µg antibody for 1 mg beads ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was then labeled with the Alex Fluor 546 antibody labeling kit (Thermo Fisher Scientific, A20183) according to the manufacturer’s instructions (SI Fig ...
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bioRxiv - Microbiology 2020Quote: ... the antibody directly conjugated to Alexa Fluor 647 using an Alexa Fluor 647 Antibody Labelling Kit (ThermoFisher).
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bioRxiv - Immunology 2022Quote: Quantum Dot (QD) antibody coupling was done using SiteClick Qdot 655 Antibody Labeling Kit (Molecular Probes, S10453) according to manufactures instructions ...
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bioRxiv - Genetics 2023Quote: ... antibodies were labeled directly using Alexa Fluor 488 and Alexa Fluor 568 antibody labeling kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the anti-CD63 antibody was labelled using Zip Alexa Fluor 488 rapid antibody labelling kit (ThermoFisher, USA) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006, Goat anti Rat 647 Thermo Fisher Scientific a21247 ...
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bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).
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bioRxiv - Biochemistry 2020Quote: ... AlexaFluor 594–conjugated anti-rat (1:250, Molecular Probes) and AlexaFluor 488–conjugated anti-mouse (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... and goat α-rat (62-6520, Invitrogen, 1:5000). Proteins were detected using West Pico Chemiluminescent Substrate for 20 seconds (34578 ...
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bioRxiv - Cell Biology 2020Quote: ... and goat-α-rat 488 (Thermo Fisher A-11006) at 1:3,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: Donkey anti rat Alexa Fluor 594 (A21209, Life Technologies) and donkey anti mouse Alexa Fluor 647 (A31571 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 488/680 (Invitrogen, CA, USA), anti-mouse Alexa Fluor 488/800 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and anti-rat Alexa Fluor 546 (1:500, Invitrogen). The incubations were performed in the dark in a humidified chamber ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-rat (goat polyclonal, Thermo Fisher, A-21434, RRID:AB_2535855); Alexa Fluor 594 ...
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bioRxiv - Immunology 2022Quote: ... rat IgG2a kappa isotype control (1:400, Thermo Scientific), armenian hamster IgG isotype control (1:400 ...
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bioRxiv - Immunology 2022Quote: ... rat IgG2a isotype control (1:400, Thermo Fisher Scientific), rat IgG2b kappa Isotype control (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... AF-647 goat anti-rat (Invitrogen #A21247; 1:500), Fluorescein (FITC)-conjugated donkey anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2022Quote: ... goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific), rabbit anti-mouse-Cy3 1:400 (Dianova) ...
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bioRxiv - Neuroscience 2021Quote: ... and monoclonal rat anti-mCherry (#M11217, ThermoFisher, RRID: AB_2536611) primary antibodies in 0.1M Tris-buffered saline containing 0.5% Roche Blocking Reagent (#11096176001 ...