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Citations for Thermo Fisher :
1251 - 1300 of 5346 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... Human embryonic kidney 293 cells (HEK293) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD14 (11-0149-42, Thermo Fisher Scientific, 1:100), anti-human CD117 known also as cKIT (12-1178-42 ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Microbiology 2021Quote: ... or a type IV DQTM collagen conjugate from human placenta (Invitrogen). Reactions were prepared in technical triplicate or quadruplicate by mixing 20 µL of substrate at 0.25 mg/mL with 80 µL of reaction buffer and 100 µL of bacterial suspension ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human mammary epithelial cells (HuMECs) were provided by Thermo Fisher Scientific and cultured according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: Cultured primary human airway epithelial cells were washed with PBS (Gibco), fixed with 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Microarray analysis was performed with the Human Gene 1.0ST Array (Affymetrix). Protein expression was analyzed by Simple Western (ProteinSimple) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Immunology 2021Quote: ... efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific, MA, USA), FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... We quantified expression using the IgG4 Human ELISA kit (Invitrogen BMS2095) with IgG4 standards provided in the kit ...
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bioRxiv - Genetics 2021Quote: Human TERT cDNA39 was cloned into the gateway pDONR221 plasmid (Invitrogen). TERT variants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... human testicular cells were suspended in MEMα medium (Thermo Fisher Scientific) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Human ESCs were cryopreserved in PSC Cryomedium (Gibco, Cat. No. A2644601). The culturing medium was supplemented with 1X RevitaCell (100X ...
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bioRxiv - Cell Biology 2022Quote: ... Human NGFR siRNA or negative control siRNA were obtained from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... human neurons were transfected with Lipofectamine 3000 (#L3000015, Thermo Fisher Scientific) and 1.6 μg of plasmid DNA at 40 - 50 DIV ...
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bioRxiv - Neuroscience 2021Quote: ... Primary human and NHP microglia were cultured in DMEM/F12 (Gibco) supplemented with 10% defined fetal bovine serum (HyClone) ...
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bioRxiv - Genomics 2020Quote: ... 20x human TERT TaqMan™ Copy Number Reference Assay (ThermoFisher 4403316) as internal reference control ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Cancer Biology 2019Quote: ... human 36B4 RT 5′-CCCATTCTATCATCAACGGGTACAA-3′) using Superscript III (Thermo Fisher) at 55 °C for 1 h ...
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bioRxiv - Bioengineering 2019Quote: Human primary dermal fibroblasts derived from adult female forearm (ThermoFisher, C0135C) were cultured in DMEM-High Glucose with Glutamax (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: The human C10orf76 gene (Uniprot Q5T2E6) was synthesized by GeneArt (Thermofisher). c10orf76 and PI4KB (Uniprot Q9UBF8-2 ...
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bioRxiv - Genomics 2019Quote: Proliferating human myoblasts were cultured in F10 medium (Gibco/Life Technologies) supplemented with 20% fetal bovine serum (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: Proliferating human myoblasts were cultured in F10 medium (Gibco/Life Technologies) supplemented with 20% fetal bovine serum (Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... human plasma fibronectin (#2334564) and phytohemagglutinin (#10576015) from Invitrogen (Carlsbad, CA); anti-human CD3ε (OKT3 ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2.5 ng/mL of recombinant human IL-2 (Thermofisher, PHC0027). T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher ...
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bioRxiv - Molecular Biology 2019Quote: ... RNAs were hybridized on GeneChip Human Exon 1.0 ST Arrays (Affymetrix), and scanned following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 500 μL cRPMI with human T-Activator CD3/CD28 Dynabeads (Gibco) were added to each well at a ratio of 2 cells ...
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bioRxiv - Immunology 2019Quote: ... were coated with either goat anti-human Ig (10μg/ml, Invitrogen) or purified GPIIbIIIa (5µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL of human recombinant epidermal growth factor (Invitrogen PHG0313), 10 ng/mL of basic fibroblast growth factor (Invitrogen PHG0263 ...
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bioRxiv - Immunology 2021Quote: ... head/tail larvae or human cell pellets with TRIzol reagent (Invitrogen) following the manufacturer’s instructions and treated with DNase I ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco, #PHC0027), 2 mM L-glutamine (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... Primary adult human endothelial keratinocytes (HEKa) cells were purchased from ThermoFisher. HEKa cells were cultivated in EpiLife™ Medium ...
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bioRxiv - Bioengineering 2021Quote: ... including Human PD-L1 and PD-1 were purchased from Invitrogen. Human LAG-3 and Na+K+ATPase antibodies were purchased from Cell Signaling Technology and Santa Cruz Biotechnology Inc ...