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Citations for Thermo Fisher :
1251 - 1300 of 7012 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: HEK293 cells were obtained from ATCC (CRL-1573) and grown in DMEM (high glucose, L-glutamine, no sodium pyruvate) (ThermoFisher), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Neuroscience 2024Quote: ... ASAP3 construct was cloned in pcDNA3.1/Puro-CAG plasmid backbone and used to transfect HEK293 cells with Lipofectamine 3000 (ThermoFisher, US) using 400 ng DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa (ATCC CCL-2) and HEK293 (ATCC CRL-1573) cell lines were maintained in Dulbecco’s Modified Eagle Medium (Invitrogen 11965092) supplemented with 10% fetal bovine serum (Invitrogen 16140071) ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... To generate stable expression cell lines Flp-In T-Rex HEK293 were grown in 100 μg/ml Zeocin (Invitrogen, R25001) for at least a week before transfection ...
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bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting constructs were integrated into HEK293 Flp-In T-REx cells using the Flp-In technology (Thermo Fisher Scientific). HEK293 Flp-In T-REx were seeded in 6-well plates and co-transfected the following day with 200 ng bait-BirA*-FLAG construct and 2 μg pOG44 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2024Quote: HEK293 (XX female) cells and HepG2 cells were cultured in high glucose Dulbecco’s modified eagle medium (DMEM) (41965, Thermo Fisher) and supplemented with 10% fetal bovine serum (10270 ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Genomics 2020Quote: For Hi-C experiments knockdown of CLAMP was validated using the Western Breeze kit (Invitrogen). Antibodies used for detection were a previously described custom rabbit antiCLAMP (1:1,000 ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS, Thermo Fisher Scientific, Loughborough, UK) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Molecular Biology 2019Quote: ... The Jpx E1-E3 isoform was cloned into pEF1/V5-His vector (Invitrogen Cat# V92020), which contains an EF-1α promoter for mammalian expression and a T7 promoter for in vitro transcription ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved 14His-SUMO and His-Ulp1 were removed by affinity to Ni-NTA Agarose (Invitrogen). The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... under the control of an inducible pBAD promoter (pBAD/His A, Thermofisher, Catalog number 43001) (Supplementary Table 5) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were subcloned into the pAc5.1/V5-His A vector (Invitrogen, V4110-20) with a GFP tag sequence at its 3’ end terminus ...
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bioRxiv - Microbiology 2019Quote: ... with 30% L929 supernatant and 20% fetal bovine serum (HI-FBS, Thermo Scientific, Waltham, MA) for 48 hours at 37°C under 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: His-V5-uL4-CGN mRNAs were obtained using a mMESSAGE mMACHINE T7 kit (Invitrogen #AM1344) using linear DNA templates ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... His-tagged RRs were purified from clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) or HisPur Cobalt resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The his-tag was cleaved using AcTEV protease according to manufacturer’s instructions (Invitrogen, Carlsbad, CA), and the his-tagged protease and noncleaved uSpike protein were removed by binding to Ni-NTA resin ...
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bioRxiv - Cell Biology 2019Quote: ... the above mutants are sub-cloned into pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020) with EcoRI and XhoI (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with Alexa Fluor 488–conjugated 6x-His Tag monoclonal antibody (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2019Quote: ... was added to multiplexed product with Hi-Di™ Formamide (Thermo Fisher Scientific, Waltham, MA) to aid in denaturing ...
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bioRxiv - Microbiology 2020Quote: ... All pBAD inducible plasmids generated in this work were derived from pBAD/His A (Invitrogen). pJNS12 is pBAD-gfp-linker-virB flanked by NcoI and HindIII restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... Curli fibers were detected via direct fluorescent immunostaining with an α-6X-His antibody (Invitrogen) conjugated to a fluorescent dye ...