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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 4 Tert Butyldimethylsilyl Oxy Methyl 2 Iodothiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
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bioRxiv - Cell Biology 2022Quote: ... MBL) and an additional 2 h at 4°C with 20 μL of a slurry of IgG-conjugated Dynabeads (Invitrogen). After washing the beads five times with IP buffer ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and grown to an OD600 range of 2-4 at 30°C in a deep-well 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Bioengineering 2023Quote: PAAm gels prepared on coverslips were transferred to multiwell plates before covering with 0.2 mg/mL sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH) (Thermo Fisher) solution ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Genetics 2023Quote: ... Protein was separated on a 4–12% Bis-Tris gel and transferred using iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... TUBB4A and MUC5AC overnight at 4 °C and then incubated with Alexa 488-F(ab’)2 fragment of goat anti-mouse IgG (Invitrogen) for 2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Intracellular NO production was assessed during reoxygenation by live-cell staining with the NO-sensitive fluorescent dye 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein (DAF-FM) diacetate (Invitrogen). iPSC-ECs were stained with 1 μM DAF-FM diacetate for 10 minutes at 37°C ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in 15-cm Petri dishes were grown to 60-70% confluency and then transfected with 4 µg of the UTR library of DNA templates (2 µg for each UTR library) using lipofectamine 3000 reagent (Invitrogen). Fourteen hours after transfection ...
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... and A-253 cells were serially diluted and seeded at three different dilutions (2, 4 and 128 cells/well) with 50 µL serum-free DMEM/F12 medium (Gibco) supplemented with 10 ng/ml human EGF (R & D systems) ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Microbiology 2024Quote: ... Transfectants were selected with either 4 nM WR99210 (Jacobus Pharmaceuticals; pSLI) or 2 μg/μl blasticidin S (Life Technologies; pSLI2). SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al. ...
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bioRxiv - Plant Biology 2024Quote: ... CrGAP1-4 and CrGDI1-2 were PCR amplified from leaf cDNAs using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) and gene-specific oligonucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... Gradients were ultracentrifuged at 36,000 rpm for 2 hours at 4°C using a TH-641 rotor (Thermo Fisher Scientific) and fractionated on a Piston Fractionator (BioComp).
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bioRxiv - Neuroscience 2024Quote: ... retinas were incubated with secondary antibodies at 4°C for another 2-3 days: goat anti-chicken antibody conjugated to Alexa Fluor 488 (1:1000; A11039, Invitrogen), goat anti-rabbit 555 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Microbiology 2024Quote: ... for 30 min followed by staining of host cell nuclei with 4′,6-diamidino-2-phenylindole (DAPI, 5 μg/ml final conc.; ThermoFisher) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... all wells were incubated for 5 minutes in PBS containing 0.1% 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, D1306) to counterstain nuclei ...
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bioRxiv - Systems Biology 2024Quote: ... A subset of APCs was fixed for 30 minutes in 4% PFA and stained with 2 μM BODIPY (Thermo Fisher) and 20 μM Hoechst (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... and 3 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) anti-CD28 (MA110172, Thermo Fisher), and cultured in the pre-coated plate for 3 days ...
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bioRxiv - Immunology 2024Quote: ... 2 to 4×106 CD8+ T cells were lysed in the lysis buffer provided and passed through a homogenizer (Invitrogen) before RNA purification following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Bioengineering 2024Quote: ... the sections were rinsed in PBS and the nuclei were counterstained and mounted with Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI; #S36939, Invitrogen). The slides were imaged at a 4X magnification using EVOS M7000 (ThermoFisher Scientific™ ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg of protein (or all of the sample for the MDF multiplexing experiment across 2 gels) was loaded into a NuPAGE 4∼12% Bis-Tris 1.5 mm gel (Invitrogen #NP0335) and gel electrophoresis performed ...
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bioRxiv - Neuroscience 2024Quote: ... before incubation with DAPI (4’,6-diamidino-2-phenylindole, dilactate, 0.1 µg ml−1 final concentration in PB, Invitrogen D3571) in 0.1 M PB for 10–20 mins at RT ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were plated on 25 mm coverslips coated with PLL (0.01%) and Collagen IV (4 µg/ml) and loaded with 10 µM Fura-2 AM (Thermo Fisher) with 0.02% Pluronic F-127 (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA labeling was performed using PBS containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (1:10000) (Invitrogen, D1306). After 3 additional washes with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were washed three times with permeabilization buffer and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen #D1306). The primary antibodies used included rabbit Anti-Brachyury (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed again with PBST and incubated with 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes, 1:2000 dilution) and phalloidin-tetramethyl rhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... and 2x the concentration of all the other supplements used in R10 medium (see above) and either 2% or 4% of UltraPure Agarose (Invitrogen) dissolved in one part water to achieve different agarose stiffnesses ...
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bioRxiv - Plant Biology 2023Quote: ... To detect sinapoylmalate and intact indole-3-methyl glucosinolate (I3M) contents, an AcclaimTM RSLC120 C18 column (100 mm x 3 mm, 2.2 µm) (ThermoFisher Scientific, MA) was used in conjunction with mobile phases consisting of solvent A (0.1% formic acid (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... All chemical inhibitors were diluted in DMSO and were: 3-O-Methyl-Sphingomyelin (SMPD3 inhibitor, Enzo Life technologies, BML-SL225-0005), FAM14 inhibitor (FAK inhibitor ...
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bioRxiv - Cell Biology 2024Quote: The fluorescent cholesterol probe was tracked by incubating the cells with a final concentration of 20 μg/ml of TopFluor® Cholesterol (810255, Avanti) and 200 μg/ml of methyl-beta- cyclodextrin (C4555-1G, ThermoFisher) in PBS for 2 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... the suspending fluid was 0.6% w/v or 0.7% w/v methyl cellulose (Thermo Scientific, molecular weight 454.513 g mol−1) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... By measuring the absorbance of the supernatant at maximum wavelength for the Reactive Violet 5R (λmax = 558nm) and Methyl red (λmax = 420 nm) using Spectronic 20D+ (Thermo Scientific) the decolorization percentage was calculated using below equation,
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bioRxiv - Cell Biology 2024Quote: ... Analysis of total 5-methyl-2ʹ-deoxycytidine (5-mdC) concentrations was performed using a Q exactive mass spectrometer (Thermo Fisher Scientific), equipped with an electrospray ionization source (H-ESI II Probe ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential was measured using tetramethylrhodamine methyl ester (TMRM) from the MitoProbe™ TMRM Assay Kit (Thermo Scientific™). Cells were harvested and incubated with TMRM at 37°C for 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated at 60 °C for 5 min and then 2.5 μL of 200 mM MMTS (methyl methanethiosulfonate, Thermo Fisher, #23011) was added ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...