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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were centrifuged at 16,000g for 5 minutes at −4°C in a Sorvall Legend Micro 17R microcentrifuge (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were boiled at 95°C for 5 min and loaded on SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). Efficiency of ligand coupling to the Ad capsid (% total hexon protein coupled ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4 °C in TBST-5% BSA—a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 100°C for 5 min and then separated on NuPAGE Novex 4-12% Bis-Tris midi gels (Invitrogen) using NuPAGE MES SDS Running buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
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bioRxiv - Neuroscience 2022Quote: ... samples were mixed using either standard SDS Lämmli sample buffer and heated at 80°C for 5 min prior to loading onto gels (Novex Nupage 4-12% Bis- Tris gels, 1mm, 15 well, Invitrogen) and ran with Novex Nupage MES-SDS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Then the samples were heated at 95°C for 5 min and loaded onto a pre-cast Polyacrylamide 4-12% gradient gel (Bolt, Invitrogen). The proteins were then transferred onto a nitrocellulose membrane and then blocked with 10% Blotto (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in primary antibody in 5% milk and TBS-T overnight at 4°C at the following dilutions: 1:1000 rabbit αFLAG polyclonal (Invitrogen) and 1:1000 rabbit αHsp70 polyclonal (Abcam) ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Physiology 2022Quote: HEK293-H cells were maintained in suspension at 37°C with 5% CO2 in CD293 medium supplemented with 4 mM GlutaMAX (Invitrogen). For imaging and electrophysiology ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041 ...
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bioRxiv - Bioengineering 2024Quote: ... MOLT-4 cells were maintained at 37°C with 5% CO2 in suspension culture in a complete growth medium (Gibco’s RPMI 1640 with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated at 95°C during 5 minutes and separated by SDS-PAGE using 4-12% Bis-Tris gels (Invitrogen), stained for 1 hour using BioSafe-Coomassie stain (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μL of sample was applied and the grid was blotted for 5 seconds at 4 °C and 95% relative humidity then plunge-frozen with liquid ethane using a Vitrobot (ThermoFisher). Samples were clipped and screened by Glacios transmission electron microscope before 5,276 movies were collected on a Titan Krios transmission electron microscope at the Center for Electron Microscopy and Analysis (CEMAS ...
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bioRxiv - Genetics 2024Quote: ... Samples were boiled at 95°C for 5 min before loading (60 µg) on 4-12% Bis-Tris gel (ThermoFisher) and ran with 1X MES buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were suspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged (13,000 × g for 5 min at 4°C) and supernatants are precleared with 10 μl protein G beads (Invitrogen; 1004D) at 4°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged at 500 g for 5 minutes at 4°C and red blood cells were hemolyzed using ACK buffer (Gibco). White blood cells were then incubated with 1:1000 Fc Block (AbLab ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... blocked in 5% donkey serum and incubated overnight at 4°C with a rabbit anti-Rbm20 antibody (PA5-53068, Invitrogen) at 0.5 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected (5 minutes, 1,000 rpm, 4°C) and RNA was isolated using the PureLink™ RNA Mini Kit (Ambion). RNA concentration was measured using NanoDrop 8000 (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... heat-denatured at 95°C for 5 minutes and loaded onto a 4-12% gradient premade 1.0 mM Bis-Tris gel (Life Technologies) with molecular weight standard SeeBlue Plus2 (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Sorted iNKT cells were cultured in cRPMI in 96-well plates that were previously coated for 12 h at 4 °C with 5 μg/mL of anti-CD3ε (clone 145-2C11, Invitrogen) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... pH = 7.6) followed by an overnight incubation at 4°C with 1:1500 anti-mouse Tau-5 antibody (Invitrogen #AHB0042) in superblock blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were boiled at 95°C for 5 min and separated by SDS-PAGE electrophoresis using 4-12% Bis-Tris 80 NuPage gels (Invitrogen) at 180V for 70 min ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heated for 5 min at 95°C and loaded on a 4-12% Tris-Glycine gel (XP04122BOX, Invitrogen). The transfer onto a PVDF membrane (IB24001 ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Microbiology 2023Quote: ... Substrate 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS; Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2020Quote: ... Asaia1 (5’-AGC ACC AGT TTC CCG ATG TTA T-3’) and Asaia2 (5’-GAA ATA CCC ATC TCT GGA TA-3’) labeled with Alexa Fluor® 555 (Invitrogen). Tissues were visualized using Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera.
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bioRxiv - Cancer Biology 2020Quote: ... and scrambled control mimics (sense 5’ - mCmArUmArUmUrGmCrGmCrGmUrAmUrAmGrUmCrGC - 3’; antisense5’ - /5Phos/rGrCrGrArCrUrArUrArCrGrCrGrCrArArUrArUmGmG rU - 3’; IDT) were reverse transfected at 2nM using Lipofectamine RNAiMax (Life Technologies) according to manufacturer guidelines.
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bioRxiv - Physiology 2021Quote: ... RNA targeting exon 3 of Ctns (gRNA-ex3 5’-ATCTTTCCAGAATCAACCGTCGG-3’) was produced using the Precision gRNA Synthesis Kit (Thermo Scientific). Online tools RGEN (http://www.rgenome.net/cas-designer/ ...
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bioRxiv - Immunology 2023Quote: ... and 500 nM each of the forward primer and reverse primer (Table 3) with the following cycling conditions on either QuantStudio 3 or 5 Real-Time PCR systems (Applied Biosystems): 95°C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was incubated overnight at 4 °C with beads (Invitrogen) previously conjugated with 1 µg of mouse monoclonal anti-human TRAP1 (sc-73604 ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C and the supernatant added to Ni-NTA Agarose (Invitrogen) equilibrated in His-tag buffer ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...