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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Dionex Ionpac AG11-HC b (2 mm x 50 mm, 4 μm particle size, ThermoFisher Scientific), was placed before the separation column ...
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bioRxiv - Physiology 2020Quote: ... brain tissue was removed and rapidly frozen using 2-methylbutane (Thermo Fisher Scientific, Cat# O3551-4) at -50°C to prevent tissue cracking ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were separated in a SDS-PAGE (2-4% gradient gel, NuPage™; ThermoFisher Scientific) and blotted to a PVDF membrane (300 mA ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were further ruptured using a syringe and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were sonicated and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and then flash-frozen in dry-ice-chilled 2-methylbutane (−40 °C) (#03551-4, Fisher Scientific). Regions of interest were collected from these slices using a 1 mm biopsy micropunch (#15110-10 ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Cat. no. 15630-080,) and 1% penicillinstreptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of plasmid DNA was mixed with 4 µL of Lipofectamine 2000 (ThermoFisher Scientific #11668019) in 300 µL of Opti-MEM without any additives ...
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bioRxiv - Genetics 2024Quote: ... media was half-changed every 4 days with 2 µg/mL Natural Mouse Laminin (ThermoFisher Scientifics). Recordings were performed starting on day 115 using a Maestro Edge MEA system and AxIS Software Spontaneous Neural Configuration and Viability Module ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitation was performed at 4°C for 2 h in micro-spin column (Thermo Scientific, 89879), and the beads were washed three times with lysis buffer and two times with Mass Spec buffer (0.1 M Tris-HCl in mass grade water ...
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bioRxiv - Cell Biology 2022Quote: ... typically 2-20 μg samples were loaded on 4–12% Bis-tris gradient gels (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... isolated FDB fibers were loaded with 4 µM fura-2 AM (Thermo Fisher, Carlsbad, CA, USA) in Ringer’s solution at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants were first precleared for 2 h at 4°C using Protein G Dynabeads (Invitrogen). Precleared samples were immunoprecipitated with anti-H3K9me3 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4 mL trituration buffer (Hibernate-A with 2% B27; ThermoFisher Scientific) and 2 mM sodium pyruvate (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Embryos older than 4 days were treated with 2 u/µl T1 RNAse (Thermo Fisher Scientific) after probe washing in order to reduce background ...
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bioRxiv - Immunology 2024Quote: ... These were transfected at a 1:2:4 w/w ratio using Lipofectamine 3000 (Invitrogen #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated at 4°C for 2 hours with Dynabeads Protein G (Invitrogen, 10003D) magnetic beads that bound anti-RNA polymerase II C-terminal domain antibody CTD4H8 (Abcam ...
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bioRxiv - Microbiology 2024Quote: We quantified round 2 PCR product concentration using a Qubit 4 Fluorometer (ThermoFisher, Ref. No. Q33238). We then pooled samples at masses (ng ...
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bioRxiv - Physiology 2024Quote: ... for 20 min at 4°C in PBS containing 2% fetal bovine serum (FBS) (10270, Gibco). Cells were then labeled with CD45 (25-0451-81 ...
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bioRxiv - Genomics 2024Quote: ... and lacking sodium pyruvate and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (McCoy‘s 5A, Gibco 16600082), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5 × Vilo reaction mix (Life Technologies, 11754), and 2 μl of 2 × Superscript III enzyme blend (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Neutrophils were loaded with Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...
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bioRxiv - Immunology 2023Quote: ... High 5 cells (BTI-Tn-5B1-4; Thermo Fisher Scientific) were infected with virus particles ...
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bioRxiv - Genetics 2024Quote: ... were passaged every 4-5 days using Accutase (Thermo Fisher) in the presence of 1uM Thiazovivin (THZ ...
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bioRxiv - Cell Biology 2024Quote: ... and passaged every 4-5 days with EDTA (ThermoFisher Scientific). Media change was performed every 24 hours (h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections were stained with hematoxylin and eosin (H&E) or mounted with 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (Thermo Fisher Scientific, Waltham, MA, USA). They were imaged with SLIDEVIEW VS200 Slide Scanner (Olympus ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco 15630080) and 1 % penicillin-streptomycin (PS ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were passaged every 3-4 days using Accutase (Life Technologies) and reseeded at 0.5×104 cells/mL on Geltrex-coated (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... followed by 3 mL of methanol (Fisher Scientific; Cat #A454-4), and 600 µL of 3% HCl (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and the [4– 3] destination vector (pCFJ201) using LR clonase (Invitrogen). Plasmids were inserted into the worm genome as a single copy using the MosSCI technique (Frøkjaer-Jensen et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and passaged every 3-4 days with 0.05% Trypsin-EDTA (Gibco), and after a few passages ...
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bioRxiv - Cell Biology 2022Quote: ... and passaged every 3-4 days with 0.25% Trypsin-EDTA (Gibco).
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bioRxiv - Cell Biology 2024Quote: ... Fast DiI (1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocyanine, 4-chlorobenzenesulphonate, D7756, Invitrogen) to back label parasympathetic preganglionic neurons (PPNs ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were passaged every 3-4 days with TrypLE (12604013, Gibco). Cells were authenticated by STR and tested for mycoplasma annually through Genetica Inc a subdivision of LabCorp.
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bioRxiv - Biophysics 2023Quote: ... with 3% (v/v) acetonitrile (Thermo Fisher, Cat. No. A996SK-4) and B was 100% acetonitrile ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...