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Citations for Thermo Fisher :
1201 - 1250 of 7910 citations for Recombinant Human RPE His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... recombinant murine EGF (50 ng/mL; Thermo Fisher Scientific), recombinant murine Noggin (100 ng/mL ...
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bioRxiv - Genetics 2021Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (#10777019, Thermo Fisher Scientific). All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L ...
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bioRxiv - Microbiology 2020Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies, USA) as described above ...
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bioRxiv - Plant Biology 2020Quote: ... 1 unit/μl RNaseOUT ™ Recombinant Ribonuclease Inhibitor (Invitrogen), and 20mM Tris-HCl/10% glycerol for 25 minutes in ice ...
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bioRxiv - Genetics 2019Quote: ... and 200 µg/mL recombinant proteinase K (Thermo Scientific). The mixture was incubated at 37°C for 30 min and then 95°C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with recombinant Protein G agarose (Invitrogen) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... ISG15 recombinant rabbit monoclonal antibody (1□00) (Invitrogen, 703132), and collagen I (1□100 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific), 100 ng/ml recombinant human IGF-1 (BioLegend) ...
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bioRxiv - Biophysics 2020Quote: Production of recombinant baculovirus followed the manufacturer’s protocol (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: Recombinant protein expression and purification FreeStyle 293F (ThermoFisher Scientific) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). The lysates were cleared by centrifugation at 200,000 ×g for 10 min at 4°C 3 times to remove the contamination of the lipid ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). 10% of the precipitates were analyzed by western blotting to check the protein immunoprecipitation efficiency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Genomics 2022Quote: ... 0.5 μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019) was then added to each well ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant EcoSSB and Sequenase© were purchased from Thermofisher. T7DNAp was purchased from NEB ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Microbiology 2023Quote: ... recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), and then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Microbiology 2023Quote: ... Recombinant protein A/G conjugated to HRP (Thermo Fisher) diluted 1:500 in TBST was added each well and the plates were incubated for 1 hour at 37°C then washed four times with TBST ...
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bioRxiv - Cell Biology 2022Quote: ... 60 units of RNaseOUT Recombinant RNase Inhibitor (Invitrogen, 10777019) was added to each sample and vortexed for 5 seconds ...
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bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
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bioRxiv - Cell Biology 2023Quote: ... 100U/mL RNAse OUT Recombinant Ribonuclear Inhibitor (ThermoFisher 10777019), and 1X complete EDTA-free Protease Inhibitor Cocktail (Sigma 11836170001)] ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl of Recombinant Protein G – Sepharose beads (Invitrogen) per condition were washed 3 times in IP buffer ...
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bioRxiv - Immunology 2023Quote: ... Recombinant mouse (rm)IFN-γ was purchased from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/ml recombinant EGF (PMG8041, Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL of RNaseOutTM Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant murine IL-13 (Catalogue #210-13, ThermoFisher Scientific) and/or human Gastrokine 3 (GKN3 ...
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bioRxiv - Immunology 2024Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies 10777019) suing a thermocycler ...
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bioRxiv - Cell Biology 2020Quote: ... 3×104 hTERT-RPE cells expressing mKO2-PACT were seeded into 8 well glass bottom dishes (Nunc™ Lab-Tek™ II Chambered Coverglass, Thermo Fisher Scientific) and cultured overnight ...
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bioRxiv - Cell Biology 2021Quote: The human epithelial cell line hTERT RPE-1 (ATCC-CRL4000) was grown at 37°C in 5% CO2 in DMEM/F12 (Thermo Fisher Scientific, Carlsbad, CA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: hTERT-RPE-1 p53−/− cells were cultured in DMEM (Thermo Fisher Cat No. 11965092 supplemented with 7.5% FBS, 1X Glutamax (Thermo Fisher Cat No. 35050061), 1X Non-Essential Amino Acids (Thermo Fisher Cat No ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2019Quote: ... and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher). The libraries were sequenced on an Ion Proton system (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hi-Q reagents and PI™ chip kit V3 (ThermoFisher, Waltham, USA) and the sequencing was performed for 300 flows ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...
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bioRxiv - Cancer Biology 2020Quote: ... respectively supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco). Co-cultures of JeKo-1/REC-1 with MS-5 and HS-5 at a 10:1 ratio was maintained under the same conditions as for MS-5 or HS-5 cells alone ...
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bioRxiv - Plant Biology 2021Quote: ... using the Ion PI Hi-Q OT2 200 Kit (ionTorrent, Life Technologies).
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bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
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bioRxiv - Genomics 2020Quote: All splicing reporters were cloned into pAC-5.1-V5-His (ThermoFisher Scientific) using compatible restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... and 9.8 μL of highly deionized Hi-DiTM formamide (Applied Biosystems, France). Samples were analyzed by Eurofins genomics (Ebersberg ...
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bioRxiv - Biophysics 2021Quote: ... The His-MBP tag was removed by cleavage with TEV protease (Invitrogen). Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 8 were initially cloned into pCDNA3.1/myc-His A (Invitrogen) and were later subcloned into pBJ5 vector using the NEBuilder HiFi DNA assembly kit (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... and ACE2 His constructs were produced in Expi293 cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All anCas genes were cloned into pBAD/His expression vector (ThermoFisher Scientific) and transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal mouse anti-6x-His antibodies (Epitope-tag-clone: HIS.H8, Thermo Fisher) were employed ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS; Gibco™), 1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... GBA was cloned into pcDNA3.1/myc-His A vector (Thermo Fisher Scientific) after digestion with EcoRI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT2 PCR Profiler Arrays (Qiagen) ...