Labshake search
Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for GCLC Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
-
bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
-
bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
-
bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
-
bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
-
bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
-
bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
-
bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
-
bioRxiv - Microbiology 2022Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
-
bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
-
bioRxiv - Molecular Biology 2024Quote: ... Recombinant adenoviruses expressing the GRK2 shRNA were produced using BLOCK-iT™ Adenoviral RNAi Expression System (Invitrogen) according to the manufacturer’s instruction.
-
bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
-
bioRxiv - Immunology 2024Quote: ... The concentration of the purified recombinant proteins was determined using the BCA protein assay kit (Thermo Scientific), with bovine serum albumin (BSA ...
-
bioRxiv - Biochemistry 2024Quote: ... The Dicer/TRBP enzyme was the mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
-
bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
-
bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
-
bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
-
bioRxiv - Immunology 2023Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) with bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
-
Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
-
bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for recombinant DNA cloning and Golden Gate Assembly were purchased from Thermo Scientific (Waltham, MA) and New England Biolabs (Ipswich ...
-
bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
-
bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
-
bioRxiv - Immunology 2024Quote: ... either the recombinant CT584 or purified Cm EB’S were solubilized in SDS sample buffer (Novex, Life Technologies) in the presence of reducing agent (10mM DTT) ...
-
bioRxiv - Cancer Biology 2019Quote: ... AF488-anti-rabbit (Invitrogen), AF594-anti-mouse (Invitrogen) ...
-
bioRxiv - Cancer Biology 2019Quote: ... AF594-anti-rabbit (Invitrogen), AF488-anti-rabbit (Invitrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... or rabbit (Invitrogen; 31460) secondary antibodies conjugated to HRP were used to detect primary antibodies.
-
bioRxiv - Molecular Biology 2019Quote: ... Isotype IgG rabbit (Invitrogen) or mouse (Santa Cruz ...
-
bioRxiv - Neuroscience 2020Quote: ... rabbit anti-FAM171A2 (ThermoFisher), mouse anti-Tenascin-R (R&D Systems) ...
-
bioRxiv - Microbiology 2021Quote: ... Donkey anti-rabbit (Invitrogen), Donkey anti-mouse (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... for rabbit (Thermo Fisher Scientific Cat# A-11034 ...
-
bioRxiv - Neuroscience 2021Quote: ... and rabbit IgG (Invitrogen) as a control ...
-
bioRxiv - Neuroscience 2020Quote: ... Rabbit IgG (#31235, Invitrogen) served as control ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit-antineun/fox3 (Invitrogen 702022 ...