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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 7H Benzocyclohepten 7 one 2 amino 5 6 8 9 tetrahydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... All sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher) before mounting.
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bioRxiv - Cell Biology 2024Quote: ... Cell nuclei were labeled with dapi (4 ’, 6-diamidino-2-phenylindole, Invitrogen, P36931). Images were obtained in the Zeiss LSM 800 Confocal Optical Microscope at Centro de Micro y Nanoscopía de Córdoba (CEMINCO-CONICET-UNC ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with 4’,6-diamdino-2-phenylindole (DAPI) (1:500, ThermoFisher, 62248) in 1X DPBS for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were stained with DAPI (40, 6-diamidino-2-phenylindole; 1:5000, Invitrogen). The slides were mounted with a mounting medium (S3023 ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer containing 4ʹ,6-diamidino-2-phenylindole (DAPI, 1:1000, Invitrogen). Sections were embedded in fructose–glycerol clearing solution Image analysis was performed using ImageJ and Imaris software.
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 kb RT-PCR products covering the S gene deletion were amplified from the viral RNA using the gene specific primers F9newF and F9newR (5’-TAAGGTTGGTGGTAATTATAATTACCTG-3’ and 5’-AAAATAGTTGGCATCATAAAGTAATGGG-3’) and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThemoFisher). A region spanning the deletion was sequenced using primers Wu_24_L and Wu_24_R (5’-TTGAACTTCTACATGCACCAGC-3’ and 5’-CCAGAAGTGATTGTACCCGC-3’).
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bioRxiv - Cell Biology 2024Quote: ... one well was loaded with 5 µg of input mitochondria previously diluted in 5 µL of 2x LDS dye (Thermo Fisher Scientific, 84788) with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell nuclei were counter stained with 4’,6’-diamidino-2-phenylindole dihy-drochloride (DAPI; 2 ng/ml; Molecular Probes) prior to mounting onto glass slides and then cover slipped with ProLong Gold (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/ml of fibroblast growth factor-2 (FGF-2) (all from Gibco). Cells were seeded at a density of 3200 cells/cm2 until they reached 90% confluency ...
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bioRxiv - Genetics 2020Quote: TaqMan copy number assays for CYP2D6 (assay IDs Hs04083572_cn and Hs00010001_cn for intron 2 and exon 9 respectively, Thermo Fisher Scientific) were run according to the manufacturer’s protocol on a ViiA7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Per2Luc mice and littermate wild-type control mice were euthanized by isoflurane 2-3 hours before lights off (ZT 9–ZT10) and tissues were immediately removed into ice-cold HBSS (Gibco). Coronal brain slices containing SCN (400-µm thickness ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... we prepared a 2-fold dilution series of a 1x “stimulation” solution containing 1x essential amino acids (Invitrogen, 11130051), 1x non-essential amino acids (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... templated and sequenced using either Ion 550™ Kit-Chef and Ion 550 Chip Kit as one 16-plex library pool or Ion 540™ Kit-Chef and Ion 540 Chip Kit as one 8-plex library pool (Thermo Fisher). Two independent sequencing analyses were performed on a panel of eight samples ...
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bioRxiv - Plant Biology 2020Quote: A total of 85 μg of protein per sample was subjected to one-dimensional SDS-PAGE using an 8-16% precise protein gradient gel (Thermo Fisher Scientific). Proteins were separated for 1 cm in the resolving gel and the entire lane was excised and cut into equal pieces ...
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bioRxiv - Biophysics 2023Quote: ... 150 μL of the diluted suspension was transferred into one well of a Nunc Lab-Tek II 8-well chambered coverslip (Thermo Scientific, USA) and the sample allowed to settle for 10 minutes to form a GUV monolayer at the bottom of the chamber ...
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bioRxiv - Biophysics 2023Quote: 150 μL of the washed vesicle suspension was transferred into one well of a Nunc Lab-Tek II 8-well chambered coverslip (Thermo Scientific, USA) and the sample allowed to settle for 10 minutes to form a GUV monolayer at the bottom of the chamber ...
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bioRxiv - Neuroscience 2023Quote: ... and then reconstituted directly in 50% acetonitrile in 100mM HEPES (pH 8) containing one of the TMT tags from the TMTpro 18-plex reagent (Thermo Fisher Scientific). The peptide-TMT mixture was incubated in a thermomixer for 1 h at 25°C and 600 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... A CFSE working solution was prepared by adding 8 µL of DMSO and 1 µL of Fast Green to one vial of CellTrace CFSE (CellTraceTM CFSE, Life Technologies, #C34554) for a final concentration of 10 mM ...
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bioRxiv - Bioengineering 2021Quote: ... 6-well plates were first coated with vitronectin (5 μg/mL, Thermo Fisher, USA) followed by a coating with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... 5–6 trophectodermal cells were biopsied and their ploidy was assessed by Thermo Fisher Scientific’s NGS technology ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (9 μL) were loaded onto PepMap 100 C8 trap column (5 mm × 300 μm i.d.; Thermo Fisher Scientific, Waltham, MA) at a flow rate of 40 μL/min of solvent A (0.1% TFA ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed in triplicates using 1 µl cDNA and 9 µl master mix consisting of 5 µl SYBR Green PCR Mix (4309155, Applied Biosystems), 3 µl H2O and 1 µl of forward and reverse primers (0.5 µM final concentration) ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Cell Biology 2022Quote: CHMP2AΔC-mutants containing residues 9 to 161 and CHMP3-mutants containing residues 9 to 183 were synthesized (ThermoFisher), subcloned in a pETM40 vector (PEPcore facility-EMBL Heidelberg ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Biophysics 2021Quote: ... Then 2 equivalents of fluorescein-5-maleimide (Invitrogen, #F150) was added and the mixture was incubated at room temperature for 20 min ...
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bioRxiv - Neuroscience 2019Quote: ... 2% yeast extract and 5% sucrose (#S3, Fisher Scientific), or 5% sucrose ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μmol EdU (5-ethynyl-2-deoxyuridine, Life Technologies) was added 30 min prior washing with PBS and fixation with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2022Quote: ... EdU (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific, cat# A10044) staining was performed by Click-it Alexa Fluor 647 imaging kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and 5 ng/ml IL-2 (Thermo Fisher Scientific), and then resuspended with 1 ml of virus per 6 x 106 cells ...
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bioRxiv - Cell Biology 2021Quote: 0.1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen, A10044) dissolved in PBS (50ul ...
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bioRxiv - Developmental Biology 2020Quote: 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044, Carlsbad, CA) dissolved in PBS was administered to mice at indicated postnatal days ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μM 5-ethynyl-2’-dexoyuridine (EdU, ThermoFisher Scientific) was added to the culture media for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: 5-ethynyl-2′-deoxyuridine (EdU) was purchased from ThermoFisher and stock solutions were created by dissolving the powder in dimethyl sulfoxide as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Genomics 2022Quote: EdU (5-ethynyl-2’-deoxyuridine) (Thermo Fisher Scientific, A10044) was added to culture media at 10 µM final concentration for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FCS and 2% B-27 (Gibco) and maintained at 5% CO2 and 37°C in humidified incubators ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl of 5 × Maxima RTA buffer (Thermo Scientific), which were mixed with Ultrapure water (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: EdU (5-ethynyl-2’-deoxyuridine; #A10044, Thermo Fisher Scientific) was diluted at 10mg/ml in 0.9% NaCl (#114-055-101 ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg plasmid and 5 μl Lipofectamine 2000 (Invitrogen) were diluted into 200 μl Opti-MEM (Gibco ...
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...