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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the cells were incubated at 4□ for 30 min with a PE-conjugated anti-Tra-1-60 antibody (Thermo Fisher Scientific, 12-8863-82, 1:5) or a PE-conjugated anti-SUSD2 antibody (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Cell Biology 2020Quote: ... Supernatant (1 ml at ~4-5 mg/ml) was incubated with 50 μl of superparamagnetic Dynabeads Streptavidin C1 (Invitrogen) and rotated overnight at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed at least 5 times 20 min and transferred to 0.25% PBT with 1:400 TO-PRO-3 (ThermoFisher #T3605) for 2 nights ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed 3 x 5 min with PBS before adding secondary antibody (1:200 Alexafluor-488 goat anti-mouse, Invitrogen) and rhodamine phalloidin (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The following day the sections were washed 3 times for 5 min each in PBS-T and incubated with the corresponding secondary antibodies (all 1:1000, Invitrogen), for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’) and 1 μl of 10 mM dNTP mix (Thermo Fisher). Illumina libraries were prepared by using a modified smart-seq2 protocol [Picelli 2014] using SuperScript IV RT and tagmentation procedure as previously described [Henning 2018] ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3 times with 5% milk TBST and incubated with HRP-conjugated secondary antibodies (1:5000, 32230/32260; Invitrogen). Data were visualized using chemiluminescence detection on ChemiDoc Touch (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Developmental Biology 2023Quote: ... Stained samples were washed (3 x 5 minutes) and incubated in DAPI solution (Invitrogen, D3571; diluted 1:1000 in DPBS) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Cell Biology 2023Quote: ... the iMACs were washed 3 x 5 min with PBS and incubated for 1h with 1:1000 goat anti-mouse IgG-AF488 (A11001, Invitrogen). Afterwards the cells were again washed 3 x 5 min with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Bioengineering 2024Quote: ... Primary cells were cultured in basal medium ((DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... COL1A1 (5’-CGAAGACATCCCACCAATC-3’ and 5’-ATCACGTCATCGCACAACA-3’), ALP (5’-TCACTCTCCGAGATGGTGGT-3’ and 5’-GTGCCCGTGGTCAATTCT-3’) (IDT, Coralville, USA) and viral non-structural (nsP1) gene (Applied Biosystems, USA) (5’-GGGCTATTCTCTAAACCGTTGGT-3’ and 5’-CTCCCGGCCTATTATCCCAAT-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated overnight with primary antibodies at 4°C in B/P buffer containing either 1:50 rabbit anti-fatty acid synthetase (MA5-14887; Invitrogen, Waltham, MA, USA) or 1:500 rabbit anti-peroxisome proliferator-activated receptor gamma (ab45036 ...
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bioRxiv - Physiology 2024Quote: ... were aspirated using a 21-gauge needle attached to a 5 mL disposable syringe in the presence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered TCM-199 medium (Gibco, Life Technologies, Milan, Italy) and 0.005% (w:v ...
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bioRxiv - Developmental Biology 2024Quote: ... The IVM medium consisted of 25 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid-buffered tissue culture medium 199 (M199; Gibco, Paisley, Scotland, UK) supplemented with 5% calf serum (CS ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
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bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Genomics 2024Quote: ... forward and reverse primers (800 nM; BLVpol_5f: 5′-CCTCAATTCCCTTTAAACTA-3′; BLVpol_3r: 5′-GTACCGGGAAGACTGGATTA-3′; Thermo Fisher Scientific), and 200 ng of gDNA template ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... staining was used to visualise collagen distribution and orientation while Alcian blue (1% Alcian blue in 3% acetic acid, Thermo Fisher scientific, MA, USA) staining was used to qualitatively examine sGAG content ...
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bioRxiv - Microbiology 2024Quote: ... 2% B27, 1% N2, 1% MEM non-essential amino acids, 1% penicillin/streptomycin and 0.1% β-mercaptoethanol, all from ThermoFisher Scientific) supplemented with dual SMAD inhibitors (10 µM SB-431542 and 100 nM LDN-193189) ...
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bioRxiv - Bioengineering 2020Quote: ... (anti-Claudin-1, anti-Claudin-5, anti-Occludin, anti-ZO-1; Invitrogen), in a 1:100 dilution in 10% donkey serum in PBS with 0.1% Saponin and incubated overnight on the Brain-Chip at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cludin-5 (1:1000) or -γ-tubulin (1:3000, Thermo Fisher Scientific) in Tris-buffered saline (Tris 50 mM and NaCl 150 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μL of T4 DNA ligase (5 U μL−1, ThermoFisher Scientific) and 2 μL of Milli-Q H2O (adjusted as needed to reach the total volume) ...
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bioRxiv - Bioengineering 2022Quote: ... 5 μg mL-1 fibronectin (bovine, ThermoFisher), and 100 μg mL-1 hyaluronic acid (ab143634 ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg mL-1 GlycoBlue (AM9516, Invitrogen) was added during the isopropanol (190764 ...
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bioRxiv - Neuroscience 2020Quote: ... tau (1:500, Invitrogen, clone TAU-5), tau (1:2,000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 g L−1 NaCl (Fisher Scientific)) with appropriate antibiotics for all the experiments ...
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bioRxiv - Genetics 2022Quote: ... 5% CO2 with 1% Penicillin/Streptomycin (Gibco). ST2 stem/stromal cell line was obtained from the American Type Culture Collection (Manassas ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 ng·ml−1 BDNF (#PHC7074, Gibco)] with Y-27632 (10 μM).
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bioRxiv - Neuroscience 2022Quote: ... and 5 ng·ml−1 BDNF (#PHC7074, Gibco)] ...
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bioRxiv - Immunology 2023Quote: ... 5 mM 1-Heptanol (Fisher Scientific, #AAA12793AE), 5 mM EDTA (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Claudin-5 (1:200, Invitrogen, 34-1600), and Zo-1 (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-Claudin-5 (Invitrogen, 1:100), rabbit anti-ZO-1 (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...