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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the standard phenol-chloroform-isoamyl alcohol protocol (PCI 25:24:1) with 0.5 μg ribonuclease A (PureLink RNase A, Life Technologies, Carlsbad, California) was performed to isolate DNA and eliminate RNAs ...
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bioRxiv - Immunology 2024Quote: ... Mononuclear cells were washed twice and resuspended in a minimum essential medium containing 10 µg/mL ribonucleases (MEM-α nucleosides; Gibco, #12571063) supplemented with 10% heat-inactivated FCS (HyClone ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... HM and MeT5A cells were pre-incubated with 10 μg mL−1 P-cadherin antibody (NCC-CAD-299) (Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... were used for mouse antibodies and protein G beads were used for rabbit antibodies (Dynabeads, Invitrogen, 10004D). Antibodies used were to CENP-A69 and CENP-C (gift from W ...
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bioRxiv - Biochemistry 2022Quote: ... and polyclonal antibodies were purified from bleeds using protein A purification (Custom Antibody Production, Life Technologies corporation). Western blot analysis was performed using NUPAGE 4-12% gels (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... Antibody conjugations were performed using Alex Fluor protein/antibody labeling kits from Invitrogen (A20173, S10900, and A20187).
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bioRxiv - Molecular Biology 2021Quote: ... The probe was biotin labeled at the 3’ end using a Pierce™ Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific Inc.). Seven micrograms of nuclear protein were added to a binding reaction mixture containing 2µl 10X binding buffer ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A biotin-pull down was then performed to select biotin-labelled DNA fragments using Dynabeads MyOne Streptavidin C1 beads (Thermo Fisher Scientific, 65001), resuspended in biotin binding buffer (5 mM TRIS-HCl pH 7.5 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific; A18869, Thermo Fisher Scientific) for monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed in wash buffer and then incubated with a 1:10,000 dilution of goat anti-biotin antibody (Thermo Fisher Scientific, Waltham, MA) in PBS pH 7.4 supplemented with 0.05% tween-20 ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... CSDE1 protein was then captured by incubation with an affinity purified CSDE1 antibody conjugated to protein A Dynabeads (Invitrogen) for 4 hours at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitation of the protein-antibody conjugate was performed incubating with Dynabeads® Protein A (Thermo Scientific, Carlsbad, CA, USA) 40 minutes at 4ºC in a rotary shaker ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA-protein-antibody complex was captured using protein A/G magnetic beads (Thermo Fisher Scientific, 10002D and 10004D). Magnetic beads were washed with low salt buffer (1X PBS ...
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bioRxiv - Plant Biology 2024Quote: ... The extracted protein was incubated with anti-ZmbZIP75 antibody and Pierce™ protein A Magnetic Beads (Thermo Scientific; #88802) at 4 °C for 4 h with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: The Q577R gp41 change was introduced into pSHIV-AD8-EO via site-directed mutagenesis using 5’p-TCAAGCAGCTCCGGGCAAGAGTCC-3’ (forward) and 5’p-TGCCCCAGACTGTGAGTTGCAACA (reverse) with Platinum SuperFi PCR mastermix (ThermoFisher) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... anti-p-BTK/ITK(Tyr551, Tyr511) (M4G3LN), anti-p-Akt (Ser473) (SDRNR) and anti-p-Erk (Thr202, Tyr204) (MILAN8R) were purchased from Thermofisher. Anti-mouse LDLR (101 ...
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bioRxiv - Biophysics 2020Quote: ... The α1 and β3 subunits were then transferred into the pcDNA4/TO and pcDNA5/TO vectors (Thermo Fisher Scientific), respectively ...
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bioRxiv - Biochemistry 2022Quote: Genes coding for MTREC subunits and their variants were cloned into pDONR221 vector using BP clonase recombination (Life Technologies). The inserts were then introduced into Y2H destination vectors (pDEST32 for bait and pDEST22 for prey ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Neuroscience 2021Quote: ... we injected 40nl of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (0.5%) or 647 (0.25%; Life Technologies) into the pulvinar ...
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bioRxiv - Neuroscience 2020Quote: ... A third monkey was injected with a cholera toxin subunit B-Alexa Fluor 647 conjugate (CTB-AF647; C34778, Invitrogen) at the quadrigeminal cistern ...
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bioRxiv - Neuroscience 2020Quote: ... and axial or intercostal muscles were injected with a solution of Cholera Toxin B Subunit-conjugated Alexa555 (Molecular Probes, Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... an optimized ratio of the baculoviruses for the different PHF1-PRC2 subunits was used to infect HiFive cells (Invitrogen). Cell were lysed using a glass Dounce homogenizer and the complex was purified using affinity chromatography (Ni-NTA and Strep-tag) ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Cell Biology 2020Quote: ... GM1+ lipid rafts were stained with the cholera toxin-subunit B-AlexaFluor 647 conjugate (1:5000; Molecular Probes, C34778) (58) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Neuroscience 2021Quote: ... Respective wild-type and mutant subunit cRNAs were synthesized in vitro with the mMessage mMachine T7 transcription kit (Invitrogen), titrated and checked for integrity ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Developmental Biology 2020Quote: ... An approximate total of 500-750 nL of Cholera toxin subunit B (CTB) AlexaFluor 488 or 647 Conjugate (Invitrogen) (Nanoject II ...
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bioRxiv - Biochemistry 2021Quote: ... The 30S-S1 subunits were pelleted via ultracentrifugation with a Sorvall S55-S swinging-bucket rotor ultracentrifuge (Thermo Scientific) at 55 000 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... mice were mounted in a custom spine stabilizer and viral particles or Cholera Toxin Subunit B conjugated to Alexa Fluor 647 (CTB-647) in sterile 0.9% NaCl (C22841-Thermofisher, Waltham ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and β2-OtNav1.8 subunits genetically linked to NH2-terminal eGFP using Lipofectamine 3000 reagent (L3000015, Invitrogen, Carlsbad, CA, USA) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... mice received a unilateral injection of cholera toxin beta subunit coupled to Alexa Fluor 594 (CTb-594, Invitrogen C34777). Mice were anesthetized with isoflurane and treated with proparacaine ophthalmic drops (1%) ...
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bioRxiv - Neuroscience 2023Quote: ... Retrograde tracers were cholera toxin subunit B (CTB-488; Alexa 488; Molecular probe, Invitrogen, 0.5% in HEPES buffered saline), WGA-Alexa488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 μL of cholera toxin subunit B (CTB) conjugated with Alexa Fluor 594 (2 mg/ml, Thermo Fisher Scientific) was injected into the right vitreous humors of the same mice (same procedure as viral injection ...
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bioRxiv - Molecular Biology 2024Quote: ... This allowed precise positioning of subunits into the pTB207 plasmid using an appropriate mix of FastDigest (ThermoFisher Scientific, USA) restriction enzymes ...
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bioRxiv - Neuroscience 2024Quote: ... The retrograde tracer cholera-toxin subunit B conjugated to Alexa fluor™ 647 (CTB-647, ThermoFisher scientific Cat # C34778) was bilaterally injected into the SNL (ML ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids encoding individual subunits were simultaneously co-transfected at an equimolar ratio into Freestyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... a solution containing cholera toxin subunit B (CTb; 1.0 mg/mL) conjugated to Alexa 488 or Alexa 555 (Invitrogen) as a bidirectional tracer was injected into the aDLS and pVLS (0.3 μL/site) ...
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bioRxiv - Neuroscience 2024Quote: HEK293T cell line stably expressing full-length human Cav1.2 e1b/8b/−9*/22/32/33 and auxiliary subunits human Cavα2δ1 and human Cavβ3 (Oertner et al. 2017) were cultured in DMEM (Gibco, 41966-029) supplemented with 10% fetal bovine serum (Thermo ...