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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...
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bioRxiv - Cell Biology 2021Quote: ... The amplification reactions for qualitative PCR were performed using Recombinant Taq DNA polymerase (Invitrogen, 11615-038) according to the following parameters ...
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bioRxiv - Immunology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/µl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Genomics 2022Quote: ... mTESR1 is exchanged with the addition of 5 ng/ml Recombinant Human BMP4 (Fisher Scientific, 314BP010). Exposure to BMP4 triggers differentiation of cells and is considered 0 hours for experimental purposes ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant single-strand AAV2/9 vectors were produced by transfection of HEK293T cells (Thermo Fisher Scientific) with the respective pAAV expression plasmid ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Immunology 2022Quote: ... The recombinant bacmid was extracted and further transfected into Sf9 cells using Cellfectin II (Invitrogen, #10362100). The recombinant viruses were harvested from the transfected supernatant and amplified to generate high-titer virus stock ...
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bioRxiv - Cell Biology 2022Quote: ... with <0.9% rabbit brain tissue contaminant) and another tube with human recombinant Thromboplastin (Thermo fisher scientific). Next to remove cells ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant BspC was biotinylated using a EZ-Link Sulfo-NHS-Biotin kit (ThermoFisher Scientific, Waltham, MA). The PVDF membrane was probed with the biotinylated BspC (100µg ...
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bioRxiv - Immunology 2020Quote: ... the reaction mix was supplemented with 40 U RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, ThermoFisher Scientific), 200 U SuperScript™ IV Reverse Transcriptase (18090050 ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD was diluted to 2.5 μg/mL in PBS (Thermo Fisher Scientific, Waltham, MA, USA) and 100 μl of the dilution was distributed in the wells of flat-bottom 96-well microplates (Immulon 2HB ...
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bioRxiv - Immunology 2020Quote: ... with oligo(dT) primers in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) or using SuperScript™ IV VILO™ Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs and MEFs were stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection or left unstimulated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™; Cat#10362100). After a three-day incubation period ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant bacmid was transfected into Sf9 cells using Cellfectin® II reagent (Thermo Fisher Scientific). The Sf9 cells were incubated for 120 hours at 28 to produce P1 baculoviruses ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: Recombinant PrPAP1Xa was secretively expressed using the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, USA) as previously described22 ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Biochemistry 2023Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). Spodoptera frugiperda Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinant GCase N370S mutant was produced by using 293T Freestyle cells (Thermo Fisher Cat. No. R79007) grown in suspension in 293 Freestyle medium (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Dicer/TRBP enzyme was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Cancer Biology 2021Quote: Proteins were quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... and protein was quantified using the Bradford protein assay (Thermo Scientific). For blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined using the Qubit Protein Assay Kit (ThermoFisher). Oligonucleotide probes (Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein content was assessed using BCA Protein Assay Kit (Thermo Fisher). Equivalents of 20 μg of protein were taken for mass spectrometry (MS ...
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bioRxiv - Genetics 2021Quote: ... Protein concentrations were estimated with bicinchoninic acid protein assay (ThermoFisher Scientific). Ten mgs of each lysate were combined ...
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bioRxiv - Genetics 2019Quote: ... DNA-protein complexes were recovered with protein G magnetic beads (Invitrogen). Library preparation was performed as previously described34 ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentrations were measured with Pierce 660nm Protein Assay (Thermo Scientific), and 10 μg of protein were loaded on 4–20% FastGene SDS-PAGE gradient gels (Nippon Genetics) ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A or protein G Dynabeads (Thermo Fisher Scientific, 10001D, 10003D) was used for rabbit or mouse antibodies respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by the BCA protein assay kit (ThermoFisher). For storage ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined by bicinchoninic (BCA) protein assay (ThermoFisher Scientific) and samples were resolved on 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were quantified using the BCA protein assay kit (ThermoFisher Scientific). Total protein extracts were resuspended in 5X Laemmli buffer at a final concentration of 500 µg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was assessed by Pierce BCA protein assay (Thermo scientific). For Western Blotting ...
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bioRxiv - Genomics 2020Quote: ... Dynabeads™ Protein A or Protein G (Invitrogen 10002D and 10003D) were washed 5 times with 5% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined by the BCA Protein Assay (Thermo Fisher). Whole cell lysates were resolved by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: ... Protein concentration was determined by BCA Protein Assay kit (ThermoFisher Scientific). Fifteen μ g of protein were resolved by electrophoresis on a 10% acrylamide gel and transferred to nitrocellulose membrane by standard methods ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentrationwas determined by MicroBCA Protein Assay kit (Thermo Scientific) according to the protocol provided by the manufacturer ...