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Citations for Thermo Fisher :
1151 - 1200 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (#10777019, Thermo Fisher Scientific). All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L ...
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bioRxiv - Microbiology 2020Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies, USA) as described above ...
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bioRxiv - Plant Biology 2020Quote: ... 1 unit/μl RNaseOUT ™ Recombinant Ribonuclease Inhibitor (Invitrogen), and 20mM Tris-HCl/10% glycerol for 25 minutes in ice ...
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bioRxiv - Genetics 2019Quote: ... and 200 µg/mL recombinant proteinase K (Thermo Scientific). The mixture was incubated at 37°C for 30 min and then 95°C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with recombinant Protein G agarose (Invitrogen) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... ISG15 recombinant rabbit monoclonal antibody (1□00) (Invitrogen, 703132), and collagen I (1□100 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific), 100 ng/ml recombinant human IGF-1 (BioLegend) ...
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bioRxiv - Biophysics 2020Quote: Production of recombinant baculovirus followed the manufacturer’s protocol (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: Recombinant protein expression and purification FreeStyle 293F (ThermoFisher Scientific) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). The lysates were cleared by centrifugation at 200,000 ×g for 10 min at 4°C 3 times to remove the contamination of the lipid ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). 10% of the precipitates were analyzed by western blotting to check the protein immunoprecipitation efficiency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Genomics 2022Quote: ... 0.5 μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019) was then added to each well ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant EcoSSB and Sequenase© were purchased from Thermofisher. T7DNAp was purchased from NEB ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant protein A/G conjugated to HRP (Thermo Fisher) diluted 1:500 in TBST was added each well and the plates were incubated for 1 hour at 37°C then washed four times with TBST ...
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bioRxiv - Cell Biology 2022Quote: ... 60 units of RNaseOUT Recombinant RNase Inhibitor (Invitrogen, 10777019) was added to each sample and vortexed for 5 seconds ...
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bioRxiv - Microbiology 2023Quote: ... recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), and then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
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bioRxiv - Cell Biology 2023Quote: ... 100U/mL RNAse OUT Recombinant Ribonuclear Inhibitor (ThermoFisher 10777019), and 1X complete EDTA-free Protease Inhibitor Cocktail (Sigma 11836170001)] ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl of Recombinant Protein G – Sepharose beads (Invitrogen) per condition were washed 3 times in IP buffer ...
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/ml recombinant EGF (PMG8041, Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant mouse (rm)IFN-γ was purchased from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2024Quote: ... recombinant murine IL-13 (Catalogue #210-13, ThermoFisher Scientific) and/or human Gastrokine 3 (GKN3 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL of RNaseOutTM Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies 10777019) suing a thermocycler ...
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bioRxiv - Bioengineering 2021Quote: ... platelet-derived growth factor receptor A (PDGFRA) and type I collagen (COL1A1) (Thermo Fisher, Waltham, Massachusetts). Probe references ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells expressing ACE2 receptors were suspended using TrypLE Select Enzyme solution (Thermo Fisher Scientific) and immediately added to all wells (10,000 cells in 100 μl of growth medium per well) ...
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bioRxiv - Neuroscience 2021Quote: ... surface receptors were labeled with Pierce™ Premium Grade Sulfo NHS-SS-Biotin (Thermofisher, Waltham, USA) and purified using Streptavidin High Performance Spintrap™ (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Receptor and chemokine baculovirus stocks were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... western blots were performed to normalize for receptor quantity using monoclonal anti-HA antibody (Thermo Scientific). Reactions were started by adding 150μL partially purified receptor samples to 300μL of reaction mixture and incubating on ice for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... and IGF-1 receptor (Catalog number: AM51331, siRNA ID:110754) with Lipofectamine RNAiMax Transfection Reagent (Invitrogen), according to manufacturer’s established protocol ...
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bioRxiv - Neuroscience 2023Quote: Stably expressing 5-HT receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... Membranes were probed with antibodies against: estrogen receptor β (Erβ, rabbit, 1:1000, Invitrogen PA1-311), tumor necrosis factor receptor 1 (TNFR1 ...
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then transiently transfected with receptor constructs using LipofectamineTM 2000 transfection system (ThermoFisher, cat# 11668019). Briefly ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: The chemicals used for the deorphanization of receptors were obtained from Acros Organics (Morris, NJ, USA), Alfa Aesar (Ward Hill ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies used were as follows: anti-mouse aryl-hydrocarbon receptor (ThermoFisher Scientific, MA1-513, 1:500) and anti-β-actin antibody (Millipore Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2019Quote: ... and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher). The libraries were sequenced on an Ion Proton system (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hi-Q reagents and PI™ chip kit V3 (ThermoFisher, Waltham, USA) and the sequencing was performed for 300 flows ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...
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bioRxiv - Cancer Biology 2020Quote: ... respectively supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco). Co-cultures of JeKo-1/REC-1 with MS-5 and HS-5 at a 10:1 ratio was maintained under the same conditions as for MS-5 or HS-5 cells alone ...
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bioRxiv - Plant Biology 2021Quote: ... using the Ion PI Hi-Q OT2 200 Kit (ionTorrent, Life Technologies).
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bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
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bioRxiv - Genomics 2020Quote: All splicing reporters were cloned into pAC-5.1-V5-His (ThermoFisher Scientific) using compatible restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... and 9.8 μL of highly deionized Hi-DiTM formamide (Applied Biosystems, France). Samples were analyzed by Eurofins genomics (Ebersberg ...