Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 7252 citations for Recombinant Human CSF1R His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
-
bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
-
bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
-
bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
-
bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
-
bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
-
bioRxiv - Cell Biology 2020Quote: ... The RNA transcripts were tagged with poly(A) tail using Poly(A) Tailing Kit (Invitrogen) to extend the stability of mRNA in zebrafish embryos.
-
bioRxiv - Cell Biology 2019Quote: ... Secondary antibody incubations were for 1 hour at RT using fluorescently tagged antibodies from Invitrogen. All antibody dilutions were made in a solution of 1 mg/ml BSA in 0.25% Tween 20 in PBS ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For each sample pool we retained only biotin-tagged fragments using Dynabeads M-270 (Invitrogen), and performed six separate ...
-
bioRxiv - Cancer Biology 2022Quote: Myc-tagged LCK containing plasmid was generated using pENTR/D-TOPO cloning kit (Thermo Scientific) according to manufacturer instructions ...
-
bioRxiv - Biochemistry 2021Quote: Biotinylated proteins were tagged using streptavidin-coated quantum dots (Qdot 655 and Qdot 605 - ThermoFisher) by incubating at 100 nM in HSABC (50 mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Affinity separated FLAG-tagged protein was eluted using 35 μL IgG elution buffer (Thermo Fisher), denatured with equal volume of Laemlli buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
-
bioRxiv - Cell Biology 2021Quote: Mouse hemagglutinin (HA) tagged Lphn2 construct was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) and cloned into the Gateway system pENTR221 vector (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2021Quote: ... HA-tagged NPH3 was immunoprecipitated using Pierce™ Anti-HA Magnetic Beads (Thermo Fisher Scientific). Thiophosphorylation was visualised by immunoblotting with anti-thiophosphoester monoclonal antibody (clone 51-8 ...
-
bioRxiv - Biochemistry 2022Quote: ... and transfected with pEGFP-C3 to express GFP-tagged S142A ExoU and RFP-Lamp1 (ThermoFisher), when 70% confluent ...
-
bioRxiv - Plant Biology 2020Quote: ... Then 200-fold MPBS/MTCS-diluted secondary antibody AlexaFluor488-tagged donkey anti-rat IgG (Thermofisher) antibody for JIM5 and JIM7 ...
-
bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... We immunoprecipitated GFP-tagged RBBP4 and RBBP7 proteins with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
-
bioRxiv - Molecular Biology 2021Quote: Cells with tagged RPAC1 were grown in suspension in Expi293 Expression Medium (Thermo Fisher Scientific) up to 7 x 106 cells/mL ...
-
bioRxiv - Biochemistry 2022Quote: ... TAP- or FTpA-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific) for 2.5 h at 20°C ...
-
bioRxiv - Cell Biology 2022Quote: L6 rat myoblasts expressing myc-tagged GLUT4 (33) were maintained in α-MEM (12561056, Gibco) supplemented with 10% fetal bovine serum and 1% pen/strep antibiotic in a humidified atmosphere containing 5% CO2 and 95% air at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... or ALS-associated Ruby-tagged sequences of DnaJC7 using 5 μL of Lipofectamine 2000 (Gibco) transfection reagent in OptiMEM (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... GFP-tagged cDNA constructs and siRNAs were transiently transfected using the Lipofectamine 3000 (Invitrogen, #L3000001) as per manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... MBP-tagged protein was purified by affinity column chromatography using Ni-IMAC resin (Thermo Fisher) followed by size exclusion chromatography using a Superdex 200 column (Sigma Aldrich) ...
-
bioRxiv - Biochemistry 2023Quote: Purified RRMs were fluorescently tagged using Alexa Fluor™ 633 C5 Maleimide (Thermo Fisher Scientific). The dye was added to a sample of purified protein (1:10 protein:dye molar ratio ...
-
bioRxiv - Molecular Biology 2023Quote: ... Biotin-tagged ends were pulled down using Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific 65001). Standard Illumina library preparation protocol including end repair ...
-
bioRxiv - Genomics 2023Quote: ... Verification of a successful GST-tagged AP2 protein purification by GST-affinity purification (Thermo Scientific Pierce Glutathione Superflow Agarose beads ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
-
bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from clonal CRISPR tagged cells using Genomic DNA Mini Kit (Invitrogen) according to manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2024Quote: ... to assess FLAG-tagged protein expression and immunoprecipitation by Immunoblot and silver stain (ThermoFisher Scientific) respectively ...
-
bioRxiv - Microbiology 2024Quote: ... Vectors of sulfatase 1 and 2 tagged with myc were transfected using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Biotin-tagged peptides were labeled with Pierce™ High Sensitivity NeutrAvidin™-HRP (Thermo Fisher) directly after blocking ...
-
bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
-
bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
-
bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
-
bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
-
bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...
-
bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
-
bioRxiv - Biochemistry 2021Quote: ... the isolated recombinant EMBacY was transfected into adhesive Sf9 cells (Invitrogen) in 6-well plates ...