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Citations for Thermo Fisher :
1151 - 1200 of 1930 citations for N Nitroso Di N Propylamine D14 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Matrix coating was then performed by incubating the wells at room temperature for 30 min with a mixture of 40µg/mL rhVitronectin-N (A14700, ThermoFisher) and 10µg/mL rhLaminin521 (A29249 ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was collected from EO771.LMB tumors that were xenografted into B6 and PWD.B6 mice (n = 4 per group) isolated using TRIzol Reagent (ThermoFisher), poly-A purified ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were immunostained by primary antibodies like neuronal nuclear protein (Neu-N) (Invitrogen, California, USA, 1:500 dilution) (Gusel’Nikova et al. ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... N-2 media was replaced by B-27 media consisting of B-27 Serum-Free Supplement (Thermo Fisher Scientific), GlutaMAX (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... 10x serial dilutions were made in 0.1% formic acid 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) in LCMS grade water ...
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bioRxiv - Cell Biology 2023Quote: ... and the single strand cDNA was cleaned up with DynaBeads® MyOneTM Silane Beads (Life Technologies P/N 37002D). cDNA was amplified using a Bio-Rad PTC-200 Thermal cycler with 0.2 ml 8-strip nonFlex PCR tubes ...
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bioRxiv - Neuroscience 2023Quote: Whole mouse brains were homogenized with a Dounce tissue grinder in neuronal protein extraction reagent (N-PER) (Thermo Scientific) containing protease/phosphatase inhibitors (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... Amount of N-Glycan was measured by integrating the peaks with Thermo Xcalibur software (Thermo Fisher Scientific, Waltham, MA) giving the normalized ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were incubated O/N with an Alexa Fluor 488 anti-mouse IgG secondary antibody (Life Technologies, A28175) diluted at a final concentration of 1/200 respectively in 1% blocking solution ...
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bioRxiv - Evolutionary Biology 2023Quote: We individually subcloned HRR25 orthologs to pKB11 (pDEST-DHFR F[1,2] C-term NatR) and pDN0605 (pDEST-DHFR F[1,2] N-term NatR) by LR cloning (Invitrogen, 11791), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA5/FRT/TO N-YFP vector was double digested by restrictive enzymes of KpnI and NotI (Thermo Fisher Scientific). Wild type Cdc20 was amplified by PCR and inserted into the expression vector according to standard procedures ...
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bioRxiv - Biochemistry 2024Quote: ... or SK-N-BE(2) cells were transfected with the siRNAs using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778030) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA of all ancestral (A) and evolved (N) lines were isolated using Yeast DNA Extraction kit (Thermo Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and G3BP1 were labeled on their N-termini with AF488 (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Storage buffer was exchanged to Labeling buffer (50 mM MES pH 6.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF; ACROS Organics, Cas#329-98-6), 5 mM β-ME ...
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bioRxiv - Bioengineering 2020Quote: ... fusidic acid (FA; 98%, Acros Organics or F0756-#SLBN8134V, Sigma-Aldrich) was incorporated in the organic phase as previously described(52) ...
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bioRxiv - Systems Biology 2021Quote: ... cell concentration and viability (98 %) was determined using Countess II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 23% (w/w) fusidic acid (FA; 98%, 5552333, Thermo Scientific Acros) in PLA was incorporated (FA/PLA total content of 10% w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 100 μg mL−1 hygromycin (31282-04-9, ≥98%, Invitrogen). Cells were split 1:5 when confluency reached 70% and discarded after passage 10 ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...
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bioRxiv - Bioengineering 2020Quote: ... in deionized (DI) water containing 1% agarose (Thermo Scientific, molecular biology grade) (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG, Life Technologies) for 60 min at 37°C in NaHCO3 pH 9.6 buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The voltage-sensitive dye Di-4-ANEPPS (Cat# D1199; Thermo Fisher Scientific) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were stained with Di-4-ANEPPDHQ (Thermo Fisher Scientific; #D36802) at 1:1000 dilution in 1ml PBS ...
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bioRxiv - Biophysics 2023Quote: ... and Di-4-ANEPPDHQ (Di4) (catalog # D36802) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Cancer Biology 2021Quote: The Asparagine to Glutamine (N-Q) point mutation was performed using a Phusion SDM kit (Thermo Fisher Scientific, CAT# F541). In this assay ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Developmental Biology 2021Quote: The Emerald Green Fluorescent Protein (EmGFP) -encoding region from Vivid ColorsTM cDNA pcDNATM6.2/N-EmGFP-DEST Vector (Thermo Fisher Scientific) was placed upstream of the coding sequence of PmlVI (human PML transcript variant 5 ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Bioengineering 2020Quote: ... Tipless MLCT-D probes (0.03 N/m spring constant) were functionalized with 10 μm diameter borosilicate glass beads (Thermo Scientific 9010 ...
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bioRxiv - Developmental Biology 2021Quote: Lymph node divested mammary glands (excluding the cervical pair) were dissected from the mice and digested O/N in DMEM/F12 (Gibco) + 10 mM HEPES (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from the 60 hpa tissues (n=6) from each group using TRIzol reagent (Applied Biosystems, USA). One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Physiology 2022Quote: ... at 1-1.5 x 106 cells per well in William’s medium (catalog n°12551032, Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 5% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.3 mM ATP were reacted with a 10-fold molar excess of N-(1-pyrene)iodoacetamide (Thermo Fisher Scientific) dissolved in dimethylformamide (DMF ...
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from Hela cells (n = 3) subjected to EBSS-induced autophagy and treatment with 5 μM UNC0638 for 12 hours using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... a puromycin N-acetyltransferase and the WPRE next to stuffer sequences was ordered as a GeneArt Synthetic Gene (Life Technologies) and PCR-amplified ...
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Molecular Biology 2019Quote: ... The sequence of RVFV IGR and flanking nucleotides of the N and NSs genes were synthesized by GeneArt (Thermo Fisher) for stepwise assembly of the complete minigenome in the pX12ΔT vector ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...