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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco™, 25300062). Cell density was 5x 104 per well for the experiments in the μ-Slide (ibidi ...
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bioRxiv - Cell Biology 2023Quote: ... MCEC were split every 2-3 days using TrypLE express enzyme (GibcoTM, Thermo Scientific) for 5min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... use 10 μL for 2-3 ml of blood) or EDTA (0.5M EDTA, Gibco, cat ...
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bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
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bioRxiv - Bioengineering 2024Quote: ... with media replenished every 2-3 days or passaged with TrypLE (Gibco catalog #1260421) at 60-80% confluence ...
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bioRxiv - Bioengineering 2024Quote: ... with media replenished every 2-3 days or passaged with TrypLE (Gibco catalog #1260421).
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bioRxiv - Molecular Biology 2024Quote: ... a 3:2 ratio of 0.5% (w/v) ORO in isopropanol (Fisher Scientific, USA): double distilled H2O (ddH2O ...
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bioRxiv - Microbiology 2024Quote: These cell lines were subcultured every 2–3 days using 0.05% Trypsin/EDTA (Gibco). All cell lines were maintained in a humidified 37°C incubator supplied with 5% CO2.
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bioRxiv - Bioengineering 2024Quote: ... and CellEvent™ caspase-3/7 green detection reagent (2 µM Thermo Fisher Scientific) for 1 h and then subjected to confocal microscopy imaging (Leica TCS SP8 ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were removed by 3 washes with 2% BSA 1x PBS and incubated with 2 μg/ml anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) for 2 h in the dark at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Physiology 2023Quote: ... The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies) and injected into FLP-7 secretion line (SSR1164 ...
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bioRxiv - Immunology 2024Quote: ... and subsequently exocrine-free islets were selected and dispersed with 0.02 % trypsin for 3 min at 37 °C and washed with FACS buffer (2% FBS, 2 mM EDTA (Thermo Fisher Scientific) in PBS (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell pellets were resuspended with 50 μL of SC−Ura medium without carbon source (7.4 g/L Yeast nitrogen base without amino acids, #291940, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were picked and streaked on YPAD agar plate and SCDex−Ura agar plate (20 g/L glucose, #16806-25, Nacalai tesque; 6.7 g/L Yeast nitrogen base without amino acids, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and spread on a SCGal−Ura agar plate (20 g/L galactose, #075-00035, FUJIFILM Wako Chemicals; 6.7 g/L Yeast nitrogen base without amino acids, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass, 3 µg total plasmid) using Lipofectamine 3000 (Thermo Scientific cat. no. L3000015). Media was exchanged after 6 hours and viral supernatant was harvested 48 hours after transfection and filtered through 0.45 μm cellulose-acetate filters (VWR cat ...
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bioRxiv - Cancer Biology 2020Quote: 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein Diacetate (DAF) (ThermoFisher) was used to measure and spatially resolve nitric oxide (NO ...
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bioRxiv - Bioengineering 2023Quote: ... was reduced on the 78 ± 3 nm cores using 1 ml of 1 mM L-ascorbic acid (AA) (Fisher Scientific), making core@shell structures ...
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bioRxiv - Pathology 2020Quote: ... citric acid (Fisher Scientific, Cat. No. A104-3, Hampton, NH), sodium phosphate (Sigma Aldrich ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... and 2 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen). Perm buffer (CSM-S ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% B27 without retinoic acid (Life Technologies), 1% Glutamax (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 without retinoic acid (Gibco), 20ng/ml EGF (Prospec Bio) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-alanyl-L-glutamine (Thermo Fisher Scientific 35050079), 1× MEM nonessential amino acids (Thermo Fisher Scientific 11140076) ...
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bioRxiv - Immunology 2023Quote: ... 1x GlutaMAX (2 mM L-alanyl-L-glutamine dipeptide; Gibco), 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... 6mM L-glutamine and 0.1mM Non-Essential Amino Acids (NEAA) (all reagents from Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... but additionally had 4 mM L-glutamine and MEM non-essential amino acids (Thermo Fisher, 11140050).
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bioRxiv - Developmental Biology 2021Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as described (Chen et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as previous described (Chen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... and β-actin (Mouse Monoclonal anti-β-actin antibody Ambion, Thermo Fisher Scientific; Waltham, MA, USA).
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bioRxiv - Systems Biology 2020Quote: ... isopropyl β-D-thiogalactopyranoside (IPTG) and ortho-nitrophenyl-β-galactoside (ONPG) were obtained from Fisher Scientific; methyl-β-D-thiogalactoside (TMG ...
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bioRxiv - Molecular Biology 2024Quote: ... β-galactosidase activity was measured with the semi-quantitative yeast β-galactosidase activity kit (Thermo Scientific) on individual co-transformant colonies ...
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bioRxiv - Cancer Biology 2020Quote: Chromatographic separation was performed using an Intrada Amino Acid column (150mm * 1mm, 3 μm) (Imtact Corp., Japan) on an Ultimate 3000 LC system (Thermo Fisher Scientific, Bremen, Germany). 5 μl of amino acid extract was injected and separated by binary gradient elution ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... Three hours after plating medium was exchanged for maintenance medium (Neurobasal-A without Phenol Red, 2% B-27 supplement, 1% penicillin-streptomycin, 0.5 mM glutaMAX, 25 μM β-mercaptoethanol; ThermoFisher Scientific) and cells were kept at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were grown in 6-well plates to 50% confluency and co-transfected with Myc-BMPR2 or Myc-BMPR2-S532X and HA-β-arrestin1/2 using Lipofectamine 2000 (Invitrogen). After 48 h of transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Biochemistry 2020Quote: Whole-cell lysates were obtained incubating cell pellets with a Lysis Buffer (2% n-dodecyl β-dmaltoside (DDM) (Thermo Scientific, #89903) plus 1X protease inhibitor cocktail (Roche ...