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Citations for Thermo Fisher :
1151 - 1200 of 7935 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: HEK293 FT cells were transfected with dVPR and VSVG packaging plasmids using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-APEX2-NIK3 expressing U2OS (U2OS:GFP-APEX2-NIK3) and HEK293 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Flp-In™ T-REx™ 293 cells (referred to as HEK293 through the manuscript; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 μl of 4 × 104 cells/ml HEK293 Flp-In T-REx cells (Thermo Fisher Scientific, R78007) or HEK293 Flp-In T-REx SBP-eIF4A1 (Phe163Leu-Ile199Met ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5ug of each plasmid was transfected independently into HEK293 cells using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (NT and FASTKD5 sgRNAs) samples were purified using the Dynabeads mRNA purification kit (ThermoFisher, 61006) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... HeLa-FRT UBAP2L KO and HEK293 cell lines were cultured in DMEM GlutaMax media (Thermo Fisher Scientific) at 37C in a humidified incubator with 5%CO2 ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 cells were transfected with 3 μg of linearised plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000001) in wells of a 6-well plate ...
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bioRxiv - Systems Biology 2023Quote: ... We cultured the cells in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen; RAW 264.7, HEK293, and NIH-3T3) or F-12K medium (Caisson ...
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bioRxiv - Molecular Biology 2024Quote: ... TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific). HEK293 cell lines with tetracycline inducible TAP-tagged baits were generated by co-transfection of 0.1 µg of plasmid containing the TAP-tagged bait (hsINTS13ΔC ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were seeded in 10 cm2 dish with Dulbecco’s Modified Eagle Medium (#11965118, Thermo Fisher Scientific) supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader ...
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bioRxiv - Immunology 2019Quote: To measure the levels of secreted IL-17a from the Th17 cell culture assay we utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Neuroscience 2020Quote: ... The conditioned media was used to evaluate levels of IL-1β using the mouse IL-1β ELISA kit (Thermo Fisher Scientific Cat#88-7013-22 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: IL-1β levels were determined from prefrontal cortex extracts by using the IL-1β ELISA kit (ThermoFisher Scientific, #88-7013) following the manufacturer’s protocols.
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bioRxiv - Immunology 2021Quote: ... the cell culture supernatant was harvested to examine IL-27 secretion using the Mouse IL-27 Uncoated ELISA kit (Invitrogen).
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Immunology 2022Quote: ... and stimulated for 24 hours with IL-17A (200ng/mL, cat: 200-59-100ug, Shenandoah) and anti-IL-17RC (5ug/mL, cat: PA5-47286, Invitrogen). Goat IgG (5ug/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL) was then used to evaluate RNA purity ...
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Immunology 2022Quote: ... and IL-1β Mouse ELISA Kits (ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (eBio17B7, Thermo Fisher Scientific, Waltham, MA), and IFNγ (XMG1.2 ...
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bioRxiv - Immunology 2021Quote: ... and IL-1β mouse ELISA kit (ThermoFisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and protease inhibitor cocktail (Thermo Scientific, Rockford, IL)] ...
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bioRxiv - Immunology 2021Quote: ... and IL-33 (Invitrogen; sensitivity 25 pg/mL) ELISA kits according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... PE conjugated anti-mouse IL-2 (Invitrogen eBioscience), and BV421 anti-mouse TNF-α (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse IL-12 (clone C17.8, Invitrogen) antibodies.
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bioRxiv - Immunology 2020Quote: ... IL-1α (Thermo Fisher Scientific, clone ALF-161), or IL-1β (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or IL-1β (Thermo Fisher Scientific, clone NJTEN3) in perm/wash buffer for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... PE conjugated anti-mouse IL-2 (Invitrogen eBioscience), and BV421 anti-mouse TNF-α (BD Biosciences ...
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bioRxiv - Systems Biology 2022Quote: ... ELISA assays for IL-6 (Invitrogen, catalog # KHC0061) and IL-8 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and IL-17 (Invitrogen Cat. 88-7371-88) was quantified by a sandwich ELISA according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... or IL-17 at (2.5 ng/ml; Invitrogen), following the protocol previously published for RASFs113 ...
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bioRxiv - Immunology 2020Quote: ... 10 ng/ml IL-2 (Gibco, Gaithersburg, MD), and 25 ng/ml IL-10 (STEMCELL Technologies ...
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bioRxiv - Physiology 2021Quote: ... and trypsin (Thermo Fisher Scientific, Rockford, IL, USA), overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... respectively (Thermo Fisher Scientific, Meridian Road Rockford, IL) and analyzed as detailed in Calle et al ...
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bioRxiv - Microbiology 2020Quote: ... and anti-IL-17A antibody (Thermo Fisher Scientific) were used ...
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bioRxiv - Immunology 2020Quote: ... PE conjugated anti-mouse IL-2 (Invitrogen eBioscience), and BV421 rat anti-mouse TNF-α BD Biosciences in a total of 100 μL of 1× Perm/Wash Buffer (BD Biosciences) ...
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ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signalingbioRxiv - Microbiology 2021Quote: ... or IL-6 (ThermoFisher Scientific, # 88-7066-22) using manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml IL-4 (Life Technologies), and evaluated on day 6 of culture ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Immunology 2022Quote: ... PerCP-eFluor710-IL-13 (eBio13A, Thermo Fisher Scientific), PE-IL-5 (TRFK5 ...