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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 6 Chloro N 5 ethoxy 1H pyrazol 3 yl 3 nitropyridin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was carefully removed and resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 20 min at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400, Thermo Fisher, USA) with stem-loop primers and sly actin_R primers as internal controls (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days by detachment with 0.05% trypsin-EDTA (ThermoFisher Scientific).
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10−3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Developmental Biology 2024Quote: ... Passaging was performed every 2 to 3 days using Accutase (00-4555-56, ThermoFisher Scientific). The cells were Mycoplasma tested prior to running experiments ...
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 % penicillin-streptomycin) supplemented with 2% N-2 supplement (Gibco), 1% B-27 supplement serum free (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... 6′-diamidino-2-phenylindole (Invitrogen). All observations were performed on a Nikon E600 epifluorescence microscope ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... brains were washed trice with PBS for 5 min and free amines were anchored with 0.1mg/mL succinimidyl ester of 6-((Acryloyl)amino)hexanoic acid (Acryloyl-X, SE, Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Biophysics 2024Quote: ... and N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) supplemented with 10% fetal bovine serum (FBS) (26140079, Gibco), 1% penicillin/streptomycin (15140122 ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The caspase-3 activity was quantified using EnzChek™ Caspase-3 Assay Kit #1 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Biophysics 2024Quote: ... and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Thermo Scientific, 24510) in Borate buffer (100 mM Borate-NaOH ...
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bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-sense, 5’ AAGAGGUUUAGCUGGUAC CTT 3’) or control nontargeting siRNA (Genepharma, Shanghai) using Lipofectamine RNAiMAX (Invitrogen). Six hours after transfection ...
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bioRxiv - Bioengineering 2019Quote: Cells were loaded with the Ca2+sensitive fluorescence indicator Fluo-3/AM (5 µM, Life Technologies) together with 0.1% Pluronic f-127 (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Samples were injected onto a Biobasic AX LC column (5 μm, 50 × 3 mm; Thermo Scientific). The mobile phase consisted of 100 mM ammonium carbonate (A ...
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bioRxiv - Genomics 2020Quote: ... and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen). The fragmented mRNAs were reversely transcribed into cDNA by SMARTScribe reverse transcriptase (TaKaRa) ...
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bioRxiv - Cancer Biology 2020Quote: The 3’ & 5’RACE products were cloned using TOPO TA Cloning Kit (Invitrogen, Cat #K4530-20), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... passage 3 fibroblasts were trypsinized 5 min at 37°C using 0.05% trypsin with EDTA (Gibco), centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... DM-3 and RS-5 cells were cultured in NCTC-109 medium (Gibco, Carlsbad, CA, USA) supplemented with antibiotics ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Genomics 2020Quote: ... commercially available CRISPR control targeting HPRT (IDT) and KANK1 exon-targeting (ThermoFisher Scientific, 5’-GUCUAGUUGAUAACCAUAGG-3’) gRNAs were also obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 x 5 minutes in PBST and then incubated with secondary antibodies (Invitrogen) diluted in PBST + 0.2% BSA for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA (5’-GGACTGAGGUUGUCAAGAA-3’) for PRC1 using Oligofectamine (Life Technologies, Carlsbad, CA) as previously described (Udy et al. ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...