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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 500 μM triazole ligand Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amin (TBTA, Thermo Fisher Scientific, 454531000), 62.5 μM biotin-alkyne tag (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Physiology 2023Quote: ... The nucleic acid stain TO-PRO™-3 Iodide (642/661) (200 nM, T3605, Thermo Fisher Scientific) was added together with the final secondary antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ToPro-3 1:1000 and TMRM 1:100,000 (Invitrogen) added and incubated for 10 min ...
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bioRxiv - Physiology 2023Quote: ... Cells were then spun at 350 x g for 4 min and resuspended in 1 mL FSW with 10 µM pH-sensitive cell dye SNARF-1 acetoxymethyl ester acetate (Invitrogen, Thermo Fisher Scientific) and 0.1% dimethylsulfoxide (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Acetoxymethyl (AM) ester form of the calcium-sensitive dye Oregon Green BAPTA-1 (OGB-1 AM, Molecular Probes, Eugene, OR, USA; 50 micrograms) was dissolved in 4 µl of dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2024Quote: ... dsRNA complex (2:1 molar ratio) were cross-linked with 30 μg 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific, EDC: protein = 3:1 w/w) in the presence of 66 μg N-hydroxysulphosuccinimide (NHS ...
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bioRxiv - Biochemistry 2020Quote: ... Freshly isolated cells were incubated at 1 × 107 cells/ ml in PBS with 0.5% dialyzed FBS supplemented with 5μM carboxyfluorescein succinimidyl ester (CFSE, Molecular Probes) or 2.5μM CellTrace Far Red (Molecular Probes ...
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bioRxiv - Cancer Biology 2022Quote: Proliferation was measured by labelling primary CD8+ T-cells with 1 μM carboxyfluorescein diacetate succinimidyl ester (CFSE, Thermo Fisher) for 10 min at 37°C before activation and treatments ...
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bioRxiv - Neuroscience 2024Quote: ... a stock solution (1 mM in DMSO) of membrane-permeant acetoxymethyl ester Fura-2 AM (Life Technologies, Eugene OR) was prepared and diluted to 8 µM in 1 ml of fresh growth medium (see Primary hippocampal cultures) ...
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bioRxiv - Neuroscience 2022Quote: ... We then proceeded with a standard hot phenol-acid extraction (Acid Phenol:Chloroform mix 125:24:1, Invitrogen, AM9722). The samples were resuspended in nuclease-free water and quantified on a Spectrophotometer ...
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bioRxiv - Biophysics 2021Quote: ... Methyl-PEG4-thiol (MT(PEG)4) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... and Methyl-PEG-Maleimide Reagent (MM(PEG)24) (Thermo Fisher, 22713). NEM was added to Chlamydomonas cultures growing as indicated in each case to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... methyl tert-butyl ether and 2-propanol were from Thermo Fisher Scientific (Pittsburg ...
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bioRxiv - Developmental Biology 2022Quote: ... 80□μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide□(Thermo Fisher Scientific) and 70□μL ethyl acetate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and Paraquat (#227320050: Methyl viologen hydrate 98%) was from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM of 4-(2-hydroxyethyl)-1-piperazine-1-ethanesulfonic acid (HEPES) (Gibco) and 1 ng/mL of human bFGF (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... 1% Penicillin/Streptomycin and 1% non-essential amino acids (all from ThermoFisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... 1× MEM non-essential amino acids and 1× penicillin-streptomycin antibiotics (ThermoFisher Scientific). Terminal cellular differentiation was induced with 10 μM N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT ...
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bioRxiv - Immunology 2020Quote: ... 1% non-essential amino acids and 1% HEPES (all from Thermo Fisher Scientific). NK-92 cells were cultured according to the recommendations from ATCC ...
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bioRxiv - Immunology 2020Quote: ... 1% non-essential amino acids (NEAA) and 1% penicillin-streptomycin (P/S; Gibco). HEK 293T-ACE2 cells were produced as previous described17 (Figure 3 ...
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bioRxiv - Pathology 2021Quote: ... 1% non-essential amino acids and 1% Penicillin/Streptomycin (all from Life Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... 1 mM nonessential amino acids and 1 mM sodium pyruvate (all ThermoFisher Scientific). After 72 hrs in culture ...
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bioRxiv - Cell Biology 2022Quote: ... 1% (v/v) penicillin/streptomycin and 1% non-essential amino acids (NEAA, Gibco). All cell cultures were kept in a 37ºC incubator with 5% CO2 and passaged every 2-3 days ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1% non-essential amino acids and 1% sodium pyruvate (Gibco, Invitrogen, Merelbeke, Belgium). The cells were kept in a humidified 37°C incubator with a 5% CO2 environment ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1% non-essential amino acids and 1% sodium pyruvate (Gibco, Invitrogen, Merelbeke, Belgium). The cells were kept in a humidified 37°C incubator with a 5% CO2 environment ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) (Invitrogen, cat.#15630080), CellMask membrane dye (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Microbiology 2021Quote: ... the drugs were diluted from 0.78-25µM in 200µL of MOPS [3-(N-morpholino) propanesulfonic acid] buffered RPMI 1640 (Life Technologies), pH 7 ...
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bioRxiv - Neuroscience 2021Quote: ... a 400 mM stock solution of Auxin indole-3- acetic acid (IAA) (Thermo Fisher, Alfa Aesar™ #A1055614) was created by dissolving Auxin in ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl-β-D-thiogalactopyranoside (IPTG) and 3-(N-morpholino) propane sulfonic acid (MOPS) were purchased from Fisher Scientific. MTSES was purchased from Biotium.
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bioRxiv - Immunology 2024Quote: 50 000 enriched CD11b+ LCs were seeded and incubated for 10min at 37°C with 2mM Bodipy-C11 (581/591) (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid; Invitrogen) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Spn grown to an OD620 of 1.0 were resuspended in 1 ml PBS containing 1% catalase and 10 µM carboxyfluorescein diacetate succinimidyl ester dye (CFSE, Thermo Fisher Scientific, Waltham, MA) and incubated at 37°C for 25 mins ...
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bioRxiv - Cell Biology 2020Quote: Proteins were labeled with succinimidyl ester reactive fluorophores from Molecular Probes (Alexa Fluor™ 647 or DyLight™ 488 NHS Ester ...
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bioRxiv - Neuroscience 2021Quote: ... 2.3 nl of Alexa Fluor 405 NHS Ester (Thermo Fisher: A30000) or Alexa Fluor 647 10 kDa Dextran (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... A solution of Alexa Fluor 488 NHS Ester (Invitrogen, Carlsbad, CA) in DMSO (32.5 μL ...
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bioRxiv - Bioengineering 2020Quote: ... freshly extracted chondrocytes were stained with carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen) prior to hydrogel encapsulation ...
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bioRxiv - Biochemistry 2020Quote: ... Carboxyfluorescein succinimidyl ester (CFSE) was purchased from Invitrogen (Carlsbad, CA, US). Glyoxal (MEF studies) ...
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bioRxiv - Microbiology 2020Quote: ... were conjugated to AlexaFluor −594 or −488 succinimidyl esters (Molecular Probes) following the manufacturer’s instructions and used at a 1:250 and 1:125 dilution ...
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bioRxiv - Microbiology 2020Quote: ... S-layer17 Cells were biotinylated with biotin-NHS-ester (21330, ThermoFisher) and incubated for 15 min at R.T ...
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bioRxiv - Molecular Biology 2022Quote: An N-hydroxysuccinimidyl (NHS) ester of Alexa488 fluorochrome (NHS-Alexa488, ThermoFisher) was used to fluorescently label purified Pae87 ...