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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco) and seeded 20–24 h before transfection at 1.8 × 107 cells per T225 flask ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2 mM glutamine and 1% penicillin/streptomycin (Life Technologies, Thermo Fisher Scientific). HUVECs were isolated from donors using previously described methods (59) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2 mM glutamine and 1% penicillin/streptomycin (Life Technologies, Thermo Fisher Scientific). HUVECs were isolated from donors using previously described methods (59) ...
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bioRxiv - Cell Biology 2023Quote: ... sections were incubated in 2 mM POPO-1 nuclear counterstain (Life Technologies) diluted in PBS containing 0.05% saponin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... or with three custom targeting sgRNAs (Invitrogen, Supplementary Tables 1 and 2).
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bioRxiv - Developmental Biology 2023Quote: ... with DAPI (4’, 6-Diamidino-2-Phenylindole) (Life Technologies; D1306; 1:10,000). Images were captured using a Nikon Eclipse 80i system with the NIS-Elements BR software (version 4.3 ...
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bioRxiv - Cell Biology 2023Quote: ... (1) E8 medium supplemented with 2% E8 supplement (50x) (Thermo Fisher Scientific), 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Microbiology 2023Quote: ... Diamidino-2-phenylindole (DAPI) (Thermo Scientific, #62247, 1:1000 dilution for IFA); Mounting medium (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 1-2 µg of RNA was treated with DNAse I (ThermoFisher Scientific), and the cDNA was prepared using the RevertAid First Strand cDNA Synthesis kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... containing 1% BSA and 2 mM EDTA (Thermo Fisher Scientific, Waltham, MA) was added.
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were Alexa Fluor 594 (1:750, 2 hrs; Invitrogen A11008) and Alexa Fluor 488 (1:750 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). U2OS cells were a gift from the Chang lab and grown in McCoy’s 5A (modified ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). Stem cell line H1 were purchased from WiCell (Cat # WA01) ...
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bioRxiv - Neuroscience 2023Quote: ... 2) rabbit anti-pT217-tau at 1:200 (cat # 44-744, ThermoFisher). The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains threonine 217 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 % penicillin-streptomycin and 100 µM 2-mercaptoethanol (all from Thermo Fisher), and further supplemented with 100 ng/mL Noggin (Bio-techne) ...
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bioRxiv - Biophysics 2023Quote: ... the nucleus with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, 1:200), microtubules with an anti-tubulin antibody produced in mouse (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine and 1 mM sodium pyruvate (Thermo Fisher, #11360039), 100 µg/ml of Normocin (InvivoGen ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 2 ug/µL BSA (Cat No. B14, ThermoFisher Scientific), and 1 µL of 10 ng/µL DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected with 2 µg mL-1 puromycin (Thermo Fisher Scientific) for 3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary monoclonal transglutaminase-2 antibody (CUB7402, Thermo Fisher #MA5-12736, 1/15000) was diluted in 0.5 (m/v ...
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bioRxiv - Microbiology 2024Quote: ... mouse monoclonal anti-BrdU (Thermo Fisher, B35128, 1:50, 2 µg/mL). Secondary antibodies and the optimal dilutions and concentrations used are as follows ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM l-glutamine and 1% (wt/vol) nonessential amino acids (GIBCO). Medium was replaced with fresh medium every three days.
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bioRxiv - Cell Biology 2024Quote: After washing 2 times with ice-cold 1× PBS (14080-055, Gibco), cells were trypsinized or scrapped with a rubber policeman ...
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bioRxiv - Neuroscience 2024Quote: ... 1% (v/v) N-2 supplement (Gibco, Cat no: 17-502-048), and 40 ng/mL basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... and AID (Clone mAID-2, ThermoFisher, 1:800, Cat # 14-5959-82)/Opal 690 ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-SARS-CoV-2 M protein 1:250 (ThermoFisher: PA1-41160), rabbit anti-SARS-CoV-2 E protein 1:250 (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-SARS-CoV-2 E protein 1:250 (ThermoFisher: PA1-41158), rabbit anti-β-actin 1:250 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µL of rhodamine-phalloidin 400X (diluted 1:500) (Invitrogen, R415) to label EPEC and F-actin ...
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bioRxiv - Immunology 2024Quote: ... and cDNA synthesis from 1-2 μg RNA using SuperScript IV (Invitrogen) using Oligo(dT ...
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bioRxiv - Immunology 2024Quote: ... THP-1 (ATCC) were maintained in R10 with 2-mercaptoethanol (Gibco, 31350010). MRC-5 (ATCC ...
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bioRxiv - Biophysics 2024Quote: ... the nucleus with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, 1:200), microtubules with an anti-tubulin antibody produced in mouse (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific), 1 × Glutamax ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-beta-2-microglobulin antibody (1:100, MA5-36022, Thermo-Fisher Scientific), anti-HLA-I-ABC antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... 2° Dk – α-Rb-IgG Dylight 550 (Invitrogen, SA5-10039, 1:300). Active-caspase area was quantified by manually thresholding for positive-fluorescence.
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bioRxiv - Bioengineering 2024Quote: ... 6-diamidino-2 phenylindol (DAPI, 1 ug/mL; Thermo Fisher, Waltham, MA), and AlexaFluor 488 phalloidin (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with KIF-encoding plasmid and empty vector pcDNA3.1 (+) (1 µg total DNA) using 2 µl Lipofectamine 3000 Reagent and 2 µl P3000 (Thermo Fisher Scientific). hTERT-RPE1 cells stably expressing SMO-tRFP were transfected a day after seeding and IMCD3 cells expressing mCherry-Arl13b were reverse transfected at the time of seeding ...
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bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Cancer Biology 2023Quote: ... was pre-complexed at 5µg/mL with PE-conjugated goat anti-Human IgG diluted at 1/100e (Supplementary Table 2) for 2 h at room temperature in PBS CaCl2 MgCl2 (14040133, ThermoFisher Scientific). 200 000 T-ALL cells pre-stained with cell surface markers were incubated with this complexe ...
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bioRxiv - Genomics 2023Quote: ... were added into a fresh 2 mL microcentrifuge tube and washed with 1 mL of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Genomics 2023Quote: ... total RNA from young livers were spiked with 2 µl of a 1:100 dilution of ERCC Ex-fold Mix 1 while that from old livers were spiked with 2 µl of 1:100 dilution of ERCC Ex-fold Mix 2 (Thermo Fisher). Exfold ERCC spike-ins are provided in two mixes ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
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bioRxiv - Bioengineering 2024Quote: Coronal sections of healthy murine brain were prepared and stained as previously described101 either using 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng mL−1; Molecular Probes) to stain cell nuclei or primary antibodies for rabbit anti-NeuN (1:1000) ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 PierceTM protease inhibitor mini tablet/2 L; Thermo Scientific), sonicated ...