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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were passaged every 3-5 days at 70-80% confluence using 0.25% Trypsin-EDTA (Life Technologies). Cells were grown at 37°C in 5% CO2.
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bioRxiv - Molecular Biology 2024Quote: Total RNAs for 3 to 5 independent experiments performed in triplicate were extracted by Trizol (Life Technologies) and quantified on NanoDrop ...
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bioRxiv - Molecular Biology 2024Quote: ... Spike-in controls cel-miR-39-3p (C. elegans sequence: 5’-UCACCGGGUGUAAAUCAGCUUG-3’, Invitrogen, Waltham, MA, USA) and cel-miR-54-3p (C ...
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bioRxiv - Biochemistry 2024Quote: ... synthetic genes encoding the fusion proteins flanked by 5’-NdeI and 3’-XhoI sites were synthesized (Invitrogen GeneArt Gene Synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... A guide RNA targeting exon 41 of the LRRK2 gene (5’-CTCAGTACTGCTGTAGAATG-3’) was commercially synthesized (ThermoFisher) and used to form ribonucleoprotein (RNP ...
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bioRxiv - Biochemistry 2024Quote: ... and WNK1 (5’ CAGACAGUGCAGUAUUCACTT 3’) were transfected into HDMEC using Lipofectamine RNAiMax reagent (Thermo Fisher Scientific, 13778150). After 48 (TSC22D1 ...
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bioRxiv - Physiology 2024Quote: ... the cells were resuspended in 5 mL FACS buffer (PBS supplemented with 3% fetal bovine serum [ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... REV 5′ - GTG GAT AAC CCC TCC CCC AGC CTA GAC CA - 3′ provided by (Thermo Fisher Scientific (Waltham ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The 5 ‘and 3 ‘RACE were performed using the FirstChoice RLM-RACE kit (Ambion, Austin, TX, USA), according to Watanabe et al ...
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bioRxiv - Cell Biology 2024Quote: ... and position (1235-1252 bp) 5’ GcCGcATtCTcGAgGTtG 3’ (modified region corresponding to siRNA from Thermo Fisher s38908). The following restriction sites were modified ...
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bioRxiv - Cell Biology 2024Quote: ... and position (2164-2187 bp) 5’ gAgGAcGAgGCcTGGTAtCAaAaa 3’ (modified region corresponding to siRNA from Thermo Fisher s228498). The following restriction sites were modified ...
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bioRxiv - Cell Biology 2024Quote: ... and position (607-627 bp) 5’-cAaGAaTTcCGaGAgACtAAc- 3’ (modified region corresponding to siRNA from Thermo Fisher s34150). The following restriction site was modified ...
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bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
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bioRxiv - Neuroscience 2023Quote: ... were visualized using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43FX, a fixable analog of FM1-43 membrane stain, Thermo Fisher Scientific, Waltham, MA, USA). To stain the presynaptically active synapses of autaptic cultured neurons ...
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... UM-UC-3 cells were fluorescently labeled using 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI, ThermoFisher, #D3899) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate) (sulfo-NHS-SS-biotin) and streptavidin agarose were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg/ml L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1x antibiotic-antimycotic (Corning) ...
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bioRxiv - Microbiology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/mL sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) on ice for 45 minutes with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... and 0.1% L-(tosylamindo-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Affymetrix, Cleveland, OH, USA). Lastly ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/mL L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1× antibiotic-antimycotic60,61 ...
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bioRxiv - Biochemistry 2024Quote: ... The modified nucleosomes were then conjugated with PEAS [N-((2-pyridyldithio)ethyl)-4- azidosalicylamide] (Thermo Fisher), iodinated with [125I] and bound to saturating concentrations of WT or mutant Arp5 complexes ...
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bioRxiv - Biophysics 2024Quote: ... 1 μg/mL L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1× antibiotic-antimycotic (Corning) ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples (12 μL) were mixed with 3 μL 5 M DTT and 5 μL 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and incubated (95 °C ...
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 3.5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Microbiology 2024Quote: ... and their respective complemented strains cultured in 7H9 medium supplemented with 0.4% glycerol or sodium propionate were treated with CellROX Green (Life Technologies; final concentration, 5 μM) for 30 min at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM L-glutamine and non-essential amino acids (ThermoFisher Scientific), supplemented with 100 U/mL Il-2 (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM L-glutamine and non-essential amino acid (ThermoFisher Scientific). Both NK-92 and K562 cells were maintained in 37°C ...
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bioRxiv - Immunology 2023Quote: ... 2% MEM amino acids (from 50X stock; Gibco catalog number 11130051), 1% MEM vitamin solution (from 100X stock ...
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bioRxiv - Immunology 2022Quote: ... 2 mM L-glutamine and 90% non-essential amino acids (Gibco), 10% heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM Non-essential amino acids (Gibco, Cat No. 11140-035), and 0.1 mM 2-mercaptoethanol (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The suspension of pellet 2+3 was filtered through 40-μm cell strainer (Fisher scientific, Cat # 22363547), followed by final wash in complete Ham’s F10 medium ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were loaded onto a C18 trap column (3 μm, 75 μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Microbiology 2022Quote: ... All cells were passaged 2-3 times per week with 0.25% Trypsin/0.02% EDTA (Gibco Cat#25200056). Cells used in all experimental set-ups were between passage 5 and 30.