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Citations for Thermo Fisher :
1101 - 1150 of 7789 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA) and sequenced in both directions to confirm complete coding regions ...
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bioRxiv - Cell Biology 2023Quote: ... injection plasmids were miniprepped using PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher Scientific). Plasmids were combined at 100 ng/μl SEC plasmids and 50 ng/μl Cas9 plasmid and spun down (15,000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were harvested with a HiPure Plasmid Filter Midiprep Kit (Invitrogen, Cat #K210014). The library transfer reaction with LR Clonase II was carried out to move inserts into pDEST17 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were then collected for plasmid isolation (Thermo Scientific GeneJET Plasmid Miniprep Kit). Metagenomic inserts in the resistant hits were sequenced by two complementary sequencing methods ...
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bioRxiv - Physiology 2023Quote: ... isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific, Vilnius, Lithuania), and preparing plasmid standards to a known copy number ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were then extracted using the GeneJET Plasmid Mini-prep Kit (Thermo Fisher) and verified via Sanger sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmid DNA from positive clones was isolated (GeneJET Plasmid Miniprep Kit; Thermo Scientific) and Sanger-sequenced (Macrogen) ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA was purified using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and eluted in nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... V2LHS-196739 (shNCOR2-B)) and one non-silencing control construct were selected from the V2LHS pGIPZ based lentiviral shRNA library (Thermo Fisher Scientific). Viral packaging and cellular infection (RPCCC shRNA Resource ...
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bioRxiv - Cancer Biology 2022Quote: ... One million scramble/control or HIF1a targeting shRNA transduced SKOV3ip1 cells were stained using CellTracker™ Red CMTPX Dye (Thermo Fisher Scientific) and added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... For MDA-MB-231 with stable shRNA-mediated MCT4 KD (described in (Andersen et al., 2018)) the media was supplemented with 1 µg/ml puromycin (Gibco, #A11138-02). MCF10A cells were cultured in DMEM containing 4.5 g/l D-glucose ...
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bioRxiv - Cell Biology 2023Quote: ... and siRNA (corresponding to the shRNA sequences detailed below) were then introduced to the cells following the manufacture protocol (Thermo Fisher Scientific). The cells were left to incubate for 48 hours.
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bioRxiv - Cancer Biology 2023Quote: The shRNA constructs were ordered from Functional Genomics Unit (University of Helsinki) or designed by BLOCK-iT RNAi Designer (Thermo Fisher Scientific) and inserted into pLKO.1 Vector (Addgene) ...
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bioRxiv - Biochemistry 2024Quote: Lentiviral stable knockdown generation was done by growing HEK293T packaging cells in DMEM high glucose supplemented with 10% FBS and after 24 h were transfected with viral components and shRNA against target genes (or scrambled control) using Lipofectamine 2000 (Invitrogen, 11668–019), grown and concentrated using Lenti-X Concentrator (Clontech Laboratory ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... with a monoclonal antibody against B5 (19C2, rat, 1:1000; (Schmelz et al. 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer) prior to permeabilization of the cells with detergent ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were stained with a monoclonal antibody against B5 (19C2, rat, 1:1000 (Schmelz et al, 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer). Cells were then permeabilized with 0.1% Triton-X/PBS for 3 min ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were isolated from bacteria using the HQ PureLink Mini Plasmid Purification Kit (Invitrogen) with an extra wash step ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids from white colonies were purified using a GeneJet Plasmid Miniprep kit (Thermo Scientific) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids in this study were isolated using GeneJET Plasmid Maxiprep Kit (Thermo Scientific™).
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid was isolated from bacteria using the GeneJet Plasmid Miniprep kit (ThermoFisher; Cat#: K0502) or the GeneJet Plasmid Midiprep kit (ThermoFisher ...
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bioRxiv - Synthetic Biology 2021Quote: All expression plasmids were constructed from pENTR plasmids using Gateway cloning (Thermo Fisher Scientific). DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT ...
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bioRxiv - Microbiology 2021Quote: ... and a plasmid miniprep was prepared using GeneJET Plasmid Miniprep Kit (Thermo Scientific K0702). Plasmids were sequenced with both T7 promoter and terminator primers by the Eurofins A/S (Hamburg ...
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bioRxiv - Evolutionary Biology 2022Quote: ... plates were scraped into PBS and plasmid isolated (GeneJET Plasmid Miniprep Kit, Thermo Scientific). Primers encoding 8-bp 5’ and 3’ barcodes were used to amplify samples from each condition.
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bioRxiv - Microbiology 2022Quote: ... The plasmid DNA was isolated using the Gene Jet Plasmid Miniprep Kit (Thermo Scientific) and its sequence was validated by Sanger sequencing (GATC-biotech).
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bioRxiv - Bioengineering 2022Quote: ... Plasmid isolation was carried out using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... This viral plasmid and a plasmid expressing VSVG were mixed with lipofectamine 2000 (ThermoFisher) according to manufacturer’s instructions and were added to the cell culture ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid isolations were performed according to the GeneJET Plasmid Miniprep Kit protocol (Thermo Scientific) for low-copy-number plasmids ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2021Quote: ... then plasmid isolation was performed by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid DNA was isolated using PureLink® HiPure Plasmid Filter Maxiprep Kit (Invitrogen) with the last ethanol washes done in a sterile laminar-flow hood ...
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bioRxiv - Microbiology 2020Quote: ... and positive clones were grown for plasmid isolation (GeneJet Plasmid Miniprep Kit, Thermo Scientific). Sequence verified plasmids were then transformed into BL21(DE3 ...
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bioRxiv - Neuroscience 2022Quote: ... and plasmids purified using the PureLink™ HiPure Plasmid Filter Maxiprep Kit (Invitrogen, K210017), following manufacturer instructions.
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bioRxiv - Genomics 2022Quote: ... psPAX2 packaging plasmids and pMD2.G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific UK). The samples were analysed with quantitative real-time–PCRs (qRT–PCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... and their plasmids were prepared using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Once purified ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid DNA was extracted with the GeneJET Plasmid Miniprep Kit (Thermo Scientific, UK). Minipreps from P and P2 cells and from co-transformed strains were sent for Sanger sequencing (GATC ...
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bioRxiv - Microbiology 2022Quote: ... and PB2 expression plasmids were subcloned into the pFastbac Dual plasmid expression vector (Invitrogen) with polyhedrin promoter control ...
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bioRxiv - Developmental Biology 2023Quote: ... The final plasmids were purified using the GeneJET Plasmid-Midiprep-Kit (Thermo Scientific, K0481). All primers used are listed in Table S2.
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bioRxiv - Biophysics 2023Quote: ... The amplified plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: ... EGFP plasmid and plasmid/ASO transfection were carried out with Lipofectamine2000 (Thermo Fisher #11668030) following the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... transfected with the indicated plasmids (500 ng well; 250 ng/plasmid) using Lipofecatmine2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... All the plasmids were extracted using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and confirmed via Sanger sequencing performed by Eurofins Genomics.
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bioRxiv - Bioengineering 2023Quote: ... All the plasmids were extracted using a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific) and confirmed by Sanger sequencing by Eurofins Genomics.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA). Purified plasmids were sequenced in both directions to confirm coding regions.
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bioRxiv - Molecular Biology 2023Quote: ... these entry plasmids were recombined into mammalian destination plasmids using Gateway LR Recombination (Thermofisher) into pcDNA3.1 (Addgene #52535 ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA was isolated and purified using GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). All plasmids were confirmed by sequencing through Oxford Nanopore (Plasmidsaurus) ...