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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... SATO stock solution contained bovine serum albumin (BSA) fraction V (0.1 mg/mL; ThermoFisher Scientific), sodium selenite (4 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... 1% (v/v) penicillin-streptomycin and 1% (v/v) Glutamax (Gibco). For BJ fibroblasts ...
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bioRxiv - Neuroscience 2020Quote: ... primary DP cells from P5-8 Tgfbr2fl/fl molars were grown in alpha-minimum essential medium (alpha-MEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Genetics 2024Quote: ... The final digest was placed in a plastic cell culture dish with alpha modified Eagle’s medium (alpha-MEM) (Invitrogen) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... fetal liver cells were thawed and cultured for 48 h in alpha-minimum essential media (alpha-MEM) Glutamax (Gibco) containing 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Purified T cells were resuspended in AIM-V +5% SR (Gibco #A25961-01) and plated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Subsequent analysis was conducted using GeneMapper v.5 software (Applied Biosystems: Thermo-Fisher). Blood meals were screened for Plasmodium falciparum using a TaqMan quantitative PCR assay (26).
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bioRxiv - Neuroscience 2023Quote: ... and 5 μL of Alexa Fluor 647 Annexin V (Invitrogen, Thermo Fisher Scientific) diluted 1:10 with Annexin V binding buffer 1X were added to each 100 μl of cell suspension ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 μL of Alexa Fluor 647 Annexin V (Invitrogen, Thermo Fisher Scientific) diluted 1:10 with Annexin V binding buffer 1X were added to each 100 μl of cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Purified T cells were resuspended in AIM-V +5% SR (Gibco #A25961-01) and plated with Human T-Activator CD3/CD28 Dynabeads™ (Fisher Scientific (Thermo ...
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bioRxiv - Physiology 2020Quote: ... Eyes were decontaminated by submersion in 5% povidone-iodine ophthalmic solution (Betadine 5%, Fisher Scientific, NC9771653) for two minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 3X cholesterol or 5mM methyl beta cyclodextrin (MßC) (#377110050, Thermo Fisher) (doses chosen for biological effectiveness but minimal cellular toxicity [Supplementary Figures 2 and 3] ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with TBST + 5% nonfat dry milk then immunoblotted with anti-alpha-actinin 2 (Invitrogen EA-53; mouse monoclonal) and anti-actin (GeneTex HL1926 ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v) CO2 humidified atmosphere in Gibco®RPMI 1640 medium (Life Technologies) with GlutaMAX ...
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bioRxiv - Neuroscience 2021Quote: Primary neurons on cover glasses or 20-µm cryostat-sectioned coronal brain slices were incubated for 1 hour at room temperature with blocking buffer [PBS containing 5% (v/v) goat serum (#16210-064, Gibco, New Zealand), 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... all cells were grown under standard conditions (37°C, 5% (v/v) CO2) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific, Waltham, MA) containing 10% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... RPTEC-TERT cells were cultured at 37°C with 5 % (v/v) CO2 and humidity in DMEM/F12 (Fisher Scientific, 11330-032) supplemented with RPTEC growth kit (ATCC ...
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bioRxiv - Genomics 2024Quote: ... We transferred supernatants to new 1.5 mL tubes and precipitated RNA using 10% (v/v) 3M sodium acetate (pH 5.5) (cat. no. AM9740; Invitrogen, Waltham, MA, USA), molecular biology grade glycogen to a final concentration of 1 µg/µl (cat ...
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bioRxiv - Biophysics 2024Quote: ... The cell cultures were maintained within a controlled environment of a heated CO2 incubator at 37°C and 5% (v/v) CO2 concentration (Forma Steri-Cycle CO2 incubator, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... were separated over a 30 min linear gradient of 5–45% (v/v) acetonitrile on a Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 µm x 50 cm) (Thermo Scientific). MS1 spectra with a mass range of m/z 200– 2000 were acquired at 3 Hz and the 18 most intense peaks were selected for MS/MS analysis with an intensity-dependent spectrum acquisition rate between 4 and 16 Hz ...
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bioRxiv - Cell Biology 2022Quote: ... followed by trypsin (Thermo Fisher Scientific, 1:50 w/w protein) overnight at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... at 1:1 ratio (w/w DNA:PEI) in Opti-MEM (Gibco). Media was changed to complete DMEM (10% v/v FBS ...
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bioRxiv - Bioengineering 2023Quote: ... and polylysine (90%:10% w/w ratio respectively; Fisher Scientific, ICN19454405) were immobilized on concentrated gold nanoparticles and incubated overnight ...
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bioRxiv - Bioengineering 2023Quote: ... 23% (w/w) fusidic acid (FA; 98%, 5552333, Thermo Scientific Acros) in PLA was incorporated (FA/PLA total content of 10% w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultivated into cerebral organoid differentiation medium (50% [v/v] DMEM/F12 with 50% [v/v] Neurobasal [Gibco Cat#21103049] containing 1% [v/v] Glutamax [Gibco Cat#35050061] ...
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bioRxiv - Cancer Biology 2022Quote: ... 10% (v/v) ammonium persulfate and 0.1% (v/v) TEMED) (Thermofisher,17919) combined with 5% stacking gel ...
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bioRxiv - Microbiology 2020Quote: ... and were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2% (v/v) fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific, Waltham, MA, USA) at 37°C in a 5% humidified CO2 incubator ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked in 1% (w/v) BSA and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in 1% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with HRP-conjugated secondary antibody in 4% (w/v) skimmed milk in TTBS and visualized using SuperSignal West Pico PLUS (Thermo Fisher Scientific).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The plasmodia were cultured on 150mm cell culture dishes covered in a layer 25mm thick of 1% w/v agar (Fisher Scientific, USA). Cultures were maintained in a temperature-and humidity-controlled (90% humidity and 22 °C ...
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bioRxiv - Microbiology 2021Quote: ACPL-GFP D10 and NF54 PfMev parasites were synchronized with 5% (w/v) D-sorbitol for 10 minutes at room temperature and returned to culture in 10 µM fosmidomycin (Invitrogen Life Technologies F23103), 100 nM atovaquone (Caymen Chemicals 23802) ...
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bioRxiv - Bioengineering 2021Quote: ... 0.05% (w/v) Tween-20) and incubated with a 10-fold dilution of Blocker bovine serum albumin (BSA; Thermo Fisher Scientific 37525) in wash buffer for 1 h at 25 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were blocked in 1% (w/v) BSA and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in 1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The digested product was then electrophoresed on 1% (w/v) agarose gel and purified from the gel slice using PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific). The purified product was subcloned into pACYC177 plasmid at DraIII-XhoI sites ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times in warm PBS and fixed for 45 min at room temperature in 4% (w/v) formaldehyde (Thermo Scientific; 28908) in PBS containing 0.1% Triton-X100 ...
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bioRxiv - Plant Biology 2022Quote: ... The specificity of the primers was further validated using PCR and DNA gel electrophoresis on 1% (w/v) agarose gels containing 1X SYBR Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific) in 1X TAE buffer ...
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bioRxiv - Developmental Biology 2022Quote: Elastomeric stamps containing the desired geometries in bas-relief were coated with bovine serum albumin in PBS (1% w/v in PBS; Thermo Fisher Scientific) for overnight to prevent hydrogel adhesion ...
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bioRxiv - Bioengineering 2020Quote: ... samples were fixed with 4% paraformaldehyde (w/v) in PBS for 1 h and stained with Actin Red 555 (Molecular Probes, ThermoFisher) for 1h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... prepared as a 2.3 mM stock in dimethyl sulfoxide (DMSO) and mixed in a 1:1 ratio with 10% (w/v) pluronic acid F-127 (Invitrogen, Carlsbad, CA) and diluted in Assay Buffer (Hank’s Balanced Salt Solution (HBSS) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1 % (w/v) sodium dodecyl sulphate (SDS)) with 1x Halt™ Protease and Phosphatase Inhibitor Cocktail with EDTA (Pierce, Thermo Fisher Scientific). Protein samples were prepared in 4x NuPAGE LDS Sample Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Neuroscience 2021Quote: ... Immediately prior to imaging sessions the vascular lumen was labelled either by intravenous injection with 2.5% (w/v) Texas Red Dextran (70 kDa, Invitrogen, Carlsbad, CA, USA) or subcutaneous injection of 2.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then negatively stained for 1 min using 10 μl of 2% (w/v) uranyl acetate and examined with a Cryo-TEM (FEI Tecnai G2 Spirit Twin) (Thermo Fisher Scientific) using an accelerated voltage of 120 KV ...
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bioRxiv - Genetics 2020Quote: ... Confocal microscopy was carried out embedding the 6 dpf larvae in 1 % (w/v in E2 medium containing tricaine) low-melting agarose (Fisher Scientific, USA) in a 50-mm glass bottom dish (MatTek ...
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bioRxiv - Developmental Biology 2022Quote: ... oocytes were transferred to 0.1% (w/v) polyvinylpyrrolidone (PVP) in PBS containing 10 μg/ml of Hoechst 33342 (Life Technologies, Ghent, Belgium) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... planktonic media was carefully removed and washed twice with 0.1% saponin (w/v%) and incubated with FilmTracer™ LIVE/DEAD™ Biofilm Viability Kit (Invitrogen, USA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and a 100 μg protein sample was separated on a 10% (w/v) PAGE and visualized by staining with Coomassie Brilliant Blue or immunoblotted with anti-Ub (Invitrogen; pa1-10023).