Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 7 METHYLIMIDAZO 1 2 B 1 2 4 TRIAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... amphotericin B (1:166; 250μg/ml, Gibco), gentamicin (1:1000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... amphotericin B (1:166; 250μg/ml, Gibco), gentamicin (1:1000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... amphotericin B (1:166; 250μg/ml, Gibco), gentamycin (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... and 1% amphotericin B (Thermo Fisher Scientific). The mock-treated ...
-
bioRxiv - Immunology 2023Quote: ... 1× B-27 (ThermoFisher Scientific 50X, 17504044), and 1× N-acetyl-L-cysteine (Sigma ...
-
bioRxiv - Bioengineering 2023Quote: ... with 1:50 B-27 supplement (Gibco), 1:100 GlutaMAX supplement (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1× B-27 supplement (17504044, Gibco)) supplemented with EGF (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 ml B-27 supplement (50x, Gibco), 5 ml R-spondin ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 ml B-27 supplement (50x, Gibco), 100 µl Primocin (50 mg/ml ...
-
bioRxiv - Developmental Biology 2024Quote: ... with 1× B-27 supplement (Gibco, 17504044) and 0.5 mM L-glutamine were tested ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
-
bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
-
bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then loaded with 3μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) (Thermofisher, D399) for 20 minutes and incubated at 37°C in a humidified incubator containing 5% CO2 ...
-
bioRxiv - Cell Biology 2019Quote: ... the carboxylated analog of the cell-permeant agent 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Thermofisher) was used ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA) (10µM; Invitrogen, Carlsbad CA) 4 hours before harvest ...
-
bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
-
bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
-
bioRxiv - Microbiology 2021Quote: ... and mixed 1:1 with B-PER reagent (Thermo Fisher Scientific). Cells were lysed by three passages through a French press at 138 MPa pressure ...
-
bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1/1000 b-mercapto-ethanol (Life Technologies) in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each ...
-
bioRxiv - Immunology 2022Quote: ... containing 4% B-27 (Life Technologies), 30 μM ascorbic acid (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4% B-27 supplement (Thermofisher Scientific), 30 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) ...
-
bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
-
bioRxiv - Neuroscience 2021Quote: ... fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into VLS ...
-
bioRxiv - Neuroscience 2021Quote: ... in Neurobasal medium enhanced with 2% B-27 and 0.5% L-Glutamine (Life technologies). Primary hippocampal neurons growing on either 12mm or 18mm coverslips ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
-
bioRxiv - Neuroscience 2020Quote: Fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into V1 (AP ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
-
bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
-
bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
-
bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
-
bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
-
bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
-
bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
-
bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
-
bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
-
bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
-
bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
-
bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...