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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 4°C for 45 min with 2 μg/mL Alexa647 (Life Technologies)-conjugated M1 anti-FLAG (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, in blue, Molecular Probes, Eugene, OR). The slides were observed with a confocal microscope (Fluoview FV100 ...
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bioRxiv - Cancer Biology 2022Quote: ... SlowFade™ gold antifade mountant containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) was applied to stain nuclei and reduce photobleaching ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was visualized using 1 µg ml-1 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen). Acrosomes were visualized using 0.5 µg ml-1 lectin peanut agglutinin (PNA ...
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bioRxiv - Bioengineering 2023Quote: ... 50 mg Sulfo-SANPAH (sulfosuccinimidyl 6-(4’-azido-2’- nitrophenylamino)hexanoate) (cat. 22589, ThermoFisher Scientific) was dissolved in 1 ml DMSO and diluted 25-times in Milli-Q water ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI, 5µg/ml, DAPI, ThermoFisher D1306) in PBS for 5 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were mounted with ProLong diamond with 4’,6’-diamidino-2-phenylindole (DAPI) (P36931; Invitrogen) and imaged on an Axio Observer 7 equipped with an Axiocam 702 mono camera and Zen 2.6 Pro software (Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated 2-4 hrs in Earle’s balanced salt solution (EBSS; Gibco, # 24010043). Bafilomycin A1 (referred as BafA1 ...
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bioRxiv - Cell Biology 2024Quote: ... Either 4 nM WR99210 (Jacobus Pharmaceuticals) or 2 μg/mL blasticidin S (Life Technologies, USA) was used for selecting transfectants ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of 5M NaCl and 2 µl of RNaseA (10 mg/mL) (ThermoFisher, # EN0531) were added to each sample and the samples were then incubated at 65°C with shaking overnight.
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bioRxiv - Immunology 2024Quote: ... and stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62247) for 2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex (2-4 µM range) was initially imaged in a Talos L120C (Thermo Fisher) electron microscope equipped with a LaB6 electron source and a Ceta-M camera ...
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bioRxiv - Biochemistry 2024Quote: ... and 10% 2-mercaptoethanol and run on NuPAGE 4-15% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Immunology 2024Quote: ... Tissue blocks were counterstained with DAPI (4’, 6-diamidino-2-phenylindole, Dihydrochloride) (Invitrogen, Waltham, MA) and cover slips placed using Vectashield Mounting Media Hardset (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis was evaluated thanks to 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, USA) and Annexin-V-APC (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; #D3571, Invitrogen) then mounted on glass slides with Mowiol ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) for nuclear staining (H3570; Invitrogen; dilution 1:200), diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear counterstaining was performed with 100 ng/ml DAPI (4’,6-diamidino-2-phenylindole; Invitrogen) solution in PBS for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... to visualize actin filaments and with 4’-6-diamidino-2-phenylindole (DAPI, Invitrogen, 1:200) for the nucleus ...
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bioRxiv - Cell Biology 2024Quote: ... mice were injected intraperitoneally with 4-ethynyl-2’-deoxyuridine (EdU; E10187; Invitrogen, Waltham, MA, USA) at 10mg/kg to assay cellular proliferation ...
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bioRxiv - Cell Biology 2023Quote: A two-step RNA isolation protocol was followed for total RNA extraction: Organic phase isolation was performed with TriReagent®/ bromo–3–chloropropane (BCP) (Thermo Fisher Scientific/Molecular Research Center ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected retinas were either fixed in 4% formaldehyde (MilliporeSigma, Cat.# FX0415-4) in PBS for 2 hours at room temperature or lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were grown exponentially in an incubator at 37°C under 5% CO2 and subcultured at ratios of 1:2-1:10 every 2 to 4 days using 0.05% trypsin-EDTA (Thermo Fisher Scientific, Cat# 25300120).
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: Larvae were immersed in 100 μg/mL 5-ethynyl-2′-deoxyuridine (EdU) solution (A10044; Invitrogen) for 16 h before termination of the experiment ...