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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then passaged 1:3-1:6 every 2-3 days using Accutase (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Biophysics 2020Quote: ... The culture medium was replaced with fura-2 AM (6 μM, F1221; Thermo Fisher Scientific) in Opti-MEM (11058-021 ...
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bioRxiv - Microbiology 2022Quote: ... 6-Diamidino-2-Phenylindole (DAPI, Ex 360 nm and Em 460 nm, Thermo Fisher, D1306) or (SYBR Green I ...
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bioRxiv - Developmental Biology 2020Quote: ... Discs were then incubated with 2.5 μg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in PBS + 0.1% Tween-20 for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Neuroscience 2022Quote: ... A nuclear counterstaining was performed using 4ʹ,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) in TBS for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 6 pmol of siRNA (siCHC-1: Silencer Select s475, siCHC-2: s477 Thermo Fisher 4390824) were mixed with 100 µl of OptiMEM reduced serum media (Thermo Fisher 31985070 ...
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bioRxiv - Microbiology 2024Quote: ... and stained with 2-(4amidinophenyl)-1H-indole-6-carboxamidine (DAPI, 1 μg/mL; Invitrogen, D1306) and washed with Dulbecco’s PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections were stained with hematoxylin and eosin (H&E) or mounted with 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (Thermo Fisher Scientific, Waltham, MA, USA). They were imaged with SLIDEVIEW VS200 Slide Scanner (Olympus ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues were stained with DAPI (4′,6-diamidino-2-phenylindole) for 5 minutes and mounted in ProLong™ Diamond Antifade Mountant (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Cell Biology 2021Quote: 1.5×104 cells were seeded in one well of a 6-well plate (Falcon) with growth medium comprising of DMEM/F-12 (Gibco) with 20% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ESCs in one well of a 6-well plate were transfected with 10 μg of expression vector using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: 1×105 N2A or HEK293T (ATCC) cells were seeded in one well of a 6-well plate in 2mL of DMEM complete media (Gibco) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Neuroscience 2024Quote: 3 to 6 dpf-zebrafish larvae were placed in a 48-well plate (one larva per well, Fisher Scientific, USA) filled with embryo medium ...
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bioRxiv - Zoology 2024Quote: ... One well was loaded with 6 µL of a protein standard (SpectraTM Multicolor Low Range Protein Ladder; Thermo Fisher Scientific). The peptides were separated in the gels by SDS- PAGE and subsequently transferred to PVDF membranes with 0.2 μm pore size (Carl Roth GmbH & Co ...
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bioRxiv - Cell Biology 2024Quote: ... or from ventricular tissues of various mouse hearts was minced and placed into one well of 6-well non-coated plate and cultured in 3 ml MSC medium with Normocin (Invitrogen) and Gentamicin (GIBCO).28 The media was changed daily for 3 days and then every 2-3 days ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...
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bioRxiv - Immunology 2021Quote: ... 5/6 weeks and 6/7 weeks post-infection by flow cytometry using counting beads (CountBright, ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... 6-carboxyfluorescein (FAM)-5= CCG TCA ATC AAG GAG CGC CTC 3=-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... 6-carboxyfluorescein (FAM)-5’ CCG TCA ATC AAG GAG CGC CTC 3’-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 6 ml liquid MSgg medium of each well of a 6-well microplate (Thermo Scientific), and then grown for additional four days ...
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bioRxiv - Microbiology 2020Quote: ... 6 × 105 293LTV cells were seeded in a 6-well plate and transfected using Lipofectamine 2000 (Invitrogen) which was complexed with DNA plasmids driving the expression of either VSV G protein (positive control) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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bioRxiv - Cell Biology 2023Quote: ... Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen). Constructs were bombarded into unc-119(ed3 ...
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Biochemistry 2023Quote: ... The sample was then pegylated for 15 minutes with 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) before grids.
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bioRxiv - Biophysics 2021Quote: ... Methyl-PEG4-thiol (MT(PEG)4) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 × 105 cells were seeded in 6-well plates and cultured with RPMI 1640 medium (ThermoFisher # 11875093) containing 10% FBS (Seradigm) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines were passaged every 4-6 days and MIN6 were detached with 0.25% trypsin-EDTA (Gibco) and seeded at 7.5-1×106 cells per 75 cm2 flask ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were passaged every 4 to 6 days by dispersion with TrypLE Express (Invitrogen, Carlsbad, CA, USA) and re-embedded in Matrigel-GFR.
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bioRxiv - Bioengineering 2024Quote: ... This was followed by foregut cell differentiation from day 4 - 6 using advanced DMEM/F12 (Gibco; 12634) with 2% B27 (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were then treated with 4-6 units of Turbo DNase (Thermo Fisher Scientific, cat# AM2238) at 37 °C for 30 min to remove the bulk of plasmid DNA contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... aegypti embryos at specified developmental stages (0-1h, 4-5h, 5-6h, 23-24h, 47-48h, and 71-72h) using Trizol® reagent (Ambion by Life Technologies), following the manufacturer’s instructions ...