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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... EdU staining was performed 24 hours later using the EdU ClickIt Reaction Kit (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: 293T cells were cultured in 24-well plates (NEST) in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal calf serum (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... DNA is then isolated using phenol-chloroform:isoamyl alcohol (25:24:1) (Thermo Fisher Scientific, 15593031) as previously described (Wu et al. ...
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bioRxiv - Microbiology 2023Quote: ... transferred into a solution consisting of phenol:chloroform:isoamyl alcohol (PCI, 25:24:1, pH 7.9, Ambion) and 20% sodium dodecyl sulfate in Tris-EDTA buffer with sterile 0.1 mm glass beads (BioSpec Products #11079101) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were collected every 24 hours stained with NucBlue™ Live Cell Stain (ThermoFisher LTD), according to manufacturer protocol and analyzed using LSRII cell analyzer plate reader ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA extraction followed using the phenol-chloroform-isoamyl alcohol 25:24:1 (PCI; Invitrogen, 15593049) method as described in Skene P et al ...
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bioRxiv - Cell Biology 2022Quote: ... UK) were maintained in DMEM supplemented with 2 mM L-glutamine (ThermoFisher Scientific, 25030-24), non-essential amino acids (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... at a 2:1 ratio in a 24 well plate in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2022Quote: ... samples were kept at −80°C for 24 hours and 1 μl GlycoBlueTM Coprecipitant (ThermoFisher) was added ...
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bioRxiv - Evolutionary Biology 2022Quote: ... they were transferred individually into wells of Falcon® 24-well plates (Thermo Fisher Scientific), and developmental progress through the copepodid stages (C1 ...
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bioRxiv - Physiology 2022Quote: ... Nasal ALIs were washed 24 hrs prior with antibiotic-free Ham’s F12K media (ThermoFisher Scientific) on the basolateral side ...
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bioRxiv - Neuroscience 2022Quote: ... the hippocampal slice was placed in a well of a 24-well culture plate (Nunc) and 10 μg plasmid DNA in a volume of 25-30 μL of AD1 Solution (Lonza Bioscience ...
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bioRxiv - Biophysics 2023Quote: tsA201 cells were plated in 24-well plates and transfected using Lipofectamine 2000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Transient transfection was conducted 18-24 h before patch-clamp recording using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 24 h in serum-free medium (SFM, Gibco, Thermo Fisher Scientific) supplemented with 100 U/mL penicillin ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 24 h in serum-free medium (SFM, Gibco, Thermo Fisher Scientific) supplemented with 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2023Quote: ... and/or collagen coated 6- or 24-well plates (145380 or 142475, Thermo Fisher Scientific), and cultured at 37°C and 5% CO2 in DMEM/F12 (21041-025 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected 24 h later with a mixture of 8 μl of Oligofectamine (Invitrogen) and the indicated siRNA to a final concentration of 100 nM ...
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bioRxiv - Neuroscience 2024Quote: ... Cell viability was measured after 24 hours using PrestoBlue Cell Viability Reagent (Thermo Fisher Scientific). Treatment media was replaced with 1X PrestoBlue reagent diluted in phenol red-free media ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acids were extracted with equal volume of ultrapure phenol:chloroform:isoamylic alcohol (25:24:1) (ThermoFisher) followed by extraction with chloroform alone ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene expression was assessed as previously described [24] using ABI TaqMan commercial primers (Applied Biosystems). Results were expressed using mouse PPIB as a reference gene.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1,5 × 106 PBMCs were seeded per well in a 24-well plate (10380932, Fisher Scientific) in RPMI-1640 supplemented with 10% FBS (11573397 ...
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bioRxiv - Biochemistry 2024Quote: ... An equal volume of phenol/chloroform/isoamyl alcohol (25/24/1, pH 6.6, LIFE TECHNOLOGIES) was added to this mix ...
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bioRxiv - Biochemistry 2024Quote: ... 800 s by 10 μL phenol-chloroform-isoamyl alcohol (25:24:1 v/v, Invitrogen). Following extraction of the aqueous layer ...
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bioRxiv - Biophysics 2024Quote: ... grown over night and transiently transfected for 16–24 hours with plasmids using TurboFect (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: 24-well plates were coated with 500 µl of Collagen I per well (ThermoFisher, A1048301) and incubated for 3h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Corning Co-star 24-well tissue culture (TC) plates (Thermo Fisher Scientific, cat. no. 353047)
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bioRxiv - Cancer Biology 2024Quote: ... 5,000 cells were seeded into each well of a 24-well plate (Fisher Scientific, FB012929). Cells were harvested at the indicated time points by trypsinization ...
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bioRxiv - Cell Biology 2024Quote: ... BOECs were directly seeded in 24-well plates with a glass bottom (Thermo Fisher Scientific). After two hours ...
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bioRxiv - Cancer Biology 2020Quote: ... MS-5 mesenchymal cells were grown in α-MEM medium w/o nucleosides (Gibco) supplemented with 10% fetal calf serum ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Neuroscience 2024Quote: ... NIM was replaced to 3:1-medium consisting of 3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... cells were stained with DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (DiI, Invitrogen cat # D383) for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Cell Biology 2024Quote: ... and biotinylated at the 3’end with the Pierce RNA 3’end biotinylation kit (Thermo Fisher). Both antisense and sense strands of the DNA sequence were amplified to obtain a DNA template for in-vitro transcription (Supplementary Figure ...
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bioRxiv - Developmental Biology 2024Quote: 3 dpf larvae were incubated in 3 µM FM™ 1-43 Dye (Thermofisher catalog # T3163) for 2-4 minutes ...
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bioRxiv - Molecular Biology 2024Quote: Human fibroblasts were seeded in a 24-well plate and stimulated with tumor necrosis factor-α (TNF-α) (5 ng/ml; Life Technologies, Landsmeer, the Netherlands) plus lymphotoxin α1β2 (50 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Cell Biology 2023Quote: ... All drug treatments were performed for 3 – 5 hours prior to imaging with a final concentration of 5 nM of actinomycin D (Gibco, 11805-017) or 1 µM of BMH-21 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 uM FluoZin-3 AM (Invitrogen) was add to dishes to stain cells for 1 hour and then was washed twice with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 5X SSCT (ThermoFisher Scientific 15557044 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-phosphohistone 3 (1:100, Invitrogen), anti-phosphoAMPK (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 3 mM MgCl2 (Thermo Fisher AM9530G), 20 mM Tris-HCl pH 7.5 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... TO-PRO-3 (Thermo Fisher Scientific), or Hoechst 33258 in the blocking buffer for 60 min.