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Citations for Thermo Fisher :
1051 - 1100 of 6563 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Parental T-REx-HEK293 cells were cultivated in growth medium: Dulbecco’s modified Eagle’s medium (DMEM) + GlutaMAX-I (#31966, Life Technologies), 10% Dialyzed FBS (Gibco ...
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bioRxiv - Bioengineering 2022Quote: Flasks of HEK293 cells were pre-labelled red or green using cell tracker labels (C7025 and C34552, Thermofisher Scientific) or blue using Hoescht 33342 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were maintained in HEK293 culture medium – Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate; Gibco, #41966029) with 10% foetal calf serum (FCS ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Synthetic Biology 2023Quote: ... Hek293-T cell lines were cultured in DMEM (ATCC; 30-2002) supplemented with 10% FBS (Thermo Fisher Scientific; 10082147), 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cell Biology 2023Quote: ... wild type HEK293 or HEK293A (for figure 1A) cells were transfected with the indicated plasmids with Lipofectamine2000 Reagent (Invitrogen) as per manufacturer protocol ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: NIH3T3 derived Bmal1-luc31 and HEK293/T17 cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Gibco: Cat #11965118) supplemented with 10 % fetal bovine serum (Corning ...
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bioRxiv - Immunology 2024Quote: ... HEK293T TRIM21 knock-out and HEK293 HOIP KO cells were maintained at 37°C in complete DMEM (ThermoFisher 10569010) supplemented with 10% FBS and pen/strep.
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... Stable cell lines were made by transfecting HEK293 cells with the respective plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Developmental Biology 2021Quote: ... C2cd6s was subcloned into pcDNA3.1/myc-His A vector (Cat. No. V800-20, Invitrogen). The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... using the Ion PI™ Hi-Q™ OT2 200 kit (Invitrogen; Cat #A26434) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... incubated with DPBS containing 10% HI FBS and 1:1000 Hoechst 33342 (Invitrogen, #H3570) for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum [HI-FBS] (Cat#SH30071.03, Thermo Scientific), penicillin [100 IU/ml]-streptomycin [100 ug/ml] (Quality Biological ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC DNA extraction was performed using Hi-Pure Plasmid DNA Extraction Kit (Invitrogen K210017). Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910) ...
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bioRxiv - Molecular Biology 2019Quote: ... full-length MmEsco2 was cloned into the pcDNA3.1/myc-His vector (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Immunology 2021Quote: ... The extracellular domain of NKp46 was cloned into pcDNA Myc-His 3.1a vector (Invitrogen) or pCMV vector (Addgene plasmid #59314 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was incubated with 1mL His-Pur nickel-NTA resin (Thermo Fisher) with rotation at 4 °C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... ZAP-S and ZAP-L were subcloned into pEF1-V5/His (Thermo Fisher Scientific) via the KpnI/XbaI sites to generate pEF1-ZAP-S-V5/His and pEF1-ZAP-L-V5/His ...
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bioRxiv - Neuroscience 2022Quote: ... the pEx-FGD4-His-V5 vector was generated by Gateway cloning technology (Thermofisher, USA). Transfection experiments were performed using promofectin reagent (#PK-CT-2000-50 ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Immunology 2022Quote: ... unbiotinylated form were incubated with 2μg/mL anti-His biotin (Invitrogen, MA1-21315-BTIN) for 20 min at room temperature before being used to label the streptavidin beads ...
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...