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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 6 Methoxy 2 methyl 1H benz de isoquinoline 1 3 2H dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Bioengineering 2022Quote: ... 1% N-2 (Thermofisher), 2% B-27 (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... 1× N-2 (Gibco), 50 ng/ml of mouse EGF (RnD) ...
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bioRxiv - Cell Biology 2020Quote: ... incubated 2-3 minutes with SuperSignalTM West Pico Chemiluminiscent Substrate (Thermo Scientific) and imaged using C-DiGit® Blot Scanner (LI-COR).
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bioRxiv - Biochemistry 2020Quote: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Thermo Fisher Scientific) was added to cells at a final concentration of 2mM and incubated at 37°C in 5%CO2 for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... A predesigned TaqMan assay targeting exon 2–3 boundary (Hs00213726_m1; Life Technologies) was also used to ensure equal amount of the endogenous A4GALT transcript in transfected and non-transfected cells ...
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bioRxiv - Cell Biology 2021Quote: ... Jade1/2/3 mRNA was assessed by SYBR Green (Thermo Fisher Scientific) qPCR using Hprt1 as endogenous control ...
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bioRxiv - Cell Biology 2021Quote: ... and were passaged every 2 or 3 days using 0.05% Trypsin (Gibco). mESCs were used at passages below 30 ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were fed every 2-3 days with DMEM (ThermoFisher Scientific, #11995073) media supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were passaged once every 2-3 days by trypsinization (Gibco, 25300054) upon reaching ∼70-80% confluency ...
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bioRxiv - Physiology 2020Quote: ... for 3 days with macrophage-conditioned medium containing 2% Horse Serum (Gibco). Cells were washed ...
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bioRxiv - Cell Biology 2019Quote: ... which were activated through consecutive incubation with 2% 3-aminopropyltrimethoxysilane (Acros Organics) in isopropanol for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... spiked with 2-3 μL Plus reagent (15338100, Thermo Fisher Scientific, USA). The plasmid solution was mixed and incubated for 10 min at room temperature ...
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bioRxiv - Pathology 2022Quote: ... 2 and 3 were quantified using CyQUANT Proliferation Assay Kit (Invitrogen, C35011); For myofibroblast differentiation assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Dynabeads with precipitated proteins were washed 3 times with DynaMag-2 (Invitrogen) and eluted with LDS buffer (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... This medium was exchanged 2-3 hours after plating with Neurobasal (Gibco) supplemented with L-glutamine (2 mM) ...
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bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were split every 2 to 3 days using TrypLE Express (Gibco).
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bioRxiv - Immunology 2023Quote: ... 3) Dyes: NucBlue Live Cell Stain (2 drops/ml, R37605, Molecular Probes), phalloidin-568 (A12380 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: Cells were passaged every 2-3 days by incubation with Accutase (Gibco) for 2-3 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and TaqMan assay reagents (Table 2) on the QuantStudio 3 (Applied Biosystems). Gene expression was normalized to GAPDH expression ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Immunology 2020Quote: ... the cells were stained for 1h with the LIVE/DEAD™ fixable near-infrared dead cell stain (1:1000 dilution, Invitrogen) along with the following labelled antibodies diluted in an ice-cold staining buffer (PBS supplemented with 1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... The following secondary antibodies were added for 1h at room temperature: anti-rabbit IgG-647 (1:500, Life technologies, A-21244), anti-rabbit IgG-488 (1:500 ...
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bioRxiv - Pathology 2020Quote: ... (Extended Data Table 1) and corresponding fluorescently conjugated secondary antibodies (1h at RT) together with 10 μg/ml Hoechst 33342 (trihydrochloride, trihydrate, ThermoFisher Scientific). Sections were mounted with ProLong Gold Antifade mounting medium ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed and incubated for 1h at RT with 1:200 Alexa-fluor secondary antibodies: goat anti-rabbit 488 (Life Technologies), goat anti-rat 568 (Life Technologies ...
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bioRxiv - Physiology 2023Quote: ... Freshly dissected SCG were digested 1h at 37°C in trypsin 1X (Trypsin 10X, Life technologies, diluted 1:10 in DPBS). Sympathetic neurons were dissociated ...
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bioRxiv - Cancer Biology 2022Quote: ... Novusbio # NBP1-78278) was incubated for 1h at room temperature and revealed with a Pacific Orange-conjugated secondary antibody (1:50, ThermoFisher, # P31584). After DAPI counterstaining ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed and then incubated with the corresponding secondary antibodies for 1h at room temperature (Goat Anti-rabbit Secondary Antibody, HRP conjugate 1:1000, ThermoFisher #31460). All antibodies were diluted in a 5% BSA solution in TBS-T ...
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bioRxiv - Genetics 2024Quote: ... samples were washed three times with PBS and incubated for 1h with the secondary antibodies Alexa Fluor 647-conjugated chicken anti-rabbit (1:200, #A21443, Life Technologies), Alexa Fluor 488-conjugated goat anti-mouse (IgG2a ...
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bioRxiv - Neuroscience 2023Quote: ... the cells incubated with secondary antibodies in blocking solution for 1h at RT in the dark (anti-chicken Alexa 568, 1:1000, A11041, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... After washing the coverslips with PBS, samples were incubated with appropriate secondary antibodies (1:1000, 1h at room temperature) from Thermo Scientific: goat α-rabbit combined with Alexa-405 (#A-31556) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated for 2h at RT with the secondary antibodies: goat anti-mouse IgG Alexa Fluor 488 (Thermo Fisher, A31560, 1:500) and goat anti-rabbit IgG Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... To-Pro-3 (1:500, Invitrogen T3605). Secondary antibodies were obtained from Jackson ImmunoResearch or Invitrogen and used at 1:200.
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bioRxiv - Cell Biology 2019Quote: RNF168 si #1: 5’- GGCGAAGAGCGAUGGAGGATT-3’ (Ambion)
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bioRxiv - Biochemistry 2022Quote: ... 3) 1% Pluronic-127 (cat. # P6866, ThermoFisher) in BRB80 ...
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bioRxiv - Systems Biology 2022Quote: ... diluted 1:3 with DPBS (Life Technologies) and 25μL added to the wells ...
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bioRxiv - Bioengineering 2021Quote: ... + 1 × 10−3 M sodium pyruvate (Gibco) + 0.05 × 10−3 M 2-mercaptoethanol (Sigma)] supplemented with soluble anti-mouse CD28 (5 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and Toto-3 (1:1000; Thermo Fisher) as nuclear counterstain.
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bioRxiv - Immunology 2022Quote: ... ICOS-SB436 (ISA-3, Invitrogen, 1:50), IgG-BV480 (goat polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... or TO-PRO-3 (1:50000, ThermoFisher) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... proteinase K (3 μg ml−1; ThermoFisher) digestion ...
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bioRxiv - Immunology 2023Quote: ... claudin-3 (1:200; Thermo Fisher Scientific), claudin-15 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-3 L of Expi293F cells (ThermoFisher) were grown at 37°C to a cell density of 3.2×106 cells/mL in FreeStyle expression medium (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: ... and TO-PRO-3 (1:5000, ThermoFisher). The combination of Nucred Dead 647 and TO-PRO-3 has previously been described by Dekkers et al24 ...