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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... loaded into a NuPage 8% Bis-Tris Midi Gel (Invitrogen) in 1x MOPS SDS Running Buffer (Novex) ...
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bioRxiv - Neuroscience 2023Quote: ... 8-10 fields of TetraSpeck beads (100 nm; Invitrogen T7279) immobilized on coverslips coated with poly-L-lysine were imaged for 100 frames with 50 ms exposure and low laser power for determining dual-view correction.
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bioRxiv - Immunology 2023Quote: ... hTLR7/8 dual-agonist resiquimod (R848, Cat#tlrl-r848, Invitrogen) was used in cell studies at the working concentration of 1 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... medium supplemented with 8 mM L-Glutamine (ThermoFisher Scientific, 25030081) and 0.1% Pluronic-F68 (ThermoFisher Scientific ...
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Glioblastoma cells use an integrin- and CD44-mediated motor-clutch mode of migration in brain tissuebioRxiv - Cancer Biology 2023Quote: ... supplemented with 8% fetal bovine serum (FBS; 10438-026; Gibco) and 1x penicillin/streptomycin (100 IU/mL Penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 8-10 μm sections on SuperFrost Plus slides (Thermo Scientific) were fixed in 1% paraformaldehyde for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... hTR-8/SVneo cells were maintained in RPMI 1640 (Invitrogen) supplemented with penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... 8 % CO2 in DMEM containing HAT supplement (21060-017 Gibco). After 10 days in culture ...
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bioRxiv - Cell Biology 2024Quote: ... in an 8-well live imaging dish (LabTekII, Thermo Fisher) coated with fibronectin ...
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bioRxiv - Bioengineering 2024Quote: ... 8-well chambered coverglasses (Nunc Lab-Tek II, Thermo Scientific) were coated with rat tail collagen type I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 8 ug/mL Hu FGF-b (Life Technologies cat. # PHG0263) and 2 mg/mL Heparin (Sigma-Aldrich H3149 ...
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bioRxiv - Cell Biology 2024Quote: ... selected using 8 µg/mL of blasticidin (Thermofisher, A11139-03), transduced with the pLVTRE3G-construct of interest ...
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bioRxiv - Developmental Biology 2024Quote: ... differentiation was started by replacing with Essential 8 medium (Gibco) containing BMP4 (80 ng/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was changed to Essential 8™ Medium (Gibco). Individual iPSC colonies were mechanically picked and expanded as clonal lines on Geltrex-coated cell culture dishes in Essential 8™ Medium ...
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bioRxiv - Bioengineering 2024Quote: ... 8-well chambered coverglasses (Nunc Lab-Tek II, Thermo Scientific) were coated with rat tail collagen type I (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... for overnight at 65°C and RNA was purified by acid-phenol:chloroform pH 4.5 (Thermo Fisher Scientific). The details for PKR fCLIP are included in Supplementary Appendix II.
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bioRxiv - Biochemistry 2022Quote: ... and after immobilization of HER2- Nb and HER2-Nb-GEPII 1.0 was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) reagent (ThermoFisher). After immobilization of HER2-Nb and HER2-Nb-GEPII 1.0 on the cell surface ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with 1.2mM 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Cancer Biology 2024Quote: Proliferation assays were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific) assay ...
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bioRxiv - Genetics 2020Quote: ... him-8(tm611) germlines were immunostained with 1:500 rabbit anti-GFP (Thermo Fisher A-11122), and all other germlines were stained with 1:2000 mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in PBS containing 8 μg ml-1 Alexa Fluor 647 NHS ester (Invitrogen) and incubated at room temperature for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... and plated onto Matrigel-coated wells in Essential 8 medium containing Revitacell (1:200 dilution; Gibco) at 3.9 × 104/cm2 in 12-well cell culture plates ...
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bioRxiv - Cell Biology 2022Quote: ... the hiPSCs were resuspended in 1 mL of Essential 8 Flex Basal Medium (Thermo Fisher Scientific) and were plated in agarose inserts at two different cell densities ...
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bioRxiv - Cancer Biology 2020Quote: ... except C3H/10T 1/2 Clone 8 was cultured in Eagle’s Basal medium (Thermofisher, #21010-046) supplemented with HI-FBS to a final concentration of 10% and 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... THP-1 cells were cultured in RPMI supplemented with 8% FBS and antibiotics (penicillin, streptomycin, GIBCO) at 37°C with 10% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2023Quote: ... HCT-8 cells (ATCC CCL-244) and THP-1 cells were maintained in RPMI-1640 (Invitrogen), supplemented with 10% HyClone Cosmic calf serum (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of purified bacmid plasmid DNA was mixed with 8 μL of Cellfectin II (ThermoFisher) at room temperature to transfect 0.9×106 Sf9 cells per well in a 6-well tissue culture plate in a semi-humid environment ...
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bioRxiv - Molecular Biology 2023Quote: 1 x 107 cells at the exponential growth phase were labeled with 8 µM CFSE (Invitrogen) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Developmental Biology 2019Quote: ... One-third to one embryo equivalents were loaded at per lane on 4%-12% or 8% Bolt® Bis-Tris (Invitrogen). GFP Rabbit IgG Polyclonal Antibody (Molecular Probes ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was cleared by centrifugation at 12,000 × g at 4 °C for 40 min in a Fiberlite TM F15-8 × 50 cy Fixed Angle Rotor (ThermoFisher Scientific). The resulting lysate was filtered through a 0.45 μm syringe filter and loaded onto a 5 ml HiTrap™ TALON Crude cassette (GE ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
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bioRxiv - Cell Biology 2020Quote: ... 14 mM Glucose and 4 mM L-AlanylGlutamine supplemented with 8% heat-inactivated fetal bovine serum (FBS) (#10082139; Thermo Fisher Scientific), EGF 5 ng/ml (#01-107 Millipore ...
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bioRxiv - Microbiology 2022Quote: ... mutations were inserted in fragment 8 (Supplementary Table 4) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, Cat# A13312) according to the manufacturer’s protocol with the primers listed in Supplementary Table 5 ...
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bioRxiv - Systems Biology 2022Quote: Cells were cultured and transfected (as described above) for CLSM experiments on 4 or 8-well Lab-Tek™ chambered cover glass slides (ThermoFisher). Before imaging ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were analyzed by using an 8 capillary ABI 3500 DNA Genetic Analyzer with POP-4 polymer (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Immunology 2020Quote: 7575 naïve CD4 cells were isolated from lymph nodes of CCT8T+/+ and CCT8T−/− mice at 4-8 weeks of age by FACS sorting directly into lysis buffer (RIPA buffer (ThermoFisher Scientific) + 4 % IGEPAL CA-360 (Merck) ...
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bioRxiv - Immunology 2022Quote: ... Cell debris was removed from lysate containing soluble protein by centrifugation at 35,000 x g and 4 °C for 45 min (Sorvall LYNX 6000 centrifuge, F21-8×50y rotor, Thermo Fisher Scientific). Proteins were further purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were cultured to confluence and passaged 4 times before plating to 8-chamber slides (Thermo Fisher Scientific Cat#177445) coated with Matrigel (Thermo Fisher Scientific Cat# CB-40230 or Corning Cat# 356230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated twice for 30 s and then cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Amylose beads (Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...