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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... and 1×2-Mercaptoethanol (GIBCO™). The base medium was supplemented with 3.3mM CHIR-99021 (Selleckchem) ...
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bioRxiv - Genetics 2024Quote: ... N-2 (1:100; Thermofisher, 17502048), B27 without vitamin A (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% N-2 (Thermo Scientific, 17502048), 0.1% 5-Fluoro-2′-deoxyuridine (FRDU ...
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bioRxiv - Evolutionary Biology 2024Quote: ... then incubated at 37C for 5 min in 2 ml M3+BPYE media containing 1 ml Turbo DNase (2 U/μL) (ThermoFisher cat. no. AM2239) per 36 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were incubated at 4□ for 30 min with a PE-conjugated anti-Tra-1-60 antibody (Thermo Fisher Scientific, 12-8863-82, 1:5) or a PE-conjugated anti-SUSD2 antibody (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant (1 ml at ~4-5 mg/ml) was incubated with 50 μl of superparamagnetic Dynabeads Streptavidin C1 (Invitrogen) and rotated overnight at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at 37°C (with 5% CO2 and humidity) before indicator loading with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Genomics 2024Quote: ... using 5 μl/well (6-well plate) or 1 μl/well (24-well plate) of Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Genetics 2022Quote: ... fixed ovaries and eggs were incubated in a 300 nM DAPI stain solution (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) on a shaking platform (150-200 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was placed on slides and cover-slipped with mounting media containing DAPI (Fluoromount-G; 4’,6-diamidino-2-phenylindole; Invitrogen). All immunofluorescence runs contained tissue from more than one age (1 ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were counter stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in ProLongTM Gold antifade mounting media (Invitrogen). Images were captured using Nikon C2 confocal microscope.
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were washed 3 × 5 minutes in PBS and finally mounted onto glass slides using Prolong Gold mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher). Images were acquired via confocal microscopy using a Zeiss LSM-880 confocal microscope (Plan-Apochromat 40x/1.3 Oil DIC UV-IR M27objective ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific) and allowed to set overnight ...
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bioRxiv - Genetics 2020Quote: ... then incubated for 2h at room temperature with Alexa Fluor secondary antibodies (S2 Table) and nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Images were captured on a BX51 fluorescence microscope (Olympus ...
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bioRxiv - Molecular Biology 2022Quote: ... the sections were mounted with ProLong Gold with 4′,6-diamidino-2-phenylindole (DAPI) (catalog no. P36935, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: The membrane probes Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were obtained from Molecular Probes (Eugene, OR, USA) and dissolved in ethanol and dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 3×106 MART-1-transduced T cells were added and incubated for the indicated hours (2, 4 or 6) in “Intermediate” 4K6R medium consisting of RPMI 1640 Medium for SILAC (88365, Thermofisher) with 10% dialyzed fetal bovine serum (15605639 ...
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in a flow-cytometry buffer and DAPI (4′,6-diamidino-2-phenylindole, 0.5 μg/ml; ThermoFisher #D1306) solution to exclude dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed in 4% formaldehyde at room temperature for 10 minutes and mounted onto slides using ProLong Gold or Diamond Antifade Mountant with 4′,6-diamidino-2- phenylindole (DAPI) (ThermoFisher). Images were obtained using a Leica SP8 confocal microscope using excitation/emission spectra ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampal sections were stained with 4’,6-diamidino-2-phenylindole (DAPI) and cresyl violet or fluorescent Nissl (NeuroTrace 435/455 Blue, ThermoFisher). OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP ...
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bioRxiv - Genetics 2023Quote: ... ISE6 cells were fixed with 4% paraformaldehyde followed by 1 x PBS washes and the coverslip was mounted on a slide using Antifade gold mounting reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) and observed under the Nikon W-1 Spinning Disk confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... a working solution of 0.83 mg/mL solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, 22589; Thermo Fisher) in PBS was prepared from a stock solution of 83 mg/mL sulfo-SANPAH in DMSO (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI) or anti-phalloidin for F-actin (Invitrogen) at 25 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Hoechst 43222 (H1399) and ProLong Gold Antifade Mountant with 4′,6- diamidino-2-phenylindole (DAPI; P36931) were purchased from Invitrogen.
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bioRxiv - Cell Biology 2022Quote: ... The plates were then washed with PBS 3×10min at RT on a shaking plate and cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...