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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Ligand overlay assays were performed on PVDF-FL membranes using mouse Engelbreth– Holm–Swarm (EHS) laminin (ThermoFisher, 23017015). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... the protein concentration was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce). Volumes corresponding to 30 µg of protein were separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2022Quote: ... EdU-labeling assays were performed using the Click-iT Plus EdU AlexaFluor488 cytometry assay kit (Life Technologies, Inc.), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was removed and a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227) was used to calculate protein concentration.
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bioRxiv - Cancer Biology 2020Quote: ... Then exosomal protein was quantified by Bradford assay using Pierce™ BCA Protein Assay Kit (Thermo Scientific™) and serial dilution of BSA standard (Thermo Scientific™).
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bioRxiv - Cancer Biology 2020Quote: ... Pierce™ bicinchoninic acid (BCA) protein assay kit and radioimmunoprecipitation assay (RIPA) buffer were sourced from Thermo Fisher Scientific Inc ...
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bioRxiv - Immunology 2022Quote: ... and the concentration determined by a BCA assay using the Pierce BCA Protein assay kit (Thermo Fisher Scientific). Protein samples were aliquoted and stored at -80°C for future use.
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were collected and total protein was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific) using a FLUOstar Omega Microplate Reader (BMG LABTECH) ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was taken to protein quantification by bicinchoninic acid assay (Pierce BCA Protein Assay Kit, Thermo Fisher). Equal amounts of protein were taken to NuPAGE LDS Sample Buffer (4X ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
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bioRxiv - Biochemistry 2023Quote: ... a differential scanning fluorimetry assay based on the use of SYPRO orange (Thermoshift assay kit, Thermo Fisher Scientific) was used to minimize protein consumption (Huynh & Partch ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and the concentration determined using a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). After running an SDS PAGE (12%) ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting protein concentrations were determined with a Bicinchonic acid assay (BCA - Pierce BCA Protein Assay Kit, ThermoFisher, Germany) and 20ug of protein were filled up to 140ul with 100mM HEPES pH 8 and transferred to a 96 deep-well plate ...
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bioRxiv - Biochemistry 2024Quote: The in vitro phospholipase activity assay was performed using the Amplex Red Phospholipase D Assay Kit (A12219, Invitrogen) and 1,2-dioctanoyl-sn-glycero-3-phosphocholine (8:0 PC ...
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bioRxiv - Developmental Biology 2023Quote: ... The total protein yield was determined by Pierce Bradford assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific). Proteins were then transferred to membranes and immunoblotting was performed using the antibodies described in Supplementary Table 2.
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bioRxiv - Plant Biology 2023Quote: ... Protein concentrations were measured using the Bradford assay (Pierce™ Detergent Compatible Bradford Assay Kit, Thermo Scientific, USA), and equal amount of proteins were loaded on a SDS-PAGE gel for western blot.
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bioRxiv - Cancer Biology 2024Quote: ... Protein measurement was determined using Pierce™ BCA Protein Assay Kits BCA assay (Thermo Scientific™, Cat# 23225) using Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set (Thermo Scientific™ ...
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bioRxiv - Microbiology 2023Quote: ... a modified bicinchoninic acid assay (BCA) protocol was used (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific) [62] ...
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bioRxiv - Microbiology 2022Quote: ... After quantification of amplicons via a picogreen assay (Quant-iT™ PicoGreen™ dsDNA Assay Kit, Thermo Fisher), 2 ng of each product were pooled for subsequent cleanup using the AmpureXP PCR cleanup protocol (Beckman) ...
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bioRxiv - Physiology 2023Quote: ... supernatant protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Cat#23225, Thermo Scientific). Twenty μg/lane was run (200V ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was carried out using Click-IT Plus TUNEL assay kit (Molecular Probes, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was carried out using Click-IT Plus TUNEL assay kit (Molecular Probes, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: The cell proliferation assay was performed using the Click-iT® EdU Flow Cytometry Assay Kits (C10424, Invitrogen) following the vendor’s protocol with some modifications ...
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bioRxiv - Bioengineering 2024Quote: ... The protein concentration of each sample was determined by BCA assay (Thermo Scientific, Pierce BCA Protein Assay Kit) relative to a bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: Protein concentration was quantified using the BCA assay (Pierce™ BCA Protein Assay Kits, 23225, Thermo Scientific™). For this ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were calculated with BCA assay according to manufacturer’s recommendation (Pierce BCA Protein Assay Kit, Thermo Fisher) and 15 µg protein extracts were diluted with Milli-Q® water and mixed with 6x Laemmli buffer to a final concentration of 1x ...
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bioRxiv - Cancer Biology 2024Quote: ... and BCA assay was performed to measure protein concentration using Pierce BCA Protein Assay Kit (Thermo Fisher, UK).
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bioRxiv - Cancer Biology 2024Quote: Cell cycle assay was performed using Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were visualized by an enhanced chemiluminescent kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were visualized by ECL kits (#NEL105001EA, PerkinElmer; #34580, Thermo Scientific).
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Membranes were visualized using a LiCOR or Femto HRP kit (Thermofisher 34094). Uncropped images of Western blots are provided as Supplemental Figure 1.
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bioRxiv - Biochemistry 2021Quote: ... membranes were developed using enhanced chemiluminescence system (ECL-Kit, Thermo Scientific, Germany) and X-ray film (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The potential-sensitive dye FluoVolt™ Membrane Potential Kit (Thermofisher, USA, F10488) was used for the action potential recordings according to protocol described in (Slotvitsky et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were visualized using ECL Western Blotting Detection Kit (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Membranes were developed with Metal Enhanced DAB Substrate Kit (Thermo Fisher Scientific). Blot images were taken with Genesys acquisition system (Syngene ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were developed with the Pierce ECL Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Bioengineering 2021Quote: ... 16 Protein concentration of the extracts was estimated using a BCA protein assay kit (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, Oakville, ON, Canada) as per supplier’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Genomics 2021Quote: DNA concentration was measured with the Qubit ssDNA Assay Kit and Qubit dsDNA HS Assay kit on a Qubit Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The purified DNA was stored at −20 °C until use.
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bioRxiv - Biochemistry 2021Quote: ... and quantified using a commercially available BCA kit (Pierce™ BCA Protein Assay Kit, ThermoFisher) according to the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... and quantified using a fluorometric kit (Quant-iT PicoGreen dsDNA Assay Kit; Invitrogen, Cat#P7589). Purified amplicons were pooled in equimolar concentrations with a SequalPrep Normalization Plate Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... DNA was quantified using the Quant-iT dsDNA Assay kit high sensitivity kit (ThermoFisher, USA). Microbial community composition was determined by Illumina sequencing of the V4 region of the 16S rRNA gene followed by analysis using QIIME 2 2019.7 (Bolyen et al. ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). The products were analysed on a 7500 Fast Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... [35S]-Methionine-labeled protein samples were solubilized in SDS sample buffer containing 20 mM dithiothreitol and electrophoresed in parallel with molecular mass markers (SeeBlue® Plus2 Pre-Stained Standard, Invitrogen) on 8% Tricine-SDS polyacrylamide gels ...
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bioRxiv - Neuroscience 2022Quote: ... matched sections were labelled in parallel using goat anti-parvalbumin (PV) (1:1000, Swant) followed by donkey anti-goat Alexa Fluor 488 (1:1000, Thermo Fisher). For somatostatin interneuron counts (In Sst-Cre/+ ...
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bioRxiv - Genomics 2021Quote: ... plasmid DNA from each library design was amplified in ten parallel reactions (10 ng DNA per reaction) using Phusion DNA Polymerase (Thermo Fisher) as above ...
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bioRxiv - Neuroscience 2022Quote: ... matched sections were labelled in parallel with rabbit anti-μOR (1:1000, immunoStar) followed by donkey anti-rabbit Alexa Fluor 647 (1:500, Thermo Fisher). Image data were acquired using a 20X objective and z-stack tile approach on a Zeiss LSM700 confocal microscope ...