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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Cryopreserved hiPSCs were thawed and plated into 6-well culture plates coated with 1.1 µg/cm2 recombinant human laminin-521 (rhLN-521, A29248, Gibco) in 2 ml mTeSR Plus medium (100-0274 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were seeded onto 96-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (rhVTN-N; ThermoFisher #A31804). Cells were allowed to adhere for >3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated with a primary antibody for PHH3 (Ser10) Recombinant Rabbit Monoclonal Antibody (9H12L10) (Cat# 701258, Invitrogen) at 1:5000 dilution in PBS and BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were treated with a Kv1.1 recombinant rabbit monoclonal antibody (SN66-06, ThermoFisher Scientific) overnight and with an Alexa-488 conjugated secondary antibody for two hours on the following day ...
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bioRxiv - Developmental Biology 2024Quote: ... or Alexa Fluor 488– or Alexa Fluor 568–conjugated alpaca recombinant secondary antibody (Invitrogen) or Alexa Fluor 647–conjugated wheat germ agglutinin (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... T8ML-1 cells derived from a patient with refractory peripheral T cell lymphoma were unstimulated or stimulated with anti-CD3/28 Dynabeads (Invitrogen) in the presence of soquelitinib or DMSO for one hour ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Pathology 2024Quote: ... Primary antibody used was 1:400 rabbit anti-human IBA1 (Wako 019-19741, Richmond, VA) with secondary antibody 1:450 anti-rabbit Alexa Fluor 488 (Invitrogen A21206, Waltham, MA). Immunofluorescence was analyzed and images acquired using a Zeiss LSM780 laser scanning confocal microscope ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse CD19+ or anti-mouse B220+ (Thermo Fisher, clone RA3-6B2), anti-mouse GL-7+ (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... recombinant (Invitrogen, # 10342020) and 100 mM dATPs ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... All stains were carried out in media containing anti-CD16/32 blocking antibody (ThermoFisher, clone 93, catalog # 14-0161-86). All flow cytometry was acquired on an LSRFortessa FACS analyzer ...
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bioRxiv - Cancer Biology 2021Quote: ... The cleared supernatants were incubated with 50 µl of beads pre-coated with 40 µg of anti-PTBP1 antibodies (clone BB7) or mouse IgG Isotype Control (Invitrogen). Immunoprecipitations were conducted overnight at 4°C with constant shaking ...
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bioRxiv - Immunology 2021Quote: ... Sixteen hours later 0.1 x106 HUCCT1 cells were added to each well in the presence of increasing concentrations of anti-EGFR antibody (mouse IgG2a, clone 528, Invitrogen). Three days later ...
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bioRxiv - Bioengineering 2022Quote: ... at 4°C in PBS containing 0.3% Triton X-100 and 0.5% bovine serum-albumin with the following primary antibodies: mouse IgM anti-Stro-1 (Clone STRO1, Thermo scientific). Detection was achieved using an anti-mouse IgM-Alexa 555 antibody for one hour at +4°C ...
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bioRxiv - Pathology 2020Quote: ... Department of Comparative Medicine on formalin-fixed paraffin embedded sections using the following primary antibodies: rat monoclonal anti F4/80 (Clone BM8, Invitrogen, Cat ...
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bioRxiv - Immunology 2024Quote: ... staining was performed in the presence of 50 nM dasatinib at a 1:75 dilution and anti-CD8 antibody clone KT15 (ThermoFisher) was used to minimize background signal ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Immunology 2023Quote: ... or stained with 5µg/ml anti-CD284 (TLR4) antibody (clone UT41) conjugated with Alexa Fluor 488 (Invitrogen, #53-9041-82) or identical amount of mouse IgG1 kappa isotype control (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C: IL-15Rα-PE (clone DNT15Ra; Invitrogen), CD40-BV421 (clone 3/23 ...
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bioRxiv - Cell Biology 2022Quote: ... 200μl of staining solution containing flow cytometry antibodies (anti-CD45-PECy7 (1:100, clone 30-F11, Invitrogen, 25-0451-82), anti-CD64-PE/Dazzle594 (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in 5% blocking serum at room temperature before being incubated overnight in the following primary antibody solutions (TBST, 1% serum): anti-CD4 (clone RM4-5, ThermoFisher), anti-CD8 (clone 4SM15 ...
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bioRxiv - Immunology 2024Quote: ... mice were intravenously injected with 3 µg of anti-CD45.1-PE antibody (Thermo Fisher Scientific, 12-0453-82, clone: A20) in 200 µL of PBS three minutes prior to euthanization to preferentially label blood-derived lymphocytes (Ruscher and Hogquist ...
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bioRxiv - Immunology 2024Quote: ... 250 μL of medium containing the blocking antibody was added to the cultures to a final concentration of 25 μg/mL anti-CD1d (clone 1B1, Invitrogen), 20 μg/mL anti-NKG2A/C/E (clone 20d5 ...
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bioRxiv - Immunology 2024Quote: ... were incubated overnight at 4°C with 500 µL/well of sterile ddH2O supplemented with 1 μg/mL anti-CD3 monoclonal antibody (mAb) (clone OKT3; Invitrogen) and 1 μg/mL anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Microbiology 2024Quote: ... and then stained for intracellular cytokines with the following antibodies: anti-IFNγ (Clone: XMG1.2; Fluorophore: BV421; Fisher Scientific; cat# BDB563376) and anti-IL17a (Clone ...
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bioRxiv - Cancer Biology 2020Quote: ... and SK-MEL-28 cell lines were cultured in DMEM (Gibco) with 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified Trophoblasts were seeded in DMEM-HAM’s F12 (Gibco 31331-28)/ 10% FBS (Sigma S0615-500ML ...
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bioRxiv - Genetics 2022Quote: ... 28/18S were assessed by NanoDrop ND-2000 spectrophotometer (Thermo Scientific). Over five milligrams of RNA were obtained per cell line ...
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bioRxiv - Genetics 2024Quote: ... Cells were incubated at 28°C humidified incubator (Binder, Thermo Scientific) with normal air composition ...
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bioRxiv - Microbiology 2023Quote: ... and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Splenocytes from CD2rtTA x DN-IKK2 mice were stimulated in vitro at 1×106 cells/ml with 10 mg/ml anti-CD3 (clone 145-2C11) and 10 mg/ml anti-CD28 (clone 37.51) (ThermoFisher Scientific, Waltham, MA). Following 24 hours of stimulation ...
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bioRxiv - Immunology 2020Quote: ... functional grade anti-mouse IL-4 (eBioscience™, clone: 11B11), functional grade anti-mouse INF-γ (eBioscience™, clone: XMG1.2) were from ThermoFisher, Loughborough ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human Alexa-647 [Cat# A21445] and streptavidin-AlexaFluor 635 [Cat# SA1011] antibodies were obtained from Thermo Fisher. Control lectins Erythrina cristagalli agglutinin (ECA ...
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used at 1:200 for IF experiments: Anti-human-Alexa-633 (Life Technologies #A21091), anti-mouse-Alexa-488 (Life Technologies #A11029) ...
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bioRxiv - Microbiology 2020Quote: ... Meso Scale Discovery (MSD)) were coated with mouse anti-human IgG antibody (CH2 domain, cat# MA5-16929, ThermoFisher Scientific) at 2 μg/mL in 1X PBS (50μL/well) ...
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bioRxiv - Cell Biology 2021Quote: ... The slides were washed and incubated for 2 h with secondary antibodies (Alexa 488 anti-Human IgG Thermo Fisher - #A11013 and Alexa Fluor 555 Anti-Mouse IgG #A21422) ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Immunology 2022Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of alkaline phosphatase-conjugated goat anti-human F(ab’)2 antibody (Thermo Fisher Scientific) Inc. ...