Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 4850 citations for CD40L Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: KOLF2 human iPSCs were maintained in E8 flex media (ThermoFisher) and manually dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies ...
-
bioRxiv - Immunology 2024Quote: ... and stimulated with human CD3/CD28 Dynabeads (Life Technologies, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and OxPhos Human WB Antibody Cocktail (Thermo Fisher 45-8199). Detection of protein signals was performed using ECL Prime Western Blotting Detection Reagent (Cytiva RPN2236 ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK 293FT human embryonic kidney cells (Thermo Fisher Scientific, #R70007) were cultured in DMEM (Corning ...
-
bioRxiv - Physiology 2024Quote: ... Human neutrophils were cultured in RPMI-1640 media (Gibco, #52400017) + 10% FCS + 1% penicillin/streptomycin and the purity of isolated neutrophils was confirmed as >98% CD45+ CD15+ via flow cytometry (anti-CD45-FITC ...
-
bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
-
bioRxiv - Molecular Biology 2024Quote: ... and human HEK293T cells was extracted using TRIzol reagent (Invitrogen). Endogenous RNase was further removed by ethanol precipitation ...
-
bioRxiv - Cancer Biology 2023Quote: ... LAN5 human neuroblastoma cells were cultured in RPMI (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... human H2BC1 and H2AZ2 cDNA were synthesized by Thermo Fisher and cloned into the pCW-Cas9 vector (Addgene #50661 ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Human CD11b-APC conjugate ( Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
-
Intranasal Delivery of shRNA to Knockdown the 5HT-2A receptor Enhances Memory and Alleviates AnxietybioRxiv - Neuroscience 2023Quote: ... we chose the human specific 5HT-2A antibody from ThermoFisher PA5-120747 at 1:50 with secondary Goat Anti-Rabbit Fluor555 at 1:1000 using a MOI of 3 ×105 ...
-
bioRxiv - Neuroscience 2023Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used to quantify soluble IL-6Ra expression in cell culture media ...
-
bioRxiv - Immunology 2024Quote: ... anti-human CD45 (clone: MEM-28) conjugated to AF488 (ThermoFisher) was used ...
-
bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney 293T (ATCC) were grown in (Invitrogen) supplemented with 10% fetal bovine serum (Gibco) ...
-
bioRxiv - Immunology 2023Quote: ... Stimulation with αCD3/CD28 DynaBeads Human T cell activator (Invitrogen) (cell to bead ratio of 1:1 ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by anti-human IgG–Alexa594 (1:1000, ThermoFisher Scientific) for 30 min and analyzed by flow cytometry ...
-
bioRxiv - Biophysics 2023Quote: ... were cultured in human endothelial serum-free medium (SFM, Gibco) containing 20% foetal bovine serum ...
-
bioRxiv - Molecular Biology 2024Quote: Human neonatal epidermal melanocytes (HEMn-DP, Thermo Fisher Scientific, C2025C) were maintained in Medium 254 (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
-
bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
-
bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
-
bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
-
bioRxiv - Biophysics 2021Quote: ... Human embryonic kidney 293 cells (HEK293) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... anti-human CD14 (11-0149-42, Thermo Fisher Scientific, 1:100), anti-human CD117 known also as cKIT (12-1178-42 ...
-
bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
-
bioRxiv - Microbiology 2021Quote: ... or a type IV DQTM collagen conjugate from human placenta (Invitrogen). Reactions were prepared in technical triplicate or quadruplicate by mixing 20 µL of substrate at 0.25 mg/mL with 80 µL of reaction buffer and 100 µL of bacterial suspension ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary human mammary epithelial cells (HuMECs) were provided by Thermo Fisher Scientific and cultured according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: Cultured primary human airway epithelial cells were washed with PBS (Gibco), fixed with 4% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Cell Biology 2020Quote: ... Microarray analysis was performed with the Human Gene 1.0ST Array (Affymetrix). Protein expression was analyzed by Simple Western (ProteinSimple) ...
-
bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific, MA, USA), FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific ...
-
bioRxiv - Genetics 2021Quote: ... We quantified expression using the IgG4 Human ELISA kit (Invitrogen BMS2095) with IgG4 standards provided in the kit ...
-
bioRxiv - Genetics 2021Quote: Human TERT cDNA39 was cloned into the gateway pDONR221 plasmid (Invitrogen). TERT variants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... human testicular cells were suspended in MEMα medium (Thermo Fisher Scientific) containing 10% FBS (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Human ESCs were cryopreserved in PSC Cryomedium (Gibco, Cat. No. A2644601). The culturing medium was supplemented with 1X RevitaCell (100X ...
-
bioRxiv - Cell Biology 2022Quote: ... Human NGFR siRNA or negative control siRNA were obtained from ThermoFisher Scientific (Waltham ...
-
bioRxiv - Neuroscience 2021Quote: ... human neurons were transfected with Lipofectamine 3000 (#L3000015, Thermo Fisher Scientific) and 1.6 μg of plasmid DNA at 40 - 50 DIV ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary human and NHP microglia were cultured in DMEM/F12 (Gibco) supplemented with 10% defined fetal bovine serum (HyClone) ...
-
bioRxiv - Genomics 2020Quote: ... 20x human TERT TaqMan™ Copy Number Reference Assay (ThermoFisher 4403316) as internal reference control ...