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Citations for Thermo Fisher :
951 - 1000 of 1081 citations for p p' DDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The plate was washed 5 times before being developed with phosphatase substrate PNPP (p-nitrophenyl phosphate) (Thermo Scientific, product number 34047) for 20 min and read at OD405 on a Biotek H1 synergy plate reader.
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bioRxiv - Synthetic Biology 2022Quote: ... proteins were transferred from gels onto 0.45-μm Immobilon-P polyvinylidene difluoride membranes (PVDF) using a mini blot module (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS; SAFC Biosciences) and 1% (v/v) Penicillin/Streptomycin (P/S; Gibco, Life Technologies). Primary fibroblasts were maintained in F-DMEM culture medium comprising DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... #1 Coverslips (Fisherbrand™, #12-545-MP) were mounted using ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific, #P-36931). We used rabbit anti-CUX1 (1:200 ...
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bioRxiv - Pathology 2022Quote: We extracted total RNA of tissues from MI sites for MI and MI treated by P groups using TRIzol (Invitrogen, USA). In the Sham group ...
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bioRxiv - Bioengineering 2023Quote: ... all reservoirs for the pressure sources (P-CAP, Fluigent) were filled with 1X phosphate-buffered saline (PBS) (Gibco PBS, Fisher Scientific). All pressure measurements were made using Honeywell pressure sensors (ABPDRRV015PDAA5 ...
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bioRxiv - Bioengineering 2022Quote: ... qPCR-based copy number assay was carried out using Taqman Copy Number Assay and RNAse P Reference Assay (Thermo Fisher Scientific). qPCR probes specific to the GFP reporter gene was custom ordered from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and GlyA-CHO cells (glycine auxotrophic for growth and a gift from Dr. P. J. Stover, Cornell University) using Lipofectamine 2000 (Invitrogen # 11668019). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 mg/ml fibrinogen and 0.2% LAP for photoinitiation and cultivated in vitro for up to 5 days with endothelial basal medium containing 0.1% FBS and 1% P/S (Thermo Fisher Scientific). For rescue experiment ...
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bioRxiv - Microbiology 2023Quote: ... or GFP targeting siRNA as a negative control (cat no. P-002048-01-20, Dharmacon) using Lipofectamine 3000 transfection reagent (cat no. L3000-008-Invitrogen/ Thermofisher scientific). Total cell extract was collected 24hrs after the second siRNA transfection to measure the knockdown efficiency by western blotting.
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bioRxiv - Neuroscience 2023Quote: ... it was gently pipetted into 10 mL of pre warmed complete RPMI medium consisting of basal RPMI-1640 supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (P/S; ThermoFisher Scientific). Following centrifugation (300xg for 5 min at room temperature (RT)) ...
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bioRxiv - Bioengineering 2023Quote: ... Activity of purified uricases and p(Man)-uricase constructs were measured using AmplexTM Red Uric Acid/Uricase Assay kit (Thermo Fisher). Injections were administered once per week intravenously in the tail vein.
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bioRxiv - Genetics 2023Quote: ... total RNA was extracted from stage 14 oocytes expressing GL00409 with P{matalpha4-GAL-VP16}V37 using the TRIzol Reagent (Life Technologies). cDNA was made using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: The δ2H of BPs was analyzed on a GC-pyrolysis-isotope ratio MS (GC-P-IRMS) on a GC IsoLink II IRMS System (Thermo Scientific) in the Organic Geochemistry Laboratory at the University of Colorado Boulder ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then probed with anti-p75NTR (1:200; rabbit monoclonal, kindly gifted by P. Barker) and anti-GAPDH 1:8000 (mouse monoclonal IgG; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... All plasmids were purified with AxyPrep Plasmid Purification Miniprep Kit (Axygen, #AP-MN-P-250) and PureLink Quick PCR purification Kit (Invitrogen, #K310001). mMESSAGE mMACHINE® T7 Ultra Kit (Life Tech ...
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bioRxiv - Immunology 2024Quote: ... and the supernatant was collected and subjected a serial 10-fold dilution in DMEM supplemented with 1% penicillin-streptomycin (DMEM + P/S) (Thermo Fisher). When MDCK cells reached 90% confluency in each well ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was carried out in antibiotic-free culturing media (6ml) using 10μl lipofectamine (Lipofectamine ® 2000Reagent, 1mg/ml, Invitrogen P/N 2887) in 400μl Opti-mem media (Gibco Opti-mem reduced serum media ...
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bioRxiv - Microbiology 2019Quote: ... and cultured in Minimum Essential Medium Alpha Modification (MEM-α, HyClone) with 20% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (P/S, Gibco Life Technologies). Goat adipose-derived mesenchymal stem cells (gASCs ...
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bioRxiv - Genomics 2020Quote: ... or No Call (numerical representations 0, 1, 2, −1, respectively) using the BRLMM-P algorithm for 493,290 SNPs [25] (Affymetrix Power Tools (APT) Release 1.16.0) ...
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bioRxiv - Neuroscience 2022Quote: ... it was gently pipetted into 10 ml of pre warmed complete RPMI medium (CM) consisting of basal RPMl-1640 supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (P/S; Thermo Fisher Scientific). Following centrifugation (300 g for 5 min at room temperature) ...
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bioRxiv - Developmental Biology 2022Quote: ... GEMs were broken and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific; P/N 37002D). cDNA was amplified using the C1000 Touch Thermal cycler with 96-DeepWell Reaction Module ...
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bioRxiv - Molecular Biology 2021Quote: ... liquid chromatography was performed with nanoUHPLC Easy nLC 1000 (Thermo Scientific brand, model EASY-nLC 1000, Easy-Spray ColumnPepMap RSLC, P/N ES801) (Thermo Scientific) coupled to a mass spectrometer with Orbitrap technology ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μl supernatant and 100 μl diluted cell lysates were independently incubated with 100 μl AP substrate p-nitrophenyl phosphate (PNPP) (Thermo Scientific, 37620) at room temperature and the absorbance was measured at 405 nm by a plate reader (SpectraMax M3 ...
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bioRxiv - Biophysics 2022Quote: ... supplemented with MSC Growth Kit Low Serum (ATCC, PCS-500-040, VA, USA) and 0.1% v/v penicillin-streptomycin (P/S, Invitrogen, 15140, Barcelona, Spain). Medium was replaced every 2-3 days ...
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bioRxiv - Biophysics 2022Quote: ... passage p<=6) were obtained from the Radboud Biobank and cultured in Minimum Essential Medium Eagle (α-MEM) (Invitrogen, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
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bioRxiv - Microbiology 2020Quote: ... at a flow rate of 0.3 μL/min and separated on a C18 analytical column (75 um x 50 cm; PepMap RSLC C18; P/N ES803; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The barcode sequencing libraries were quantified using a Qubit 2.0 with a Qubit™ dsDNA HS Assay Kit (Invitrogen P/N Q32854) and the quality of the libraries were performed on a 2100 Bioanalyzer from Agilent using an Agilent High Sensitivity DNA kit (Agilent P/N 5067-4626) ...
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bioRxiv - Neuroscience 2020Quote: ... sequences were amplified from plasmids derived from ser-2(2)p::FRT::YFP,andclonedupstreamof FRT::STOP::FRT::GCaMP6s to generate WY016 using Gateway (Thermo Fisher Scientific). To generate PSAB1206 ...
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bioRxiv - Cell Biology 2022Quote: ... in islet culture media (RPMI media with 11.1 mM D-glucose supplemented with 10% vol/vol fetal bovine serum (FBS) (Thermo: 12483020) and 1% vol/vol Penicillin-Streptomycin (P/S) (GIBCO: 15140-148)) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... was used according to the manufacturer’s instructions in conjunction with plasmids pASK-GFP-mKate2-L2 (kind gift of Dr. P. Scott Hefty) cut with FastDigest AgeI and EagI (Thermofisher, Waltham, MA), pBOMB4-Tet (kind gift of Dr ...
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Bioengineering 2019Quote: ... Tissues were cultured in iCell plating media (iCell CMM-100-010-001) with 1X penicillin/streptomycin/Amphotericin B (P/S/A, Gibco cat# 15240062). After 48h ...
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bioRxiv - Systems Biology 2019Quote: ... fold-change ratios and p-values for the proteomics data were calculated by Proteome Discover™ 2.2 (Thermo Scientific™, Waltham, MA). FPKM values ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant bacmid containing hPIV3 L-P genes was generated and isolated following the instruction manual of the bac-to-bac baculovirus expression system (Thermo Fisher Scientific). Viral stocks generated from purified bacmids were amplified and used for protein expression ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
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bioRxiv - Bioengineering 2021Quote: ... The primate ovaries were dissected into approximately 60 fragments measuring 1×1×1mm (1 mm3) and transferred into maintenance media (α- MEM, P/N 32561, Gibco, USA) prior to encapsulation (Figure 4) ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Systems Biology 2021Quote: ... The UHPLC was equipped with a trap column (Acclaim PepMap 100, 75 μm x 2 cm, C18, P/N 164535, Thermo Fisher Scientific) and a 50 cm analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 μg/ml streptomycin (P/S, Mediatech, Manassas, VA) and buffered to pH 7.35 with 26 mM NaHCO3 (Fisher Scientific, Pittsburgh, PA). HEK 293 cells transfected with either wild-type TRPV4 (WT) ...
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bioRxiv - Microbiology 2021Quote: ... The gene of VSV P protein were amplified and synthesized in vitro using mMESSAGE mMACHINE® Kit (Life technologies, Cat. No. AM1344) to serve as a standard ...
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bioRxiv - Microbiology 2020Quote: ... was used according to the manufacturer’s instructions in conjunction with plasmids pASK-GFP-mKate2-L2 (kind gift of Dr. P. Scott Hefty, Univ. Kansas) cut with FastDigest AgeI and EagI (Thermofisher, Waltham, MA), pBOMB4-Tet (kind gift of Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended at a concentration of 1×104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1×103 cells ...
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bioRxiv - Cell Biology 2022Quote: ... To prepare protein lysate the cells were scrapped off using cell scrapper and lysed in RIPA buffer (50 mM Tris pH-7.4, 150 mM NaCl 1% Nonidet P-40 (Thermo Fisher Scientific: 85124), 0.5% Sodium deoxycholate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS; SAFC Biosciences) and 1% (v/v) Penicillin/Streptomycin (P/S; Gibco, Life Technologies). Primary fibroblasts were maintained in F-DMEM culture medium comprising DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... and at 30 DIV by neurosphere medium (Advanced DMEM/F12, 1x B27 -RA, 1x L-glutamate, 1x NEAA, 1x P-S [all from Thermo Fisher Scientific]) supplemented with 20 ng/ml bFGF2 and 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Systems Biology 2023Quote: Human induced Pluripotent Stem Cells were cultured in mTeSR Plus Medium (100-0276 Stem Cell) + Penicillin/Streptomycin (P/S) 1% (15140122 Thermo Fisher Scientific) on Matrigel coating (354277 Corning) ...