Labshake search
Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed using an antibody against PCNA and the HistoMouse kit-plus kit (Invitrogen/Thermo Fisher Scientific), using AEC single solution chromogen ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed using an antibody against PCNA and the HistoMouse kit-plus kit (Invitrogen/Thermo Fisher Scientific), using AEC single solution chromogen ...
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bioRxiv - Cell Biology 2019Quote: 1 μg of total RNA isolated from Control or IFT88 KD cells was converted to cDNA using One-cycle labeling kit and amplified using IVT labeling kit following manufacturer’s instructions (Affymetrix). The normalized cRNA was fragmented ...
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bioRxiv - Immunology 2021Quote: ... Endotoxin was removed using the Proteus NoEndo HC Spin column kit following the manufacturer’s recommendations (VivaProducts) and removal was confirmed using the Pierce LAL Chromogenic Endoxotin Quantification Kit (ThermoFisher). IL-2 proteins were concentrated and stored at -80 °C until use.
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bioRxiv - Plant Biology 2019Quote: ... and individual clones isolated and multiplied using a CloneJet PCR cloning kit and GeneJet plasmid kit (both from Thermofisher.com), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Peroxidase activity was recorded using a commercially available kit (Amplex® Red Hydrogen Peroxide / Peroxidase Assay Kit, ThermoFisher Scientific). Samples were diluted 1:5 to bring values within the range of the kit standards ...
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bioRxiv - Systems Biology 2020Quote: ... For each step proteome was quantified using a commercially available assay kit (Micro BCA™ Protein Assay Kit, Thermofisher) and samples were diluted to be in the linear range of BSA protein standard range (0.5 to 20 μg/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Overall cell death was evaluated using a commercially available kit (Live/Dead Viability/Cytotoxicity Kit; Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions as described previously (Pellegrini et al. ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... plasmids were linearized with XhoI digestion and transcribed with an SP6 in vitro transcription kit (mMESSAGE mMACHINE Kit, Ambion). In vitro transcribed viral RNAs ...
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bioRxiv - Neuroscience 2020Quote: Cytokines released by primary microglia in the culture medium were quantified by ELISA using the following commercial kits according to manufacturer’s instruction: Mouse TNF alpha Uncoated ELISA kit (88-7324-22, Invitrogen), Rat IL-6 DuoSet ELISA (DY506 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... labelled with Annexin-V-FITC and propidium iodide according to the manufacturer’s instructions (FITC Annexin V kit and Dead Cell Apoptosis Kit, Invitrogen), and analysed by cytofluorimetry with the LSR Fortessa X20 instrument (Becton Dickinson).
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bioRxiv - Zoology 2019Quote: ... DNA was extracted twice using a specific forensic kit and procedure (ChargeSwitch® Forensic DNA Purification Kit, Thermo Fisher). In short ...
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bioRxiv - Zoology 2021Quote: ... RNA was extracted using the RNeasy micro extraction kit and reverse transcribed it to generate cDNA using a standard kit following the manufacturer’s instructions (Superscript III, Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 100 mg of ground tissue per sample was extracted using the Kit Pure link RNA Mini Kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The elastase levels within the supernatant were measured using an Elastase Assay kit (EnzChek Elastase Assay kit, Life Technologies) according to manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: Plasmids for microinjection were extracted using the PureLink HQ Mini Plasmid Purification Kit or HiPure Plasmid Midiprep kit (Invitrogen). Genomic DNAs were extracted from mix-staged animals with PureLink Genomic DNA Mini Kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... DNA concentration was assessed with Qubit broad range kit (Qubit™ dsDNA BR Assay Kit, Q32850, Thermo Fisher Scientific) or nanodrop ...
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bioRxiv - Genetics 2020Quote: ... and mRNA was synthesized and purified using an In Vitro RNA Transcription Kit (mMESSAGE mMACHINE T7 Ultra kit, Ambion). SgRNA oligos were amplified and transcribed in vitro using the GeneArt Precision gRNA Synthesis Kit (Thermo ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Pierce™ LDH Cytotoxicity Assay Kit and Amplex™ Red Cholesterol Assay Kit were purchased from Invitrogen (Waltham, MA). 96-well black microplates were purchased from Greiner bio-one (Kremsmünster ...
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Modeling PPRV pathogenesis in mice to assess the contribution of innate cells and anti-viral T cellsbioRxiv - Immunology 2020Quote: ... The mRNA was converted into cDNA using a first strand synthesis kit (Verso cDNA synthesis kit, Thermo Fisher Scientific). The expression of hypoxanthine phosphoribosyltransferase 1 (HPRT 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... following the instructions provided in the kit Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher). For DNA damage analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were measured using the Bio-Rad Protein Assay Kit or BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts (25 μg or 10 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of the DNA was measured using a QUBIT kit 1x dsDNA HS Assay kit (ThermoFisher, Waltham, MA) and fragment size was assessed on a Femto Pulse (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane and cytosolic fractions were isolated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific kit #89842).
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bioRxiv - Molecular Biology 2023Quote: ... cells were permeabilized with 1X permeabilization buffer (PB) (kit Foxp3/Transcription Factor Staining Buffer kit, Thermo Fisher, 00-5523) for 30 min RT ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined with the MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific, United Kingdom).
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bioRxiv - Microbiology 2024Quote: ... Sequencing cleanup was done using the QIAquick PCR Purification Kit followed by quantitation using the Qubit dsDNA HS Assay Kit on the Qubit 2.0 Fluorometer from Invitrogen by Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified DNA/ cDNA was then purified with MicroCLEAN Kit (Clent Life Science) and further processed with the BigDye Terminator v1.1 Cycle Sequencing Kit (ThermoFisher). Sequencing was performed with the ABI PRISM 3730 DNA Analyzer (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... kit and cDNA was synthesized using the SuperScript™ III Reverse Transcriptase kit (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher) and quantified using Quibit dsDNA HS Assay kit (ThermoFisher).
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bioRxiv - Cell Biology 2019Quote: ... we performed a Click reaction according to the manufacturer’s instructions to reveal the EdU or EU (Click-iT EdU Alexa Fluor 488 imaging kit; Click-iT RNA Alexa Fluor 488 imaging kit, both from Invitrogen) to label nascent DNA or RNA respectively.
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthesized by in vitro transcription with 1μl of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). the pCS2+_ancBE4max-SpymacCas9 has been linearized using KpnI restriction enzyme ...
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bioRxiv - Neuroscience 2020Quote: A lysis reaction followed by reverse transcription was performed using the kit Taqman Gene Expression Cells-to-CT Kit (ThermoFisher) with ‘Lysis Solution’ followed by the ‘Stop Solution’ at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: The PCR products were purified to remove unincorporated primers and dNTP’s using the PCR purification kit (Purelink PCR purification kit, Invitrogen, USA). The purified products were processed for sequencing on a next generation sequencer platform @ 40X coverage (MiSeq ...
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Nulliparity affects the expression of a limited number of genes and pathways in Day 8 equine embryosbioRxiv - Developmental Biology 2022Quote: ... RNA was extracted later from each hemi-embryo using PicoPure RNA isolation kit (PicoPure RNA isolation kit, Applied Biosystems, France), which included a DNAse treatment ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Macherey Nagel Nucleo Spin Tissue DNA kit and quantified using a Qubit fluorometer (Qubit dsDNA BR Assay Kit, Q32853, Invitrogen). Thirty-seven blood samples at T0 (pre-stress ...
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bioRxiv - Genetics 2019Quote: ... using the Ion PGM™ sequencing 200 Kit v2 and the Ion 314™ Chip Kit v2 (Thermo Fisher Scientific). Data from the PGM runs were processed using the Ion Torrent Suite 5.4 software to generate sequencing reads ...
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bioRxiv - Molecular Biology 2019Quote: ... we used either the nickel-based protocol or the Pierce Cobalt kit (Pull-Down PolyHis Protein:Protein Interaction Kit cat. #21277 from Thermo Fisher). Similar results were obtained using either the nickel or cobalt protocol ...
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bioRxiv - Immunology 2020Quote: ... The plasmids were purified using standard plasmid kits and prepared for Sanger sequencing using BigDye Terminator v3.1 Cycle Sequencing Kit (ThermoFisher # 4337455) with pSB_EF1a_seq.FOR and pSB_bGH_seq.REV primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA was bar-coded using the Ion Xpress Barcode Adapters kit and quantified by quantitative PCR using the Ion Library TaqMan Quantitation Kit (ThermoFisher) after purification of libraries by Agencourt AMPure XP beads (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was assessed using the Click-iT TUNEL Alexa Fluor 546 Imaging Assay kit and EdU incorporation by Click-iT EdU Alexa Fluor 546 Imaging Assay kit (both from ThermoFisher), according to the manufacturers’ instructions.
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bioRxiv - Pathology 2021Quote: ... Apoptosis was assessed using the Click-iT TUNEL Alexa Fluor 546 Imaging Assay kit and EdU incorporation by Click-iT EdU Alexa Fluor 546 Imaging Assay kit (both from ThermoFisher), according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Immunology 2021Quote: Supernatants from MSC and T-cell cocultures were analyzed using IL-10 Ready SET-Go! ELISA kits kits (Affymetrix, eBioscience) for secretion of IL-10 as previously described [31] ...
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bioRxiv - Neuroscience 2021Quote: ... SP was labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers nCoV_IP2-12669Fw ...