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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used: anti-ZO-1 (Zymed, #40-2200, 1:100 or Invitrogen, #33-9100, 1:100); anti-N-cadherin (BD Biosciences ...
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bioRxiv - Bioengineering 2019Quote: ... mouse anti-zona occludens-1 (ZO-1) (Invitrogen 1:100, Cat #33-9100), anti-βIII-tubulin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-Vimentin (Invitrogen, V9, WB 1:1000-1:2500, IF 1:100), mouse anti-Actin (NeoMarkers ...
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bioRxiv - Neuroscience 2023Quote: ... or mouse anti- zonula occludens 1 (ZO-1) (1:100, Invitrogen, 35-2500). Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The following chemicals were used: EU (100 μM, Invitrogen), EdU (10 μM ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were then flushed with 25 ug/ml neutravidin (Invitrogen, #A-2666) in assay buffer containing 200 ug/ml kappa-casein and instantly rinsed with assay buffer followed by warm up to RT ...
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bioRxiv - Systems Biology 2021Quote: ... both cell types were stained with Hoechst (10 ug/ml, Life Technologies) to pick cells in G0/G1 phase ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfected cells were selected with zeocin (300 ug/ml; Gibco/Life Technologies) and sorted for double positive cells (anti-HA-AF488 and anti-EGFR-AF647 ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfected cells were selected with zeocin (300 ug/ml; Gibco/Life Technologies) and sorted for double positive cells (anti-HA-AF488 and anti-EGFR-AF647 ...
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bioRxiv - Biophysics 2022Quote: ... and HCS CellMask™ Deep Red Stain (2.5 ug/ml, ThermoFisher, #H32721). Staining with the Golgi dye from the Golgi Staining Kit was performed following the instructions of the provider ...
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bioRxiv - Genetics 2023Quote: ... and selected infected cells with 2 ug/ml puromycin (Thermo Fisher Scientific). Cells were maintained in 0.5 ug/ml puromycin for the duration of the experiment to maintain gRNA expression ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK-BAC16 derivatives were also maintained in 10 ug/mL blasticidin (Gibco). For lytic infection ...
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bioRxiv - Biophysics 2024Quote: ... 20 ug/ml BSA) using Zeba 5K desalting spin columns (ThermoFisher Scientific). The variants were subjected to oxidation by mixing σ70 variant at 2.5 μM final concentration with hydrogen peroxide at 5 mM final concentration in buffer R ...
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bioRxiv - Microbiology 2021Quote: ... 1 ug of RNA was reverse transcribed with Superscript IV VILO master mix (Thermo Fisher 11756050) and diluted 1:20 prior to use in qPCR reactions ...
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bioRxiv - Bioengineering 2022Quote: ... 1 ug DNase-free RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) with random primers (Promega ...
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared with 1-2 ug of RNA using random hexamers and Revertaid RT (ThermoFisher). LT mRNA levels were quantified by Taqman qPCR with normalization to TATA-Box Binding Protein and compared to a standard curve (Maru et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was reverse transcribed using the SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific). Gene expression was analyzed by qRT-PCR using the PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: A total of 1 ug of RNA was used for oligo(dT) reverse transcription (SuperScriptII, Invitrogen). RT-qPCR were performed using SsoAdvanced Universal SYBR Green Supermix (Bio Rad ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 1 ug of RNA using Oligo dT(12-18) primers (Invitrogen, 18418012) and SuperScript II reverse transcriptase (Invitrogen ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... The cells were transfected with plasmids (total 1 ug) using 1.5 uL of Lipofectamine 2000 (Invitrogen) after 24 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The UGS were transferred into fresh 1× DEPC-PBS and then placed into RNALater (ThermoFisher AM7020) for storage at −80 °C until processing ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-ZO-1 (Invitrogen cat. # 33-9100; 1:100), mouse anti-ARL13b (UC Davis / NIH ...
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bioRxiv - Bioengineering 2019Quote: ZO-1 (1:100, 61-7300, Thermo Fisher Scientific, USA)
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bioRxiv - Pathology 2019Quote: ... and anti-ZO-1 (1:100, 402200, Thermo Fisher Scientific,). Retina samples were incubated for 24 hours at 4° C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... anti-TFAP2C (Rabbit, 1:100, Thermo Fisher Scientific, 14572-1). PBS was used to wash off the primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and rabbit anti-ZO-1 (Invitrogen, 61-7300, 1:100). The following secondary antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 rabbit anti-TRX-1 (MA532569, Thermo Fisher Scientific), 1:100 rabbit anti-AGT (MA529010 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:100 N2 supplement (15410294, Gibco, 1:67 20% Glucose) was prepared and supplemented with 10 μM SB431542 (SB ...
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bioRxiv - Cell Biology 2024Quote: ... anti-CD90 (Thy-1) 1:100 (Invitrogen, #14-0909-82), and anti-DLK-1 1:100 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.05 % D-Glucose monohydrate (Thermo Scientific Chemicals; CAS 14431-43-7) in 500 mL Erlenmeyer flasks without baffles ...
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bioRxiv - Neuroscience 2020Quote: ... P8 to P10 mouse cerebella were dissected in ice cold Gey’s balanced salt solution complemented with 4.5 mg/ml D-Glucose and penicillin-streptomycin (100 IU/mL, Thermo Fisher Scientific). They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301], 100 μL of Collagenase Type I [480U/mL, Gibco, 17100-017] in 9 mL of PBS [Gibco ...
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bioRxiv - Genetics 2021Quote: ... Wash buffer was discarded and replaced by 250 μL of elution buffer (100 mM NaHCO3, 1% SDS, 100 units/mL RNase OUT (Invitrogen) in 0,1% DEPC water according to [36] ...
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bioRxiv - Cell Biology 2021Quote: ... 338-AC, 100 ng/mL) in DE medium (RPMI 1640, 1% Penicillin/Streptomycin, 1% L-Glutamine (Gibco, ref. 25-030-081). DE differentiation was induced by threating MES differentiated cultures for 2 days with ACTIVIN A (R&D ...
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bioRxiv - Physiology 2020Quote: ... primary adipocytes were attached to poly-L-lysine (25-100 μg ml-1) coated coverslips and incubated with the Ca2+ fluorophore Fluo-4 AM (1 μM; Molecular Probes) in modified Hank’s solution for 1 hour at 21-23°C in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 1 x 106 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 10% heat inactivated FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 100 mg of mice faeces were dissolved in 1 mL of 1x PBS with 1% protease inhibitors (Thermo Scientific) and vortexed for 15 minutes at 4°C ...
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bioRxiv - Physiology 2022Quote: ... and 10 u/ml of penicillin and 10 ug/ml of streptomycin (Cat. 15149-122, Gibco) of in a 5% CO2 environment ...
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bioRxiv - Genetics 2022Quote: ... containing 10% fetal bovine serum (FBS) and Penicillin (10,000 units/ml)/ Streptomycin (10,000μg/ml, 1:100 dilution of stock, Gibco #15140-122). After reaching confluency ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were placed in a 100 mL beaker with 40 mL of 1% sodium dodecyl sulfate (SDS) (Thermo Scientific) solution and agitated in an orbital shaker at 100 RPM at ambient temperature for 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Gradient elution to 100%B (acetonitrile) was performed using an UltimateTM 3000 quaternary UPLC pump (Thermo Scientific) at a flow rate of 0.7 mL/min at 50°C ...
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bioRxiv - Cell Biology 2019Quote: ... for 1h at room temperature. Sections were stained with primary antibodies against NEO1 (R&D cat. no. AF1079; dilution: 1:100) and Endomucin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1% n-Dodecyl β-D-maltoside (DDM) and 0.05% Triton X-100) supplemented with 1 X HALT protease and phosphatase inhibitor cocktail (Thermo Fisher). The lysate was centrifuged at 14,000 rpm for 20 min at 4°C and supernatant was transferred to new tubes and normalized for total protein amounts determined by BCA assay ...
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bioRxiv - Developmental Biology 2023Quote: ... The treated surface was coated with poly-D-lysine solution (PLL, Thermofisher Scientific, 100 µg/ml) for 30 minutes and then rinse with deionized water and Hepes buffer (0.1M pH 8.3) ...
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bioRxiv - Neuroscience 2023Quote: ... and concentration and purity were assessed with a Nanodrop ND-100 (Thermo Fisher Scientific). For library preparation and transcriptome analysis ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FBS (Atlanta Biologics) and 100 U mL-1 penicillin-streptomycin (Thermo Fisher). The Expi293F cell line was maintained in an 8% CO2 incubator at 37 °C shaking in baffled flasks ...
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bioRxiv - Bioengineering 2022Quote: ... 100 U/mL of streptomycin) (HyClone),1% (v/v) MEM non-essential amino acid (Gibco). Human monocytic U937 cells (ATCC ...
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bioRxiv - Developmental Biology 2022Quote: ... Digestion was then quenched with 1 mL 100% FBS (Thermo Fisher, Cat#35-015-CV) for 15 min on ice following by washing in 1 mL media containing DMEM-F12 ...