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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Recombinant Mouse Cd83 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were grown in HL5 reinforced with 10% dialysed FCS (Gibco).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) foetal calf serum (FCS; Gibco) and 100 U/ml penicillin and 100 μg/ml streptomycin (P/S ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Fc block (anti-CD16/32, ThermoFisher) followed by staining with a NP311-325 IAb MHC Class II tetramer or NP366-374 H-2Db MHC Class I tetramer (generated by the NIH Tetramer Core Facility) ...
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bioRxiv - Immunology 2024Quote: ... and were used fresh or after freezing in FCS (Invitrogen) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) and antibiotics (Gibco). All cell lines were authenticated by short tandem repeat profiling and tested for mycoplasma contamination ...
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bioRxiv - Cell Biology 2024Quote: ... DPBS supplemented with FCS (10% vol/vol, Gibco, #10270-106) was added to the cell suspension ...
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bioRxiv - Neuroscience 2024Quote: ... macrophages were differentiated in RPMI 1640 with 10% FCS (Gibco) containing M-CSF (100 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (v/v) Foetal Calf Serum (FCS, Gibco) and Penicillin (100U/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) (Gibco A5670401 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Gibco) and 0.05 mM 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % (v/v) heat-inactivated FCS (10500064, Gibco), 100 U/ml streptomycin and 100 µg/ml penicillin (15140122 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4% FCS and 1% L-Glutamine (L-Gln; Invitrogen). These adherent cells were cultured to confluence for five days at 37 °C in an atmosphere containing 5% CO2.
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS; Invitrogen), 100 µg/ml streptomycin and 100 units/ml penicillin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... containing 2mM EDTA (Thermo) and 2% FCS (Gibco or Omega) with a 23 gauge needle into the peritoneal cavity and then retrieving the solution back out with the same syringe and a 21 gauge needle ...
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bioRxiv - Molecular Biology 2021Quote: ... We immunoprecipitated GFP-tagged Znf684 with 1 μg anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and/or AlexaFluor-594-tagged goat anti-guinea pig IgG (1:500; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000; Molecular Probes) for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged U2OS cells were seeded in GlutaMAX- supplemented DMEM (Gibco, 10566-016) with 10% FBS and 1% Penicillin/Streptomycin at 6x103 cells per well ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and human recombinant epidermal growth factor (EGF; Gibco 10450-013). All cells were cultured at 37 °C and 5% CO2.