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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Recombinant Human TIMP Metallopeptidase Inhibitor 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... frozen left-ventricular tissue was placed in Isolation solution (Supplemental Table 2) containing protease and phosphatase inhibitors (1:100 ratio, Fisher Scientific), 10 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Developmental Biology 2020Quote: HEK293 cells expressing wild-type or mutant ATF6 were lysed in SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (Thermo Scientific). Protein concentrations of the total cell lysates were determined by BCA protein assay (Pierce) ...
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bioRxiv - Bioengineering 2022Quote: ... reactions were quenched with an equal volume of stop buffer containing 2 mM PMSF and 2x Pierce protease inhibitor minitablet (Thermo Fisher) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Developmental Biology 2022Quote: ... Individual blastocysts derived from embryo culture was washed in cold PBS 3 times to remove the media and transferred in minimal volume to 2 µL of RNA buffer containing 0.1 IU RNase inhibitor (Thermo Fisher Scientific). RNA was extracted by three repeats of freezing in liquid nitrogen and thawing with vortex ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Genetics 2022Quote: ... 2% Triton X-100) with with an appropriate volume of 100x Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific; catalogue #78430) was added to each tube ...
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bioRxiv - Plant Biology 2019Quote: ... RNA (3 µg in GR24 assay/2 μg in Pseudomonas infection) was DNAseI treated and reverse transcribed with Maxima RT and Ribolock Rnase inhibitor (Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: The cell culture supernatant was mixed with an equal volume of 2×RNA lysis buffer (distilled water containing 0.4 U/uL SUPERase ITM RNase Inhibitor (Thermo Fisher Scientific), 2% Triton X-100 ...
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Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolismbioRxiv - Physiology 2021Quote: ... the pellets were resuspended in lysis buffer (Tris-HCl, 137mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA) with protease and phosphatase inhibitors (Thermo Scientific) added along with the PMSF (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed with ice-cold solubilization buffer (2% CHAPS, 150 mM NaCl, 25 mM Tris-HCl, pH 7.2) containing 1X HALT™ protease inhibitor cocktail (ThermoFisher 78430), using 400 μL of lysis buffer for each well of a 6-well plate ...
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bioRxiv - Genetics 2020Quote: ... and a cell suspension containing an equal amount of each iPSC line was prepared in E8 medium containing 10 μM Rock inhibitor and seeded at 2 x 105 cells per cm2 on 0.01% Geltrex-(Thermo Fisher, A1413202) coated plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock) was also added to all lysis buffers and subsequent steps to avoid RNA degradation ...
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bioRxiv - Microbiology 2019Quote: ... with 1x protease inhibitors (Protease Inhibitor Cocktail Kit, Thermo Scientific #78410) was added to the plate ...
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bioRxiv - Microbiology 2021Quote: ... EDTA free protease inhibitor (Pierce™ Protease inhibitor, Thermo Scientific, USA) and 10% glycerol] and lysed with lysozyme followed by sonication ...
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bioRxiv - Biochemistry 2020Quote: ... protease inhibitor cocktail (PierceTM Protease Inhibitor Mini Tablets, Thermo Scientific, USA), and phosphatase inhibitor cocktail 2 (catalog#P5726) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with protease inhibitors cocktail and phosphatase inhibitor (Thermo Fisher Scientific), and kept on ice for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with protease inhibitors cocktail and phosphor inhibitor (Thermo Fisher Scientific), and kept on ice for 30 mins ...
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bioRxiv - Cell Biology 2021Quote: ... pH 8.0) containing protease inhibitors (Protease Inhibitor Tablets; ThermoFisher Scientific, www.thermofisher.com). Fibroblast lysates were centrifuged to remove insoluble material and protein concentration of the supernatants determined with the bicinchronic acid (BCA ...
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bioRxiv - Microbiology 2020Quote: ... containing 1x protease inhibitor and phosphatase inhibitor cocktail (Thermo Fisher Scientific). After centrifugation at 15,000 rpm at 4 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with Halt protease inhibitor and Halt phosphatase inhibitor (Thermo Scientific). Protein concentrations were measured using Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... with 1x protease inhibitors (Protease Inhibitor Cocktail Kit, Thermo Scientific #78410) was added to the plate ...
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bioRxiv - Microbiology 2019Quote: ... with 1x protease inhibitors (Protease Inhibitor Cocktail Kit, Thermo Scientific #78410) was added to the plate ...
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bioRxiv - Microbiology 2019Quote: ... and 1x protease inhibitors (Protease Inhibitor Cocktail Kit, Thermo Scientific #78410). Cells were lysed by passage through a chilled 26G needle five times and cleared by centrifugation for 10 min at 21,000 × g at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% glycerol) containing fresh protease inhibitor and phosphatase inhibitor cocktail (ThermoFisher), followed by centrifugation to remove cellular debris ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with Halt Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (ThermoFisher). Samples were sonicated using a Bioruptor Plus sonicator (30 secs ON ...
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bioRxiv - Immunology 2023Quote: ... EDTA-free protease inhibitor cocktail and phosphatase inhibitor cocktail (Thermo Fisher). The lysates were centrifuged at 6,000 g at 4°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... containing protease inhibitor and phosphatase inhibitor cocktail (Cat# PPC1010, Thermo Fisher) and PMSF (Cat# 8553 ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 7.5) containing 1x protease inhibitor (Halt Protease Inhibitor, Thermofisher Scientific) at a concentration of 10 mg/mL ...
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bioRxiv - Pathology 2024Quote: ... containing a protease inhibitor/ phosphatase inhibitor cocktail (#78440, Thermo Fisher Scientific). Lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Cancer Biology 2022Quote: Organoid cultures established from human ACCs were dissociated from Matrigel by incubation with a solution of 2 mg/mL Dispase-II (Thermo Fisher, 17105041) and 200 U/mL Collagenase-III (Worthington ...
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bioRxiv - Cell Biology 2022Quote: Male mouse neuroblastoma Neuro-2a (N2a; ATCC, CCL-131) and female human epithelial HeLa (ATCC, CCL-2) cells were maintained in MEM (Thermo Fisher Scientific), supplemented with 10% FCS ...
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bioRxiv - Microbiology 2021Quote: To facilitate the analyses of invasion assays, HeLa cells (human cervical carcinoma, ATCC® CCL-2™) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... All parasites obtained from other sources or generated in this study were maintained under standard conditions (37) at 2% hematocrit with O+ human erythrocytes in RPMI1640 (Thermo Fisher Scientific) containing hypoxanthine ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... isolated naïve CD8+ T cells were plated at 2.5 × 105 cells/well in a 24-well plate in 2 ml cRPMI on day 0 and activated with DynaBeads Human T-Activator anti-CD3/CD28 coated microbeads (Thermo Fisher Scientific) and recombinant human IL-2 (rIL-2 ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...